FastQCFastQC Report
Thu 26 May 2016
SRR1544934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544934_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences476643
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT25270.5301661830762227Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT21630.45379875504308254Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT15820.33190459106710896Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.1928067757210323No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT7300.15315445731920954Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC6830.14329382787537004No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC6720.14098602098425866No Hit
GCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC5650.1185373539525389No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG5210.10930612638809341No Hit
AGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC4920.10322190822061794No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT4810.10091410132950657Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC253.4141245E-546.05
CCCCTTA206.307718E-446.018
GCGACGG453.092282E-1046.01
GTCGATG206.307718E-446.019
GGTCGTT206.307718E-446.025
GTCGAGG600.046.01
GGTCGAG206.307718E-446.07
TTTACGG206.307718E-446.01
CGAAAGG206.307718E-446.01
CATGCGG750.046.01
TTTCGCA253.4141245E-546.013
ATTACGG301.8590945E-646.01
TCTTAGG301.8590945E-646.01
CCGGATA301.8590945E-646.019
CCGTGTC301.8590945E-646.015
ATAGCGG453.092282E-1046.01
TACGGGT301.8590945E-646.03
TACGGGA800.046.03
TATGCGT206.307718E-446.019
TCGATGG453.092282E-1046.01
Produced by FastQC (version 0.11.5)