##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544933_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	285399
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.925157411203266	34.0	33.0	34.0	31.0	34.0
2	33.25124124471354	34.0	33.0	34.0	31.0	34.0
3	33.36548130862407	34.0	34.0	34.0	31.0	34.0
4	36.60267204860563	37.0	37.0	37.0	35.0	37.0
5	36.60468677185274	37.0	37.0	37.0	35.0	37.0
6	36.39313732704039	37.0	37.0	37.0	35.0	37.0
7	36.210533323522505	37.0	37.0	37.0	35.0	37.0
8	36.37265021951724	37.0	37.0	37.0	35.0	37.0
9	38.258140357884926	39.0	39.0	39.0	37.0	39.0
10	38.135049527153214	39.0	39.0	39.0	35.0	39.0
11	37.37361378280933	39.0	35.0	39.0	35.0	39.0
12	37.326290561634764	39.0	35.0	39.0	35.0	39.0
13	37.31649725472058	39.0	35.0	39.0	35.0	39.0
14	38.498813941184096	41.0	35.0	41.0	35.0	41.0
15	38.52863534910774	41.0	35.0	41.0	35.0	41.0
16	38.55926615019674	41.0	35.0	41.0	35.0	41.0
17	38.67067158609525	41.0	36.0	41.0	35.0	41.0
18	38.65840454942028	40.0	36.0	41.0	35.0	41.0
19	38.58337275183165	40.0	35.0	41.0	35.0	41.0
20	38.490201437286046	40.0	35.0	41.0	35.0	41.0
21	38.436827038637134	40.0	35.0	41.0	35.0	41.0
22	38.41824603449907	40.0	35.0	41.0	35.0	41.0
23	38.37777988009769	40.0	35.0	41.0	35.0	41.0
24	38.356458852343565	40.0	35.0	41.0	35.0	41.0
25	38.293648541165176	40.0	35.0	41.0	35.0	41.0
26	38.23593285190207	40.0	35.0	41.0	35.0	41.0
27	38.30583498891026	40.0	35.0	41.0	35.0	41.0
28	38.34555481974359	40.0	35.0	41.0	35.0	41.0
29	38.367965549984405	40.0	36.0	41.0	35.0	41.0
30	38.21685429871864	40.0	35.0	41.0	35.0	41.0
31	38.185477174061575	40.0	35.0	41.0	35.0	41.0
32	38.10349720917032	40.0	35.0	41.0	35.0	41.0
33	37.97786957908052	40.0	35.0	41.0	35.0	41.0
34	37.88246980543029	40.0	35.0	41.0	35.0	41.0
35	37.844070932273766	40.0	35.0	41.0	35.0	41.0
36	37.80129923370439	40.0	35.0	41.0	35.0	41.0
37	37.74914067673678	40.0	35.0	41.0	35.0	41.0
38	37.75826824901279	40.0	35.0	41.0	35.0	41.0
39	37.717574343287815	40.0	35.0	41.0	35.0	41.0
40	37.67983419703643	40.0	35.0	41.0	35.0	41.0
41	37.662612693106844	40.0	35.0	41.0	35.0	41.0
42	37.56889127151812	40.0	35.0	41.0	35.0	41.0
43	37.48633667251812	39.0	35.0	41.0	35.0	41.0
44	37.346605979698595	39.0	35.0	41.0	35.0	41.0
45	37.34046370169482	39.0	35.0	41.0	35.0	41.0
46	37.301549760160334	39.0	35.0	41.0	35.0	41.0
47	37.21841351931857	39.0	35.0	41.0	35.0	41.0
48	37.192257155771394	39.0	35.0	41.0	35.0	41.0
49	37.10767732192475	39.0	35.0	41.0	35.0	41.0
50	37.00001751933258	38.0	35.0	41.0	35.0	41.0
51	36.90318466427703	38.0	35.0	41.0	34.0	41.0
52	36.368470106762814	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	3.0
16	7.0
17	10.0
18	19.0
19	36.0
20	79.0
21	122.0
22	163.0
23	293.0
24	533.0
25	970.0
26	1279.0
27	1524.0
28	1374.0
29	1377.0
30	1636.0
31	1911.0
32	2667.0
33	3631.0
34	12240.0
35	68803.0
36	8745.0
37	14050.0
38	26406.0
39	136907.0
40	611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.706488109628978	21.660202032943353	23.898121577160396	22.735188280267273
2	26.775847147327074	23.263220964334142	27.133241532030596	22.827690356308185
3	26.43211784203869	24.935266066103946	26.117821015490595	22.51479507636677
4	26.475916173497456	26.395677630264995	23.82208767374798	23.306318522489565
5	22.948223364482708	35.67216423323137	21.345554819743587	20.034057582542335
6	84.31949656445889	2.869666677178266	8.945020830486442	3.8658159278764113
7	83.88256440982624	2.3304216202579546	7.174517079597336	6.612496890318466
8	72.68665972901096	4.566589231216647	14.1657819403712	8.58096909940119
9	49.85301279962439	16.886884677241333	16.78281984169531	16.477282681438968
10	43.59265449423439	17.55226892876289	26.545643117179807	12.309433459822914
11	40.411844470373055	15.709585527629738	24.992729476977846	18.88584052501936
12	18.775819116394942	33.83578779182828	30.368011100249127	17.02038199152765
13	13.318196629981186	38.21947519087313	29.548807108644386	18.9135210705013
