##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544932_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1217463
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94491167288041	34.0	33.0	34.0	31.0	34.0
2	33.26563928431501	34.0	33.0	34.0	31.0	34.0
3	33.33040182740667	34.0	34.0	34.0	31.0	34.0
4	36.599413698814665	37.0	37.0	37.0	35.0	37.0
5	36.5944648831217	37.0	37.0	37.0	35.0	37.0
6	36.669851979074515	37.0	37.0	37.0	35.0	37.0
7	36.49544832163277	37.0	37.0	37.0	35.0	37.0
8	36.51856360316494	37.0	37.0	37.0	35.0	37.0
9	38.33329226432343	39.0	39.0	39.0	37.0	39.0
10	38.175863250053595	39.0	39.0	39.0	35.0	39.0
11	37.593013504311834	39.0	35.0	39.0	35.0	39.0
12	37.52213496426585	39.0	35.0	39.0	35.0	39.0
13	37.503833792074175	39.0	35.0	39.0	35.0	39.0
14	38.82792413403939	41.0	36.0	41.0	35.0	41.0
15	38.871570635000815	41.0	36.0	41.0	35.0	41.0
16	38.8624106030327	41.0	36.0	41.0	35.0	41.0
17	38.89158684904593	41.0	36.0	41.0	35.0	41.0
18	38.87574160364627	41.0	36.0	41.0	35.0	41.0
19	38.826982010952285	41.0	36.0	41.0	35.0	41.0
20	38.75160230742125	41.0	35.0	41.0	35.0	41.0
21	38.70649785660837	41.0	35.0	41.0	35.0	41.0
22	38.68307866440294	41.0	35.0	41.0	35.0	41.0
23	38.65796250070844	41.0	35.0	41.0	35.0	41.0
24	38.601214164208685	41.0	35.0	41.0	35.0	41.0
25	38.558383293783876	40.0	35.0	41.0	35.0	41.0
26	38.48925018665865	40.0	35.0	41.0	35.0	41.0
27	38.45303635510894	40.0	35.0	41.0	35.0	41.0
28	38.486069802531986	40.0	36.0	41.0	35.0	41.0
29	38.48986540042695	40.0	36.0	41.0	35.0	41.0
30	38.42354223495909	40.0	35.0	41.0	35.0	41.0
31	38.36816149648901	40.0	35.0	41.0	35.0	41.0
32	38.29726652883907	40.0	35.0	41.0	35.0	41.0
33	38.20429039732624	40.0	35.0	41.0	35.0	41.0
34	38.103278703336365	40.0	35.0	41.0	35.0	41.0
35	38.030585734433	40.0	35.0	41.0	35.0	41.0
36	37.98521762057656	40.0	35.0	41.0	35.0	41.0
37	37.94569855511009	40.0	35.0	41.0	35.0	41.0
38	37.853590622466555	40.0	35.0	41.0	35.0	41.0
39	37.75554328961127	40.0	35.0	41.0	35.0	41.0
40	37.67452234688036	40.0	35.0	41.0	35.0	41.0
41	37.62114906161419	40.0	35.0	41.0	35.0	41.0
42	37.54612665846929	40.0	35.0	41.0	35.0	41.0
43	37.46864422163138	39.0	35.0	41.0	34.0	41.0
44	37.39715786023887	39.0	35.0	41.0	34.0	41.0
45	37.33691537237682	39.0	35.0	41.0	34.0	41.0
46	37.32211245844843	39.0	35.0	41.0	34.0	41.0
47	37.2565548193251	39.0	35.0	41.0	34.0	41.0
48	37.228733029258386	39.0	35.0	41.0	34.0	41.0
49	37.18202113739802	39.0	35.0	41.0	34.0	41.0
50	37.107728941249135	39.0	35.0	41.0	34.0	41.0
51	37.030390245945874	38.0	35.0	41.0	34.0	41.0
52	36.81819817111485	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	9.0
14	17.0
15	20.0
16	36.0
17	79.0
18	110.0
19	205.0
20	327.0
21	525.0
22	795.0
23	1294.0
24	2102.0
25	3431.0
26	4802.0
27	5792.0
28	6293.0
29	6802.0
30	7986.0
31	10070.0
32	12761.0
33	18291.0
34	59348.0
35	217295.0
36	47825.0
37	73844.0
38	127350.0
39	603901.0
40	6146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.831612952508614	21.95155006764066	23.25072712682028	19.966109853030442
2	28.45261005878618	24.121636550761707	25.499419694890115	21.926333695562
