##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544931_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	745810
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91475308724742	34.0	33.0	34.0	31.0	34.0
2	33.28434051568094	34.0	34.0	34.0	31.0	34.0
3	33.41611000120674	34.0	34.0	34.0	31.0	34.0
4	36.62871508829326	37.0	37.0	37.0	35.0	37.0
5	36.63243185261662	37.0	37.0	37.0	35.0	37.0
6	36.41712366420402	37.0	37.0	37.0	35.0	37.0
7	36.22516458615465	37.0	37.0	37.0	35.0	37.0
8	36.36154650648288	37.0	37.0	37.0	35.0	37.0
9	38.26042691838404	39.0	39.0	39.0	37.0	39.0
10	38.178847159464205	39.0	39.0	39.0	35.0	39.0
11	37.64756037060377	39.0	37.0	39.0	35.0	39.0
12	37.63123315589761	39.0	37.0	39.0	35.0	39.0
13	37.61824057065473	39.0	37.0	39.0	35.0	39.0
14	38.86117375739129	41.0	37.0	41.0	35.0	41.0
15	38.876603960794306	41.0	37.0	41.0	35.0	41.0
16	38.88875316769687	41.0	37.0	41.0	35.0	41.0
17	38.947619366862874	41.0	37.0	41.0	35.0	41.0
18	38.94748394363176	41.0	37.0	41.0	35.0	41.0
19	38.93246403239431	41.0	37.0	41.0	35.0	41.0
20	38.87731191590351	41.0	36.0	41.0	35.0	41.0
21	38.82913878869954	41.0	35.0	41.0	35.0	41.0
22	38.81310387364074	41.0	35.0	41.0	35.0	41.0
23	38.77266193802711	41.0	35.0	41.0	35.0	41.0
24	38.72631903567933	41.0	35.0	41.0	35.0	41.0
25	38.67435137635591	41.0	35.0	41.0	35.0	41.0
26	38.58202357168716	40.0	35.0	41.0	35.0	41.0
27	38.65527681312935	41.0	36.0	41.0	35.0	41.0
28	38.67962215577694	41.0	36.0	41.0	35.0	41.0
29	38.688969040372214	41.0	36.0	41.0	35.0	41.0
30	38.63791180059265	41.0	35.0	41.0	35.0	41.0
31	38.575133076788994	41.0	35.0	41.0	35.0	41.0
32	38.51591424089245	41.0	35.0	41.0	35.0	41.0
33	38.419378930290556	40.0	35.0	41.0	35.0	41.0
34	38.32043684048216	40.0	35.0	41.0	35.0	41.0
35	38.24569930679395	40.0	35.0	41.0	35.0	41.0
36	38.18620292031483	40.0	35.0	41.0	35.0	41.0
37	38.1558104611094	40.0	35.0	41.0	35.0	41.0
38	38.06030490339363	40.0	35.0	41.0	35.0	41.0
39	37.992514179214545	40.0	35.0	41.0	35.0	41.0
40	37.92349660101098	40.0	35.0	41.0	35.0	41.0
41	37.88736407395986	40.0	35.0	41.0	35.0	41.0
42	37.83434654938925	40.0	35.0	41.0	35.0	41.0
43	37.773774822005606	40.0	35.0	41.0	35.0	41.0
44	37.656657861921936	40.0	35.0	41.0	35.0	41.0
45	37.649778093616334	40.0	35.0	41.0	35.0	41.0
46	37.57563320416728	39.0	35.0	41.0	35.0	41.0
47	37.47587455249997	39.0	35.0	41.0	35.0	41.0
48	37.400332524369475	39.0	35.0	41.0	35.0	41.0
49	37.35446293291857	39.0	35.0	41.0	35.0	41.0
50	37.28733859830252	39.0	35.0	41.0	35.0	41.0
51	37.21014199326906	39.0	35.0	41.0	34.0	41.0
52	36.74898164411847	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	7.0
14	15.0
15	12.0
16	21.0
17	35.0
18	79.0
19	107.0
20	173.0
21	286.0
22	427.0
23	712.0
24	1071.0
25	1847.0
26	2778.0
27	3208.0
28	3229.0
29	3409.0
30	4047.0
31	4773.0
32	6500.0
33	9298.0
34	35340.0
35	128050.0
36	28441.0
37	45299.0
38	80865.0
39	383638.0
40	2139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.0399029243373	21.846046580228208	22.582695324546467	21.531355170888027
2	28.3014440675239	24.37685201324734	25.32830077365549	21.99340314557327
3	29.174052372588193	24.45435164452072	23.865059465547525	22.50653651734356
4	26.80133009747791	26.291414703476757	22.070098282404366	24.837156916640968
5	24.37792467250372	35.425778683578926	20.63796409273139	19.55833255118596
6	85.69447982730186	3.373379278904815	7.620037274909159	3.3121036188841666
7	85.76152103082555	3.2725493088051913	6.0113165551547985	4.954613105214465
8	74.03789168823158	6.357115082929969	11.737037583298696	7.867955645539748
9	48.661187165632	19.361097330419273	16.32091283302718	15.656802670921548
10	38.814845604108285	21.21800458561832	24.381544897494003	15.585604912779393
11	37.42870168005256	17.941030557380564	24.870543435995764	19.75972432657111
12	23.831941111006824	26.61616229334549	28.424799882007484	21.127096713640203
13	17.342218527507004	29.690671886941715	30.574006784569796	22.39310280098148
