##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544934_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	476643
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.922438806402276	34.0	33.0	34.0	31.0	34.0
2	33.19354527392619	34.0	33.0	34.0	31.0	34.0
3	33.23167024376735	34.0	33.0	34.0	31.0	34.0
4	36.5324467158859	37.0	37.0	37.0	35.0	37.0
5	36.52474073887585	37.0	37.0	37.0	35.0	37.0
6	36.64667686297711	37.0	37.0	37.0	35.0	37.0
7	36.46372232467486	37.0	37.0	37.0	35.0	37.0
8	36.51234781587058	37.0	37.0	37.0	35.0	37.0
9	38.301863239363634	39.0	39.0	39.0	37.0	39.0
10	38.088980641696196	39.0	39.0	39.0	35.0	39.0
11	37.23035689184568	39.0	35.0	39.0	35.0	39.0
12	37.105760495800844	39.0	35.0	39.0	35.0	39.0
13	37.09369066576033	39.0	35.0	39.0	35.0	39.0
14	38.30906149885764	40.0	35.0	41.0	35.0	41.0
15	38.3870653717772	40.0	35.0	41.0	35.0	41.0
16	38.38726887838487	40.0	35.0	41.0	35.0	41.0
17	38.4752802411868	40.0	36.0	41.0	35.0	41.0
18	38.45826121436799	40.0	36.0	41.0	35.0	41.0
19	38.34825854990003	40.0	35.0	41.0	35.0	41.0
20	38.24491076130354	40.0	35.0	41.0	35.0	41.0
21	38.18589384507902	40.0	35.0	41.0	35.0	41.0
22	38.16443124099168	40.0	35.0	41.0	35.0	41.0
23	38.142846952541	40.0	35.0	41.0	35.0	41.0
24	38.10153091517131	40.0	35.0	41.0	35.0	41.0
25	38.059791080536165	40.0	35.0	41.0	35.0	41.0
26	38.01035156290977	40.0	35.0	41.0	35.0	41.0
27	37.982886562899274	40.0	35.0	41.0	35.0	41.0
28	38.029986803540595	40.0	35.0	41.0	35.0	41.0
29	38.04674567758259	40.0	35.0	41.0	35.0	41.0
30	37.873049640926226	40.0	35.0	41.0	35.0	41.0
31	37.844309053106834	40.0	35.0	41.0	35.0	41.0
32	37.716540471589845	40.0	35.0	41.0	35.0	41.0
33	37.58986075532422	39.0	35.0	41.0	35.0	41.0
34	37.48005320543887	39.0	35.0	41.0	35.0	41.0
35	37.45277282997967	39.0	35.0	41.0	35.0	41.0
36	37.40034365342615	39.0	35.0	41.0	34.0	41.0
37	37.354361650123884	39.0	35.0	41.0	34.0	41.0
38	37.31131685559213	39.0	35.0	41.0	35.0	41.0
39	37.227960968691455	39.0	35.0	41.0	34.0	41.0
40	37.16014921020554	39.0	35.0	41.0	34.0	41.0
41	37.129566572885786	39.0	35.0	41.0	34.0	41.0
42	36.99856286570872	38.0	35.0	41.0	34.0	41.0
43	36.90413160373697	38.0	35.0	41.0	34.0	41.0
44	36.840864126820286	38.0	35.0	41.0	34.0	41.0
45	36.80700650172142	38.0	35.0	41.0	34.0	41.0
46	36.86246939533361	38.0	35.0	41.0	34.0	41.0
47	36.79534788090877	38.0	35.0	41.0	34.0	41.0
48	36.82265762845568	38.0	35.0	41.0	34.0	41.0
49	36.729827145263855	37.0	35.0	41.0	34.0	41.0
50	36.61051982301219	37.0	35.0	41.0	34.0	41.0
51	36.51403251490109	37.0	35.0	40.0	33.0	41.0
52	36.245936686366946	36.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	6.0
15	6.0
16	7.0
17	29.0
18	34.0
19	67.0
20	129.0
21	223.0
22	380.0
23	667.0
24	1174.0
25	1818.0
26	2552.0
27	2885.0
28	2908.0
29	3179.0
30	3728.0
31	4565.0
32	5775.0
33	8123.0
34	23893.0
35	120181.0
36	16112.0
37	24110.0
38	44216.0
39	208473.0
40	1398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.97088806507176	22.039136208860718	24.52485403121414	21.46512169485338
2	26.7852459807445	23.436198580488963	27.53318521409105	22.24537022467549
3	26.582368774953164	25.132436645455826	25.942896465488847	22.342298114102167
4	26.43236132703092	26.8725230413538	23.641803194424337	23.053312437190936
5	23.170381186758224	36.19018846390275	21.082235551555357	19.557194797783666
6	86.00042379726546	2.759927241142742	8.098094380909822	3.1415545806819782
7	85.52879198897287	2.3967623567323972	6.2501704630090025	5.824275191285721
8	75.10274985681107	4.486166795694052	12.771822936663288	7.639260410831588
9	51.588505443277256	17.157285431654298	15.97149229087598	15.282716834192467
10	45.44764110665634	17.802002756780233	24.689127921735974	12.06122821482745
11	42.08474686505414	15.763160268796565	24.384287611482808	17.76780525466649
12	19.338372744381015	35.56225518889399	28.95353545525687	16.145836611468123
13	13.374580136496286	40.11681698881553	28.650583350641885	17.8580195240463