3	29.073573488475624	24.50160703035739	23.96269948244834	22.462119998718645
4	26.721797705556554	26.714487421794335	21.72427416685353	24.839440705795575
5	24.511710006792814	36.05588013763047	20.406780329258467	19.02562952631825
6	87.03541709275765	3.4688528522016684	6.872816668761186	2.622913386279501
7	87.03960613176746	3.4013353999259115	5.317697539884169	4.241360928422465
8	75.85692542607045	6.360850391346595	10.646976540560164	7.135247642022796
9	49.05068983615929	20.002004167683126	15.883768130941146	15.063537865216436
10	39.748476955767856	21.816186611009943	23.110106836922352	15.325229596299847
11	38.65686267262332	18.148806164951214	24.43983923946765	18.754491922957822
12	24.271292022837653	27.75903661959337	27.361488603760442	20.608182753808535
13	17.30360594120725	30.8273023492295	29.97561322192132	21.893478487641925
14	17.34738550576075	23.42683104127189	39.048661027070224	20.17712242589713
15	20.5505218639088	18.925421142162023	40.16130264328361	20.362754350645567
16	20.665432953609265	21.456257808245507	27.536935414053648	30.341373824091573
17	22.031141808827044	23.27922901969095	30.65752306230251	24.032106109179498
18	24.99903487826735	26.933549520601446	26.81067104298036	21.256744558150842
19	28.496143209280284	23.351674753154718	24.855539757676414	23.296642279888587
20	23.6026885416641	28.884327490855988	26.019599774284725	21.493384193195194
21	21.730352380318745	24.23942247115518	26.093934682203894	27.936290466322177
22	21.67277362843881	27.30234922950431	24.96568684222847	26.059190299828416
23	22.525530550004394	21.20376553537972	33.91158499272668	22.359118921889205
24	22.015207032985806	21.81922571774255	30.578259873195325	25.587307376076318
25	19.53036765799043	27.216186446733904	28.45474564730098	24.79870024797468
26	19.203540477205465	26.705205825556916	28.769909229274322	25.321344467963296
27	20.366614837576172	26.265521005566494	32.63499588899211	20.732868267865225
28	20.217041503520026	25.67806988795553	28.859193256797127	25.24569535172732
29	22.30014382367267	25.984608977849838	28.698695566107553	23.016551632369936
30	26.963447759808716	23.848116944827073	24.90482256955653	24.283612725807686
31	27.882654339392655	25.945100590325946	24.696766965402645	21.47547810487875
32	27.36009225742384	24.895623111338907	26.00645769111669	21.73782694012056
33	20.58920887123469	24.69865613985805	29.121377815999338	25.590757172907924
34	23.281364608205752	24.170919362641822	29.59925681519685	22.948459213955577
35	28.30607583146264	25.17177113390715	24.443946140457655	22.078206894172553
36	24.804942737479497	27.06809159703416	26.42289745150366	21.704068213982683
37	24.681407155700008	27.36518481465145	25.41613174281272	22.537276286835823
38	24.375443031944297	25.866658781416767	25.24807735430153	24.509820832337407
39	27.594432027913783	21.410917621315804	28.07888206869531	22.915768282075103
40	19.87970065620064	20.96737231439477	31.325962267436463	27.826964761968124
41	21.717620987249713	24.57011013887075	25.97598448577082	27.736284388108718
