##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544930_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2023807
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78137885677834	33.0	31.0	34.0	31.0	34.0
2	33.12835265418096	34.0	33.0	34.0	31.0	34.0
3	33.125379050472695	34.0	33.0	34.0	31.0	34.0
4	36.509335129288516	37.0	37.0	37.0	35.0	37.0
5	36.499118245959224	37.0	37.0	37.0	35.0	37.0
6	36.59886688799871	37.0	37.0	37.0	35.0	37.0
7	36.40632777730288	37.0	37.0	37.0	35.0	37.0
8	36.450471808823664	37.0	37.0	37.0	35.0	37.0
9	38.25091473643485	39.0	39.0	39.0	37.0	39.0
10	38.164402040313135	39.0	39.0	39.0	35.0	39.0
11	37.93415528259364	39.0	39.0	39.0	35.0	39.0
12	37.91908022850005	39.0	39.0	39.0	35.0	39.0
13	37.92277079780829	39.0	39.0	39.0	35.0	39.0
14	39.422873821466176	41.0	39.0	41.0	35.0	41.0
15	39.46090956301663	41.0	40.0	41.0	35.0	41.0
16	39.457111275927005	41.0	40.0	41.0	35.0	41.0
17	39.447341075507694	41.0	39.0	41.0	35.0	41.0
18	39.323749744911446	41.0	39.0	41.0	35.0	41.0
19	39.178346551820404	41.0	38.0	41.0	35.0	41.0
20	39.01060822499379	41.0	38.0	41.0	35.0	41.0
21	38.983243955574814	41.0	38.0	41.0	35.0	41.0
22	38.94336663525721	41.0	37.0	41.0	35.0	41.0
23	38.91593813046402	41.0	37.0	41.0	35.0	41.0
24	38.84585536071374	41.0	37.0	41.0	35.0	41.0
25	38.8310525657832	41.0	37.0	41.0	35.0	41.0
26	38.770076395624685	41.0	37.0	41.0	35.0	41.0
27	38.69706893987421	41.0	37.0	41.0	35.0	41.0
28	38.67631498458104	41.0	37.0	41.0	35.0	41.0
29	38.616680345507255	41.0	37.0	41.0	35.0	41.0
30	38.52000264847389	41.0	37.0	41.0	35.0	41.0
31	38.33686660832777	41.0	37.0	41.0	35.0	41.0
32	38.0911277607005	41.0	36.0	41.0	35.0	41.0
33	37.77432433033387	40.0	36.0	41.0	34.0	41.0
34	37.49855297466606	40.0	36.0	41.0	33.0	41.0
35	37.34775746896814	40.0	36.0	41.0	33.0	41.0
36	37.24774941484045	40.0	36.0	41.0	33.0	41.0
37	37.18493957180699	40.0	36.0	41.0	33.0	41.0
38	37.09314178674152	40.0	36.0	41.0	32.0	41.0
39	37.0622821247283	40.0	35.0	41.0	32.0	41.0
40	36.96799744244387	40.0	35.0	41.0	32.0	41.0
41	36.88939805030816	40.0	35.0	41.0	31.0	41.0
42	36.79502393261808	40.0	35.0	41.0	31.0	41.0
43	36.679054376232514	40.0	35.0	41.0	31.0	41.0
44	36.608657347266806	40.0	35.0	41.0	31.0	41.0
45	36.53917246061507	40.0	35.0	41.0	31.0	41.0
46	36.558727685001585	40.0	35.0	41.0	31.0	41.0
47	36.47837763185917	40.0	35.0	41.0	31.0	41.0
48	36.4058124119543	40.0	35.0	41.0	31.0	41.0
49	36.36286513486711	40.0	35.0	41.0	31.0	41.0
50	36.28662861626628	39.0	35.0	41.0	30.0	41.0
51	36.1946722192383	39.0	35.0	41.0	30.0	41.0
52	35.99853592758598	39.0	35.0	41.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	1.0
13	9.0
14	12.0
15	25.0
16	46.0
17	104.0
18	221.0
19	376.0
20	743.0
21	1187.0
22	2112.0
23	4427.0
24	9692.0
25	19660.0
26	28152.0
27	29241.0
28	26275.0
29	23413.0
30	22529.0
31	23391.0
32	26306.0
33	32612.0
34	70520.0
35	175670.0
36	80495.0
37	116296.0
38	210051.0
39	1110117.0
40	10121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.66093456540075	22.17148176678903	25.136784288225112	24.03079937958511
2	31.991588130686377	21.40722904901505	24.499964670544177	22.1012181497544
3	22.105813449602653	23.303902002513084	32.14580244064775	22.44448210723651
4	23.29718199413284	25.70067205024985	30.415400282734474	20.586745672882838
5	22.159919399428897	33.025728243849336	26.652986179018058	18.1613661777037
6	80.0106927192168	2.4952478176031607	14.183664746687802	3.310394716492235
7	79.49295560297993	2.2946852145486205	12.280321196635844	5.932037985835606
8	68.15373205053645	4.960996774890096	18.660623270894902	8.224647903678562
9	39.23284186683809	17.22560501075448	24.95119346854715	18.590359653860276
10	28.6939910772124	23.230377204940986	33.75430562301642	14.321326094830189
11	27.549613179517614	17.38046167445809	36.28028759659394	18.789637549430356
12	22.939292136058427	19.556459682173248	36.43721955700322	21.067028624765108