14	17.321838001635808	22.960137300384815	38.702618629409635	21.015406068569742
15	20.461377562650004	18.640270310132607	39.925718346495756	20.97263378072163
16	20.306244217696197	22.00614097424277	27.853742910392725	29.833871897668306
17	21.611000120674166	23.298695378179428	30.931872728979233	24.158431772167173
18	24.387042276182942	27.230930129657686	26.859924109357614	21.522103484801757
19	27.692039527493595	23.364529840039687	25.109612367761226	23.83381826470549
20	23.065257907509956	29.25879245384213	26.133733792789048	21.542215845858863
21	21.506281760770168	24.079859481637413	26.307504592322438	28.106354165269977
22	21.35879111301806	27.393438006999098	25.01508427079283	26.232686609190008
23	22.178839114519782	21.333985867714297	33.94363175607729	22.543543261688633
24	22.129764953540445	21.886941714377656	30.243895898419172	25.73939743366273
25	19.48619621619447	26.8196993872434	28.657835105455813	25.036269291106315
26	19.203952749359757	26.05770906799319	28.781593167160537	25.956745015486515
27	20.940856250251404	25.756425899357744	32.32686609190008	20.97585175849077
28	20.569984312358375	25.32964159772596	28.59025757230394	25.510116517611724
29	22.816266877622986	25.220900765610544	28.581542215845857	23.381290140920612
30	26.781754066048997	23.60721899679543	24.795055040828093	24.815971896327483
31	27.909655274131477	25.58238693501026	24.52501307303469	21.982944717823575
32	27.176224507582358	24.28420106997761	26.472694117804803	22.066880304635227
33	20.294713130690123	24.1876617369035	29.560880116919858	25.956745015486515
34	24.13000630187313	23.497405505423632	29.783993242246687	22.58859495045655
35	28.249554175996565	24.804172644507315	24.766897735348145	22.179375444147972
36	25.512395918531528	26.328555530228883	26.17047237232003	21.988576178919566
37	25.307786165377237	26.678108365401375	25.54149180086081	22.472613668360573
38	24.760461779809873	25.200922486960486	25.04297341145868	24.995642321770962
39	28.040385621002667	20.785991070111688	28.134377388342873	23.03924592054277
40	20.032313860098416	20.11986967190035	31.617838323433585	28.229978144567653
41	21.90651774580657	24.384494710449044	26.103297086389293	27.60569045735509
42	22.22107507273971	21.627894503962136	28.85949504565506	27.2915353776431
43	23.378340327965567	25.053700004022474	27.912605087086522	23.655354580925437
44	21.61542484010673	25.359005644869338	27.776913691154583	25.24865582386935
45	22.657781472492992	25.133479036215654	26.692991512583635	25.515747978707715
46	24.264356873734595	21.642643568737345	28.639599898097373	25.453399659430687
47	22.916292353280326	19.596948284415603	32.200158217240315	25.28660114506376
48	22.086456336064145	19.61022244271329	29.41124415065499	28.892077070567574
49	21.432134189672972	22.730320054705622	30.81012590338022	25.027419852241188
50	21.0226465185503	23.32269612904091	29.22004263820544	26.43461471420335
51	21.89646156527802	20.132339335755756	30.12442847373996	27.846770625226263
52	23.111114090720157	19.218165484506777	32.85635751733015	24.814362907442913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	309.5
2	524.0
3	1934.0
4	3344.0
5	2219.5
6	1095.0
7	1087.0
8	1079.0
9	1088.0
10	1097.0
11	1068.5
12	1040.0
13	1037.0
14	972.5
15	911.0
16	876.5
17	842.0
18	887.5
19	933.0
20	933.0
21	933.0
22	1061.5
23	1190.0
24	1608.0
25	2026.0
26	2606.0
27	3186.0
28	4147.5
29	5109.0
30	6030.0
31	6951.0
32	7411.0
33	7871.0
34	9127.0
35	10383.0
36	11235.5
37	12088.0
38	13717.0
39	17956.5
40	20567.0
41	25883.0
42	31199.0
43	38637.5
44	46076.0
45	53298.0
46	60520.0
47	67538.0
48	74556.0
49	77449.5
50	80343.0
51	74179.0
52	68015.0
53	61074.5
54	54134.0
55	49076.0
56	44018.0
57	40559.0
58	37100.0
59	36326.0
60	35552.0
61	34798.5
62	34045.0
63	31625.5
64	23549.5
65	17893.0
66	14992.0
67	12091.0
68	10022.5
69	7954.0
70	6819.5
71	5685.0
72	4881.0
73	4077.0
74	3184.5
75	2292.0
76	1982.0
77	1672.0
78	1298.5
79	925.0
80	652.5