42	22.309096867830892	22.065968329222326	28.571874463536055	27.053060339410724
43	22.77752999475138	25.607677604986762	28.034691814042812	23.580100586219046
44	21.374284064484918	26.46569135981956	27.224975214852527	24.935049360843
45	22.54483298465744	26.08802074477828	26.494275390710026	24.872870879854254
46	23.869062139876117	22.766277086038755	28.421808301361107	24.942852472724017
47	23.187891541673135	20.108044351245173	31.84523882861327	24.858825278468423
48	22.03393450150025	20.378606988467002	29.685091045888047	27.9023674641447
49	21.40640003022679	23.822982710768212	30.335213472606558	24.43540378639844
50	20.802849860734987	24.29461922046091	28.796850499768784	26.10568041903532
51	21.665545482696395	21.047867573798957	30.157138245679743	27.129448697824905
52	23.355617378105126	19.701379015214428	32.40747357414558	24.53553003253487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	193.0
1	492.5
2	792.0
3	2999.0
4	5206.0
5	3429.0
6	1652.0
7	1703.5
8	1755.0
9	1708.5
10	1662.0
11	1607.5
12	1553.0
13	1595.5
14	1487.0
15	1336.0
16	1382.0
17	1428.0
18	1404.5
19	1381.0
20	1457.5
21	1534.0
22	1840.0
23	2146.0
24	2754.0
25	3362.0
26	4603.5
27	5845.0
28	7932.0
29	10019.0
30	11469.0
31	12919.0
32	13794.5
33	14670.0
34	16735.0
35	18800.0
36	20442.0
37	22084.0
38	24293.0
39	32021.0
40	37540.0
41	46317.0
42	55094.0
43	67641.0
44	80188.0
45	90757.0
46	101326.0
47	108736.5
48	116147.0
49	119250.5
50	122354.0
51	113818.0
52	105282.0
53	94928.0
54	84574.0
55	76772.5
56	68971.0
57	64929.0
58	60887.0
59	59708.5
60	58530.0
61	57728.0
62	56926.0
63	51501.5
64	37868.5
65	29660.0
66	24623.5
67	19587.0
68	16069.0
69	12551.0
70	10968.0
71	9385.0
72	7930.0
73	6475.0
74	5061.5
75	3648.0
76	3191.5
77	2735.0
78	2186.0
79	1637.0
80	1127.0
81	617.0
82	485.0
83	353.0
84	344.5
85	336.0
86	206.0
87	76.0
88	49.5
89	12.5
90	2.0
91	2.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1217463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.62146008743595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.46873601794316	15.941056992419611
2	11.579478261861583	5.2388941066806805
3	4.306681981897384	2.9227030368831364
4	2.059995079490744	1.8640038588405725
5	1.1755500387017055	1.3296329140637206
6	0.7387022509842468	1.002631409228354
7	0.5442616326071285	0.8618394959401419
8	0.4401581645551659	0.7965616281314999
9	0.36670602690353826	0.7465883176278544
>10	6.738740322515724	40.58489631914121
>50	1.4196870135062651	20.101978576080967
>100	0.14965150996106533	5.603348278633984
>500	0.007282311920245801	1.186514846147436
>1k	0.00436938715214748	1.8193502201808098
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	3556	0.29208279840947937	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT	2695	0.2213619633615149	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	2639	0.2167622342527042	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2495	0.2049343594014767	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1788	0.1468627794027416	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1710	0.1404560138583267	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1500	0.12320702970028657	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1442	0.11844302455187551	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.213801980019105E-5	0.0	0.0	0.2247296221733227	0.0