13	16.77852680616284	21.391713735548894	36.08916265236755	25.740596805920724
14	15.535671138601655	21.943495600123924	39.025411019924334	23.495422241350088
15	18.238349803118577	20.18581811407906	38.92816854571607	22.647663537086295
16	19.5452431975974	24.272522033968656	34.42324292780883	21.758991840625118
17	19.87422713727149	23.347977351595286	35.55551492805391	21.222280583079314
18	20.957186134843887	28.190089272346626	32.09955297120723	18.753171621602256
19	20.473839649729445	24.4299975244675	32.21418840828201	22.88197441752104
20	19.09579322534214	29.246909413792917	30.227635342698196	21.429662018166752
21	18.43708416859908	25.173694922490135	31.390295616133358	24.998925292777425
22	17.75223625572992	27.41061771206444	29.857886646305698	24.97925938589994
23	17.3416733907927	23.823912062760925	35.41212180805778	23.42229273838859
24	19.012682533462925	23.93839926435673	33.067876531704854	23.981041670475495
25	18.180735613623238	24.459298737478424	34.316463971119774	23.043501677778565
26	19.385840645871863	23.888246260636514	31.757919603993862	24.967993489497765
27	21.91928380522451	24.65398133320025	33.28869798355278	20.138036878022458
28	22.235074787269735	24.442548128354137	29.532262710821733	23.790114373554395
29	22.48707510152895	26.71509684470901	29.046544458043677	21.751283595718366
30	25.614646060617442	24.022152309978175	26.892287653911662	23.47091397549272
31	25.954451190256776	26.0496677795857	26.2770610043349	21.718820025822623
32	26.05742543631878	26.734960398891793	25.689010859237072	21.518603305552357
33	25.391255193800593	27.18263154539934	26.56216724223209	20.863946018567976
34	27.407504767005946	26.232590360642092	26.260063336078982	20.099841536272976
35	28.97751613666718	26.720087439167866	25.442099963089365	18.86029646107559
36	24.28240439923372	29.14101986997772	24.899508698210848	21.67706703257771
37	23.87579448040253	29.497180314130745	27.098878499777896	19.528146705688833
38	22.5016021784686	28.097787980770896	25.888239342980828	23.51237049777968
39	25.037960635574443	25.08934893495279	27.558507308256175	22.314183121216598
40	19.88756833037933	23.547304658991692	30.51792982235954	26.047197188269433
41	22.49280687338269	24.754386164293336	27.857350033871807	24.89545692845217
42	22.380493792145202	22.915080341159015	30.19838354151359	24.506042325182193
43	23.753055503810394	25.35997750773666	29.002765579919433	21.88420140853352
44	20.640703387230104	26.34361873439513	30.79666193466076	22.219015943714002
45	22.337554915068484	26.30403986150853	29.06621036492116	22.292194858501823
46	23.264916071542395	23.294167872726995	29.35536837257703	24.08554768315358
47	22.38281614798249	21.873676689526224	30.77655132134635	24.96695584114493
48	21.58269044429632	21.76956597145874	28.194338689410603	28.45340489483434
49	21.75647183748253	22.1825500158859	31.466587475979672	24.594390670651894
50	21.321548942166917	22.19633591543067	31.677328915257235	24.80478622714518
51	20.304604144565168	21.158391091640656	32.59693241499807	25.940072348796107
52	18.852489392516183	20.79852476051323	35.40263473740332	24.946351109567267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	568.0
2	965.0
3	23272.5
4	45580.0
5	30169.5
6	14759.0
7	14499.0
8	14239.0
9	14041.5
10	13844.0
11	13400.0
12	12956.0
13	12273.0
14	10759.5
15	9929.0
16	9113.0
17	8297.0
18	7555.0
19	6813.0
20	6265.5
21	5718.0
22	5469.5
23	5221.0
24	5832.0
25	6443.0
26	7089.0
27	7735.0
28	9899.5
29	12064.0
30	13921.0
31	15778.0
32	18190.0
33	20602.0
34	25775.0
35	30948.0
36	35231.0
37	39514.0
38	44408.5
39	54717.5
40	60132.0
41	67800.0
42	75468.0
43	86995.0
44	98522.0
45	111019.5
46	123517.0
47	149836.0
48	176155.0
49	210621.0
50	245087.0
51	248979.5
52	252872.0
53	231745.5
54	210619.0
55	173839.5
56	137060.0
57	111888.5
58	86717.0
59	74290.0
60	61863.0
61	55783.5
62	49704.0
63	43858.0