14	11.662970087491546	21.124110455888072	49.36106994067954	17.85184951594084
15	12.579931954912245	16.585201770153365	50.706905069744465	20.127961205189926
16	13.0999057459907	20.062789287979285	29.7527321399164	37.084572826113614
17	17.13811190648881	21.80245901352142	37.03727062813815	24.02215845185162
18	22.47450061142471	27.32350148388747	28.905146829526384	21.29685107516144
19	26.39147299044496	21.408274030392537	26.304226714179098	25.89602626498341
20	17.986397990182166	32.18231318259699	27.214180848566393	22.617107978654445
21	18.44575489052169	23.958037694595987	25.759025084180394	31.83718233070193
22	16.21239037277636	31.84594199699368	23.023206107940112	28.918461522289846
23	15.971324356427319	22.41703720055081	38.241549549928344	23.37008889309353
24	17.67105000367906	21.693138378200345	36.32563533859614	24.310176279524455
25	14.298228094702504	30.042501900847583	31.144117533698434	24.515152470751474
26	18.764957130193167	27.935626964355166	27.383067214671392	25.91634869078028
27	19.943307439759774	25.838562854109508	37.259766151948675	16.95836355418204
28	17.064530709638085	22.386203175203836	32.560380379749056	27.988885735409024
29	22.019698737556894	26.185445639262927	27.28846281872046	24.506392804459722
30	26.94648544669042	22.48711453088483	25.20331185463159	25.36308816779316
31	31.322814726050197	21.687532191773624	23.739046037302163	23.25060704487402
32	29.482584031478737	22.742896786604017	26.11922256209727	21.65529661981997
33	20.203995108602342	22.303511925409687	30.936688635909725	26.555804330078242
34	21.101685710181183	19.57224797564112	33.881338056545395	25.4447282576323
35	32.70579084019215	17.715549108441163	27.47066387758892	22.107996173777764
36	23.809824140939526	25.512703268056303	31.30389384686001	19.37357874414416
37	22.28248872630948	24.826295817434538	31.630804592868227	21.26041086338775
38	20.835391854911894	27.103809053290306	28.282159362856913	23.778639728940888
39	29.198420456974272	22.50533463677168	26.51586025178785	21.780384654466204
40	16.588705636670063	21.216962918580652	32.15007761064334	30.044253834105938
41	23.90933395001384	22.434906919785984	25.708569406339898	27.947189723860276
42	23.503235820728175	19.99376311760027	29.09505639473159	27.407944666939972
43	24.641992438656057	24.514451697448134	28.191409220074355	22.652146643821457
44	17.664743043948995	28.850136125214178	29.30563877238533	24.1794820584515
45	23.330845588106474	25.36098584788314	28.897087936537968	22.411080627472415
46	25.09329044600717	20.01548709000382	29.7867196451284	25.104502818860613
47	23.721877091370327	17.24953486171991	33.18617093963188	25.842417107277882
48	21.028104513330458	16.648972140757326	30.204380533919178	32.11854281199303
49	21.855017011271936	21.697343018020383	32.28357492492966	24.164065045778017
50	20.84870654767536	23.398820598530477	29.235211055399635	26.517261798394525
51	19.59642465460636	19.22326287057768	32.99661176107835	28.183700713737608
52	22.62306455173284	17.179107144734214	36.07440810934867	24.123420194184284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53.0
1	87.5
2	122.0
3	578.0
4	1034.0
5	748.5
6	463.0
7	514.5
8	566.0
9	579.5
10	593.0
11	599.5
12	606.0
13	561.5
14	493.0
15	469.0
16	429.0
17	389.0
18	420.0
19	451.0
20	460.5
21	470.0
22	447.0
23	424.0
24	463.5
25	503.0
26	565.0
27	627.0
28	752.0
29	877.0
30	1189.5
31	1502.0
32	1732.0
33	1962.0
34	2419.5
35	2877.0
36	3351.5
37	3826.0
38	4136.0
39	5282.0
40	6118.0
41	9267.0
42	12416.0
43	18866.5
44	25317.0
45	29249.0
46	33181.0
47	36580.0
48	39979.0
49	40246.0
50	40513.0
51	35410.5
52	30308.0
53	25574.0
54	20840.0
55	17201.5
56	13563.0
57	11541.0
58	9519.0
59	8910.5
60	8302.0
61	7813.0
62	7324.0
63	6425.0
64	4586.5
65	3647.0
66	2825.5
67	2004.0
68	1754.0
69	1504.0
70	1327.5
71	1151.0
72	809.5
73	468.0
74	476.5
75	485.0
76	396.0
77	307.0
78	202.0
79	97.0
80	73.0
81	49.0
82	26.0
83	3.0
84	1.5