2	8.213801980019105E-5	0.0	0.0	0.8933331033468779	0.0
3	8.213801980019105E-5	0.0	0.0	1.4250125055135145	0.0
4	8.213801980019105E-5	0.0	0.0	1.753646722734079	0.0
5	8.213801980019105E-5	0.0	0.0	3.6653269955637255	0.0
6	8.213801980019105E-5	0.0	0.0	4.035194498723986	0.0
7	8.213801980019105E-5	0.0	0.0	5.594009838492012	0.0
8	8.213801980019105E-5	0.0	0.0	7.352256290334902	0.0
9	8.213801980019105E-5	0.0	0.0	8.937437934458789	0.0
10	8.213801980019105E-5	0.0	0.0	9.774095804143535	0.0
11	8.213801980019105E-5	0.0	0.0	10.574284393036995	0.0
12	8.213801980019105E-5	0.0	0.0	11.247569741339161	0.0
13	8.213801980019105E-5	0.0	0.0	11.546962823510858	0.0
14	8.213801980019105E-5	0.0	0.0	11.766682026476369	0.0
15	8.213801980019105E-5	0.0	0.0	12.528922850222143	0.0
16	1.642760396003821E-4	0.0	0.0	13.290424431789713	0.0
17	1.642760396003821E-4	0.0	0.0	14.28618364582743	0.0
18	2.4641405940057314E-4	0.0	0.0	14.80233896225183	0.0
19	2.4641405940057314E-4	0.0	0.0	15.389461527783595	0.0
20	2.4641405940057314E-4	0.0	0.0	16.09223442519403	0.0
21	2.4641405940057314E-4	0.0	0.0	16.712458612705273	0.0
22	2.4641405940057314E-4	0.0	0.0	17.355352893681367	0.0
23	2.4641405940057314E-4	0.0	0.0	17.85072729109632	0.0
24	2.4641405940057314E-4	0.0	0.0	18.287208728314535	0.0
25	2.4641405940057314E-4	0.0	0.0	18.718022642166538	0.0
26	2.4641405940057314E-4	0.0	0.0	19.1310126057219	0.0
27	2.4641405940057314E-4	0.0	0.0	19.53973139224765	0.0
28	2.4641405940057314E-4	0.0	0.0	19.95304990788221	0.0
29	2.4641405940057314E-4	0.0	0.0	20.452941896386175	0.0
30	2.4641405940057314E-4	0.0	0.0	20.9365705569697	0.0
31	2.4641405940057314E-4	0.0	0.0	21.388986770029152	0.0
32	2.4641405940057314E-4	0.0	0.0	21.849370371009222	0.0
33	2.4641405940057314E-4	0.0	0.0	22.2710669646634	0.0
34	2.4641405940057314E-4	0.0	0.0	22.804060575146842	0.0
35	2.4641405940057314E-4	0.0	0.0	23.236763663454248	0.0
36	2.4641405940057314E-4	0.0	0.0	23.687044287998898	0.0
37	2.4641405940057314E-4	0.0	0.0	24.15301327432538	0.0
38	2.4641405940057314E-4	0.0	0.0	24.636231244809903	0.0
39	2.4641405940057314E-4	0.0	0.0	25.146719037868092	0.0
40	2.4641405940057314E-4	0.0	0.0	25.655646208550074	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGG	215	0.0	46.000004	1
CGGGTAC	25	3.4172765E-5	46.0	5
ATTACGG	50	1.6370905E-11	46.0	1
TGGCGAA	30	1.8614955E-6	46.0	27
ATATGCG	30	1.8614955E-6	46.0	18
GTTACGA	20	6.3116034E-4	46.0	42
TACGATT	25	3.4172765E-5	46.0	44
ATCGGTT	20	6.3116034E-4	46.0	33
GCGTATA	20	6.3116034E-4	46.0	41
CCGCATG	25	3.4172765E-5	46.0	24
AATACGG	40	5.6115823E-9	46.0	1
ATAGCGG	140	0.0	44.35714	1
ATTGCGG	180	0.0	43.444447	1
TCGACGG	90	0.0	43.444447	1
CTCGAGG	145	0.0	42.827587	1
GCGGGAT	480	0.0	42.645832	4
AATGCGG	130	0.0	42.46154	1
CCGTAGG	65	0.0	42.46154	1
TACGGGA	245	0.0	42.2449	3
TTACGGG	295	0.0	42.101696	2
>>END_MODULE