81	380.0
82	278.0
83	176.0
84	190.5
85	205.0
86	120.5
87	36.0
88	22.5
89	5.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	745810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.42582798639368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2279803472804	19.534863368227416
2	10.72033068661969	5.880278907369785
3	3.5910463754586486	2.954622605534741
4	1.7889513974533395	1.962538932102955
5	1.1065463485356963	1.5173974906956016
6	0.7691802973596552	1.2657243915545426
7	0.6594712727804629	1.2660581982471557
8	0.5746531676992404	1.2608271143324485
9	0.5446045163237793	1.3442606806778188
>10	8.676547448710055	51.75769066105407
>50	0.25821608440384275	4.657151994692503
>100	0.07216305020328558	3.6201948479124098
>500	0.007363576551355671	1.3250755957140072
>1k	0.0029454306205422684	1.6533152118845769
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	2910	0.39017980450785056	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2252	0.3019535806706802	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	2095	0.28090264276424287	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT	2069	0.27741650018101127	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1607	0.2154704281251257	No Hit
TGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTTG	1347	0.18060900229280918	Illumina Single End Adapter 1 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	986	0.1322052533487081	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	774	0.1037797830546654	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2851932797897588	0.0
2	0.0	0.0	0.0	1.133532669178477	0.0
3	0.0	0.0	0.0	1.770155937839396	0.0
4	0.0	0.0	0.0	2.1836660811734894	0.0
5	0.0	0.0	0.0	4.502353146243681	0.0
6	0.0	0.0	0.0	4.948847561711428	0.0
7	0.0	0.0	0.0	6.874002762097585	0.0
8	0.0	0.0	0.0	8.897842614070608	0.0
9	0.0	0.0	0.0	10.790281707137206	0.0
10	0.0	0.0	0.0	11.782491519287754	0.0
11	0.0	0.0	0.0	12.707526045507569	0.0
12	0.0	0.0	0.0	13.513227229455223	0.0
13	0.0	0.0	0.0	13.869618267387136	0.0
14	0.0	0.0	0.0	14.12598382966171	0.0
15	0.0	0.0	0.0	14.981161421809844	0.0
16	0.0	0.0	0.0	15.819310548262962	0.0
17	0.0	0.0	0.0	16.849063434386775	0.0
18	0.0	0.0	0.0	17.407114412517934	0.0
19	0.0	0.0	0.0	18.03770397286172	0.0
20	0.0	0.0	0.0	18.767782679234656	0.0
21	0.0	0.0	0.0	19.44650782370845	0.0
22	0.0	0.0	0.0	20.121612743191967	0.0
23	0.0	0.0	0.0	20.646143119561284	0.0
24	0.0	0.0	0.0	21.12374465346402	0.0
25	0.0	0.0	0.0	21.5839154744506	0.0
26	0.0	0.0	0.0	22.023571687158928	0.0
27	1.340824070473713E-4	0.0	0.0	22.447272093428623	0.0
28	1.340824070473713E-4	0.0	0.0	22.881565009855056	0.0
29	1.340824070473713E-4	0.0	0.0	23.41588340193883	0.0
30	1.340824070473713E-4	0.0	0.0	23.914938120969147	0.0
31	1.340824070473713E-4	0.0	0.0	24.382349391936284	0.0
32	1.340824070473713E-4	0.0	0.0	24.86048725546721	0.0
33	1.340824070473713E-4	0.0	0.0	25.293573430230218	0.0
34	1.340824070473713E-4	0.0	0.0	25.820651372333437	0.0
35	1.340824070473713E-4	0.0	0.0	26.25802818412196	0.0
36	1.340824070473713E-4	0.0	0.0	26.70854507180113	0.0
37	1.340824070473713E-4	0.0	0.0	27.18587844088977	0.0
38	1.340824070473713E-4	0.0	0.0	27.680910687708664	0.0
39	1.340824070473713E-4	0.0	0.0	28.21053619554578	0.0
40	1.340824070473713E-4	0.0	0.0	28.731312264517772	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGG	20	6.310023E-4	46.0	1
TACCAGG	60	0.0	46.0	1
CGCATGT	20	6.310023E-4	46.0	39
ATTACGG	30	1.8605188E-6	46.0	1
TCGATCA	45	3.092282E-10	46.0	16
AATGCGG	80	0.0	46.0	1
CCGTAGG	50	1.6370905E-11	46.0	1
ACTACGG	45	3.092282E-10	46.0	1
AACTCCG	20	6.310023E-4	46.0	13
ATTGCGG	130	0.0	46.0	1
CATGCGG	140	0.0	44.35714	1
TTGCGGG	425	0.0	43.835297	2
ATAGCGG	80	0.0	43.125	1
GCATAGG	160	0.0	43.125	1
CTTGCGG	220	0.0	42.863636	1
CGTTTTT	1000	0.0	42.55	1
TACACGG	60	1.8189894E-12	42.166668	1
CCTCGAT	55	4.7293724E-11	41.81818	14
CCATAGG	55	4.7293724E-11	41.81818	1
GCCTACG	55	4.7293724E-11	41.81818	25
>>END_MODULE