FastQCFastQC Report
Thu 26 May 2016
SRR1544932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544932_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1217463
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT35560.29208279840947937Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT26950.2213619633615149Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT26390.2167622342527042Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24950.2049343594014767No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17880.1468627794027416No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA17100.1404560138583267No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT15000.12320702970028657No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA14420.11844302455187551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGG2150.046.0000041
CGGGTAC253.4172765E-546.05
ATTACGG501.6370905E-1146.01
TGGCGAA301.8614955E-646.027
ATATGCG301.8614955E-646.018
GTTACGA206.3116034E-446.042
TACGATT253.4172765E-546.044
ATCGGTT206.3116034E-446.033
GCGTATA206.3116034E-446.041
CCGCATG253.4172765E-546.024
AATACGG405.6115823E-946.01
ATAGCGG1400.044.357141
ATTGCGG1800.043.4444471
TCGACGG900.043.4444471
CTCGAGG1450.042.8275871
GCGGGAT4800.042.6458324
AATGCGG1300.042.461541
CCGTAGG650.042.461541
TACGGGA2450.042.24493
TTACGGG2950.042.1016962
Produced by FastQC (version 0.11.5)