85	0.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	285399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.11573971877967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47439394270003	22.95453032421277
2	10.756289685570271	6.908923997631386
3	3.7050775709703463	3.569739207215162
4	1.8198084182504528	2.337779739943027
5	1.2175696611315978	1.9551575163192583
6	0.9055401601605969	1.7449255253171876
7	0.7047939077876454	1.5844484388522733
8	0.6873377119291278	1.7659487244173946
9	0.6862466996879705	1.983538835104538
>10	7.783281328416505	45.756992841600706
>50	0.20401928909642367	4.18782126076125
>100	0.05018656309323791	3.133858212537535
>500	0.0021820244823146915	0.5084110315733412
>1k	0.0032730367234720375	1.6079243445141715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	1845	0.6464633723313676	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT	1527	0.5350404171002701	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	1217	0.42642055508253357	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTTG	877	0.30728909351469347	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	574	0.2011219380586477	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	472	0.16538249958829568	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	470	0.16468172628495545	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	400	0.1401546606680472	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	375	0.13139499437629423	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	369	0.12929267446627354	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	356	0.12473764799456201	No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	312	0.10932063532107682	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5434496967403529	0.0
2	0.0	0.0	0.0	1.9078553183437923	0.0
3	0.0	0.0	0.0	2.942897487377321	0.0
4	0.0	0.0	0.0	3.6464738839309176	0.0
5	0.0	0.0	0.0	7.043472471872712	0.0
6	0.0	0.0	0.0	7.729879922494472	0.0
7	0.0	0.0	0.0	11.40263280530065	0.0
8	0.0	0.0	0.0	15.103066233588764	0.0
9	0.0	0.0	0.0	18.98394878748699	0.0
10	0.0	0.0	0.0	20.417730966121116	0.0
11	0.0	0.0	0.0	21.790195480712967	0.0
12	0.0	0.0	0.0	23.095736144835826	0.0
13	0.0	0.0	0.0	23.507790847199885	0.0
14	0.0	0.0	0.0	23.831548113343075	0.0
15	0.0	0.0	0.0	24.97661169100102	0.0
16	0.0	0.0	0.0	26.10450632272713	0.0
17	0.0	0.0	0.0	27.847679914785967	0.0
18	0.0	0.0	0.0	28.50885952648748	0.0
19	0.0	0.0	0.0	29.356094450225825	0.0
20	0.0	0.0	0.0	30.32351199548702	0.0
21	0.0	0.0	0.0	31.083500642959507	0.0
22	0.0	0.0	0.0	31.98399433775171	0.0
23	0.0	0.0	0.0	32.60067484469111	0.0
24	0.0	0.0	0.0	33.09507041019765	0.0
25	0.0	0.0	0.0	33.6245046408712	0.0
26	0.0	0.0	0.0	34.06318872876219	0.0
27	0.0	0.0	0.0	34.5309549087418	0.0
28	0.0	0.0	0.0	34.93495071811744	0.0
29	0.0	0.0	0.0	35.58281563705549	0.0
30	0.0	0.0	0.0	36.20545271707329	0.0
31	0.0	0.0	0.0	36.686533589816364	0.0
32	0.0	0.0	0.0	37.15429976979597	0.0
33	0.0	0.0	0.0	37.576866071710135	0.0
34	0.0	0.0	0.0	38.259068882511855	0.0
35	0.0	0.0	0.0	38.71527230298634	0.0
36	0.0	0.0	0.0	39.206164001976184	0.0
37	0.0	0.0	0.0	39.622072957508614	0.0
38	0.0	0.0	0.0	40.037981913041044	0.0
39	3.50386651670118E-4	0.0	0.0	40.53483018510927	0.0
40	3.50386651670118E-4	0.0	0.0	40.9917343788871	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGGT	35	1.01596015E-7	46.000004	3
AATGCGG	35	1.01596015E-7	46.000004	1
CTTATGG	35	1.01596015E-7	46.000004	1
GCGAGAC	30	1.8564515E-6	46.0	20
TTAGGGT	20	6.303442E-4	46.0	3
ATAGGGT	60	0.0	46.0	3
ACGTTTG	25	3.4106575E-5	46.0	22
ACCATTC	20	6.303442E-4	46.0	19
GGGTAGT	20	6.303442E-4	46.0	6
CCTACCT	20	6.303442E-4	46.0	11
GTGATGA	20	6.303442E-4	46.0	8
AATGGGC	50	1.6370905E-11	46.0	3
AGGGTCA	25	3.4106575E-5	46.0	5
TACGGGT	20	6.303442E-4	46.0	3
TACGGGC	30	1.8564515E-6	46.0	3
CGCGACC	20	6.303442E-4	46.0	9
TCGATGG	25	3.4106575E-5	46.0	1
GGGACTA	65	0.0	46.0	6
CTAACGG	25	3.4106575E-5	46.0	1
GTTAAGG	50	1.6370905E-11	46.0	1
>>END_MODULE