FastQCFastQC Report
Thu 26 May 2016
SRR1544933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544933_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences285399
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT18450.6464633723313676Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT15270.5350404171002701Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT12170.42642055508253357Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTTG8770.30728909351469347Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.2011219380586477No Hit
ACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC4720.16538249958829568No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT4700.16468172628495545Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC4000.1401546606680472No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA3750.13139499437629423No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC3690.12929267446627354No Hit
AGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC3560.12473764799456201No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT3120.10932063532107682Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGT351.01596015E-746.0000043
AATGCGG351.01596015E-746.0000041
CTTATGG351.01596015E-746.0000041
GCGAGAC301.8564515E-646.020
TTAGGGT206.303442E-446.03
ATAGGGT600.046.03
ACGTTTG253.4106575E-546.022
ACCATTC206.303442E-446.019
GGGTAGT206.303442E-446.06
CCTACCT206.303442E-446.011
GTGATGA206.303442E-446.08
AATGGGC501.6370905E-1146.03
AGGGTCA253.4106575E-546.05
TACGGGT206.303442E-446.03
TACGGGC301.8564515E-646.03
CGCGACC206.303442E-446.09
TCGATGG253.4106575E-546.01
GGGACTA650.046.06
CTAACGG253.4106575E-546.01
GTTAAGG501.6370905E-1146.01
Produced by FastQC (version 0.11.5)