14	11.616241086095883	21.26979731161477	50.51369683389875	16.6002647683906
15	12.62538209939095	16.436200678495226	51.446890020413605	19.491527201700222
16	13.266532813866982	19.324525903034345	28.918288950010805	38.490652333087866
17	17.713047291159214	21.448757245989135	37.014075524029515	23.82411993882214
18	23.25870725049985	26.726921406587316	28.597084190893398	21.417287152019433
19	27.488287880027613	20.976286235190695	26.22151169743393	25.313914187347763
20	18.489519409704958	31.489815228588274	27.35716248848719	22.663502873219578
21	18.88939940374662	23.67369288964697	25.865480034323383	31.571427672283033
22	16.287871635584704	31.5496084071307	22.93708289012951	29.22543706715508
23	16.237729285859647	22.059486869627793	38.98892882094146	22.7138550235711
24	16.962170849042156	21.25741907465336	37.61746212574191	24.16294795056258
25	14.194061383467291	30.724672343871617	30.67201238662899	24.409253886032104
26	18.37958388143747	29.424957462922986	27.468776421766393	24.726682233873152
27	18.73897235457145	26.633350327184075	38.00811089221913	16.619566426025347
28	16.390254341299464	22.79882427728929	32.90009503968379	27.910826341727457
29	21.00901513291919	27.028614707443516	27.77005012136966	24.192320038267635
30	27.106031138608984	23.22555875151843	25.15845192313744	24.509958186735144
31	30.898177461957903	22.366844787398534	24.291136133332493	22.44384161731107
32	29.321106152822974	23.798734063019914	25.64791678467952	21.232242999477595
33	20.13414652056151	23.287030335072583	30.269824585696213	26.308998558669693
34	20.36010179526396	20.474023535434277	33.31843748885434	25.847437180447418
35	32.97709186959632	18.31181827908938	27.261703203445762	21.449386647868533
36	23.433051571091994	25.986954597046424	31.497577851767467	19.082415980094115
37	21.95878256892475	25.732676237771244	30.39004034466047	21.918500848643536
38	20.59549809815732	27.313733758809004	28.616176047901682	23.474592095131996
39	28.643450129342085	22.844560813858592	26.43236132703092	22.0796277297684
40	16.28241681929662	21.5368735091043	32.0751589764247	30.105550695174376
41	23.964266757300535	22.535734291702596	25.58560599861951	27.914392952377355
42	23.69886896482273	20.435210419538315	28.854509559565543	27.011411056073413
43	23.906571585022753	24.820882715155786	28.495330887058028	22.77721481276343
44	17.730670543782242	30.415845821715624	28.10678012684546	23.746703507656676
45	23.583898221520087	25.90806956149571	28.767861900835634	21.740170316148564
46	24.83493935712892	21.242523230174363	29.308518115235096	24.614019297461624
47	24.045879200995294	18.04767929037036	32.69952564078356	25.206915867850782
48	21.32581407888084	17.317782910899773	30.50878749923947	30.847615510979914
49	22.175506616062755	22.947992522705675	31.56513365348909	23.31136720774248
50	20.601582316324794	24.651363808972334	28.318468958948312	26.42858491575456
51	19.893085600753604	20.100998021580093	32.700574643915886	27.305341733750417
52	22.980092018554767	17.68829081723638	35.46322929320267	23.868387871006185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95.0
1	128.0
2	161.0
3	888.0
4	1615.0
5	1163.0
6	711.0
7	840.5
8	970.0
9	991.0
10	1012.0
11	955.5
12	899.0
13	870.0
14	816.5
15	792.0
16	705.0
17	618.0
18	695.5
19	773.0
20	763.5
21	754.0
22	752.0
23	750.0
24	837.5
25	925.0
26	969.0
27	1013.0
28	1347.5
29	1682.0
30	2222.5
31	2763.0
32	3253.0
33	3743.0
34	4722.0
35	5701.0
36	6278.0
37	6855.0
38	7395.0
39	9445.0
40	10955.0
41	16837.5
42	22720.0
43	34050.0
44	45380.0
45	51614.5
46	57849.0
47	61299.0
48	64749.0
49	64612.5
50	64476.0
51	56296.5
52	48117.0
53	40663.0
54	33209.0
55	27613.0
56	22017.0
57	18937.5
58	15858.0
59	14850.5
60	13843.0
61	12992.0
62	12141.0
63	10416.0
64	7396.0
65	6101.0
66	4671.0
67	3241.0
68	2840.5
69	2440.0
70	2151.0
71	1862.0
72	1365.0
73	868.0
74	804.0
75	740.0
76	613.0
77	486.0
78	330.5
79	175.0
80	136.5
81	98.0
82	54.5
83	11.0
84	6.0
85	1.0
86	3.5
