FastQCFastQC Report
Thu 26 May 2016
SRR1544931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544931_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences745810
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT29100.39017980450785056Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA22520.3019535806706802No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT20950.28090264276424287Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT20690.27741650018101127Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.2154704281251257No Hit
TGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTTG13470.18060900229280918Illumina Single End Adapter 1 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9860.1322052533487081No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA7740.1037797830546654No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG206.310023E-446.01
TACCAGG600.046.01
CGCATGT206.310023E-446.039
ATTACGG301.8605188E-646.01
TCGATCA453.092282E-1046.016
AATGCGG800.046.01
CCGTAGG501.6370905E-1146.01
ACTACGG453.092282E-1046.01
AACTCCG206.310023E-446.013
ATTGCGG1300.046.01
CATGCGG1400.044.357141
TTGCGGG4250.043.8352972
ATAGCGG800.043.1251
GCATAGG1600.043.1251
CTTGCGG2200.042.8636361
CGTTTTT10000.042.551
TACACGG601.8189894E-1242.1666681
CCTCGAT554.7293724E-1141.8181814
CCATAGG554.7293724E-1141.818181
GCCTACG554.7293724E-1141.8181825
Produced by FastQC (version 0.11.5)