FastQCFastQC Report
Thu 26 May 2016
SRR1544930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544930_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2023807
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247711.223980349904907No Hit
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC103340.5106218132460258Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC88880.43917231237958954No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT77440.3826451830634047Illumina PCR Primer Index 8 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT74690.36905693082393726Illumina PCR Primer Index 8 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT73560.36347339444917426Illumina PCR Primer Index 8 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT67140.3317510019483083No Hit
CGTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG42930.21212497041466896Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT39150.19344730006369185Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT37420.18489905410940866No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG24660.12184956371827946No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC23550.11636485099616711No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCTA206.312598E-446.013
TCGACGG1000.046.01
TCGACCG301.8621104E-646.014
CGTTTTT159050.045.4360241
CTTGCGG4050.043.1604921
CGTTTTC8200.042.353661
CGTTCTG8450.042.189351
AGTACGG1200.042.1666681
GCGATCG508.731149E-1041.48
CGTTTCT10400.040.9134641
CGCACTT3500.040.7428634
GCGATAG2500.040.488
TACGGGA3850.040.0259743
AATGCGG2300.040.01
ATTGCGG5250.039.8666651
ACGGGAT4050.039.7530864
CGTAGGG6250.039.7442
CTAGCGG4000.039.6750031
TAGACGG1400.039.4285741
GGACCGA4850.039.3608257
Produced by FastQC (version 0.11.5)