87	6.0
88	3.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	476643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.78295185080345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.67201828847165	18.66022311168434
2	12.179741668849358	6.524188693440338
3	4.589514221393104	3.687622152304476
4	2.2759135828942063	2.438227356289804
5	1.30119481099946	1.7424918985756914
6	0.8005253118448843	1.2864258529494572
7	0.6081551315207805	1.1401732723738072
8	0.4497869151981016	0.9637297034297736
9	0.37846446316220717	0.9122755944702213
>10	7.124891538683979	47.12718485118633
>50	0.5163644628840943	9.198367372606457
>100	0.09637758563694022	4.144522307079577
>500	0.004701345640826352	0.8583268133047481
>1k	0.002350672820413176	1.3162410203049828
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	2527	0.5301661830762227	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT	2163	0.45379875504308254	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	1582	0.33190459106710896	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	919	0.1928067757210323	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	730	0.15315445731920954	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	683	0.14329382787537004	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	672	0.14098602098425866	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	565	0.1185373539525389	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG	521	0.10930612638809341	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	492	0.10322190822061794	No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	481	0.10091410132950657	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46219078010166936	0.0
2	0.0	0.0	0.0	1.6003591786725075	0.0
3	0.0	0.0	0.0	2.5222231313582704	0.0
4	0.0	0.0	0.0	3.1031610660389433	0.0
5	0.0	0.0	0.0	6.0783437499344375	0.0
6	0.0	0.0	0.0	6.704598619931479	0.0
7	0.0	0.0	0.0	9.981264804056705	0.0
8	0.0	0.0	0.0	13.269050421384557	0.0
9	0.0	0.0	0.0	16.648099311224545	0.0
10	0.0	0.0	0.0	17.932498746441258	0.0
11	0.0	0.0	0.0	19.241444854954338	0.0
12	0.0	0.0	0.0	20.37898385164578	0.0
13	0.0	0.0	0.0	20.73690372039451	0.0
14	0.0	0.0	0.0	21.048038049441615	0.0
15	0.0	0.0	0.0	22.094103972994464	0.0
16	0.0	0.0	0.0	23.175626202419842	0.0
17	0.0	0.0	0.0	25.000052450156616	0.0
18	0.0	0.0	0.0	25.6219015069979	0.0
19	0.0	0.0	0.0	26.507889552558204	0.0
20	0.0	0.0	0.0	27.467937219260538	0.0
21	0.0	0.0	0.0	28.18230835237274	0.0
22	0.0	0.0	0.0	29.105640909443757	0.0
23	0.0	0.0	0.0	29.678396619692307	0.0
24	0.0	0.0	0.0	30.143314807938015	0.0
25	0.0	0.0	0.0	30.679145607928785	0.0
26	0.0	0.0	0.0	31.130007154201362	0.0
27	0.0	0.0	0.0	31.588211722400203	0.0
28	0.0	0.0	0.0	31.9878819158154	0.0
29	0.0	0.0	0.0	32.65966352175528	0.0
30	0.0	0.0	0.0	33.29158300866686	0.0
31	0.0	0.0	0.0	33.7890202940146	0.0
32	0.0	0.0	0.0	34.231489815228585	0.0
33	0.0	0.0	0.0	34.67249073205733	0.0
34	0.0	0.0	0.0	35.3549721699469	0.0
35	2.0980062646467062E-4	0.0	0.0	35.819260956313215	0.0
36	2.0980062646467062E-4	0.0	0.0	36.33264308927227	0.0
37	2.0980062646467062E-4	0.0	0.0	36.76525198104242	0.0
38	2.0980062646467062E-4	0.0	0.0	37.16072616192832	0.0
39	2.0980062646467062E-4	0.0	0.0	37.66697507358757	0.0
40	2.0980062646467062E-4	0.0	0.0	38.162733953923585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	25	3.4141245E-5	46.0	5
CCCCTTA	20	6.307718E-4	46.0	18
GCGACGG	45	3.092282E-10	46.0	1
GTCGATG	20	6.307718E-4	46.0	19
GGTCGTT	20	6.307718E-4	46.0	25
GTCGAGG	60	0.0	46.0	1
GGTCGAG	20	6.307718E-4	46.0	7
TTTACGG	20	6.307718E-4	46.0	1
CGAAAGG	20	6.307718E-4	46.0	1
CATGCGG	75	0.0	46.0	1
TTTCGCA	25	3.4141245E-5	46.0	13
ATTACGG	30	1.8590945E-6	46.0	1
TCTTAGG	30	1.8590945E-6	46.0	1
CCGGATA	30	1.8590945E-6	46.0	19
CCGTGTC	30	1.8590945E-6	46.0	15
ATAGCGG	45	3.092282E-10	46.0	1
TACGGGT	30	1.8590945E-6	46.0	3
TACGGGA	80	0.0	46.0	3
TATGCGT	20	6.307718E-4	46.0	19
TCGATGG	45	3.092282E-10	46.0	1
>>END_MODULE