64	32504.0
65	26996.0
66	21934.5
67	16873.0
68	14229.5
69	11586.0
70	9493.5
71	7401.0
72	6135.5
73	4870.0
74	4229.0
75	3588.0
76	2903.5
77	2219.0
78	1656.0
79	1093.0
80	793.0
81	493.0
82	395.5
83	298.0
84	226.0
85	154.0
86	93.5
87	33.0
88	18.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2023807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.65793744194299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.42761049716012	26.53935079061035
2	9.910082110518188	7.067461758827519
3	3.8430597558679844	4.111067530811678
4	2.201081317920058	3.1394407975609147
5	1.4738409515255626	2.627706422443612
6	1.1212975353067647	2.3989894420664086
7	0.9006796969670163	2.2481466202774625
8	0.7250679132328072	2.068354103297247
9	0.6107445553546628	1.960010203305947
>10	4.472803672124033	29.773477114542512
>50	0.19088740193409542	4.65682475713704
>100	0.11517594692444083	7.062589918594951
>500	0.0046011870986918035	1.1593700970175556
>1k	0.0020914486812235473	1.5440866719445185
>5k	6.971495604078491E-4	1.8977793204364304
>10k+	2.7885982416313965E-4	1.745344451125747
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24771	1.223980349904907	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	10334	0.5106218132460258	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	8888	0.43917231237958954	No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	7744	0.3826451830634047	Illumina PCR Primer Index 8 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT	7469	0.36905693082393726	Illumina PCR Primer Index 8 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT	7356	0.36347339444917426	Illumina PCR Primer Index 8 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT	6714	0.3317510019483083	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	4293	0.21212497041466896	Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	3915	0.19344730006369185	Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT	3742	0.18489905410940866	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG	2466	0.12184956371827946	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC	2355	0.11636485099616711	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3708851683979747	0.0
2	0.0	0.0	0.0	1.1094437364827772	0.0
3	0.0	0.0	0.0	2.2082639303056073	0.0
4	0.0	0.0	0.0	2.779118759842218	0.0
5	0.0	0.0	0.0	5.290227773695812	0.0
6	0.0	0.0	0.0	6.69367187681434	0.0
7	4.941182632533636E-5	0.0	0.0	9.998137174147535	0.0
8	4.941182632533636E-5	0.0	0.0	13.422376738493345	0.0
9	4.941182632533636E-5	0.0	0.0	16.923303457296075	0.0
10	9.882365265067272E-5	0.0	0.0	18.430265336566183	0.0
11	9.882365265067272E-5	0.0	0.0	19.66486922913104	0.0
12	1.482354789760091E-4	0.0	0.0	20.535604432636116	0.0
13	1.9764730530134544E-4	0.0	0.0	21.02280504020393	0.0
14	1.9764730530134544E-4	0.0	0.0	21.363548994543454	0.0
15	1.9764730530134544E-4	0.0	0.0	22.098401675653854	0.0
16	1.9764730530134544E-4	0.0	0.0	22.874809702703864	0.0
17	1.9764730530134544E-4	0.0	0.0	23.81813087908086	0.0
18	1.9764730530134544E-4	0.0	0.0	24.328555045021584	0.0
19	1.9764730530134544E-4	0.0	0.0	24.957814653274745	0.0
20	1.9764730530134544E-4	0.0	0.0	25.64834492617132	0.0
21	1.9764730530134544E-4	0.0	0.0	26.355131689928932	0.0
22	1.9764730530134544E-4	0.0	0.0	27.055494916264248	0.0
23	1.9764730530134544E-4	0.0	0.0	27.623730919005617	0.0
24	1.9764730530134544E-4	0.0	0.0	28.165679830141904	0.0
25	1.9764730530134544E-4	0.0	0.0	28.697993435144756	0.0
26	1.9764730530134544E-4	0.0	0.0	29.294838885328492	0.0
27	2.4705913162668177E-4	0.0	0.0	29.839208975954723	0.0
28	2.4705913162668177E-4	0.0	0.0	30.387383777208004	0.0
29	2.4705913162668177E-4	0.0	0.0	31.07485051687241	0.0
30	2.964709579520182E-4	0.0	0.0	31.651931236526014	0.0
31	3.4588278427735453E-4	0.0	0.0	32.193731912183324	0.0
32	3.4588278427735453E-4	0.0	0.0	32.75341966897041	0.0
33	3.4588278427735453E-4	0.0	0.0	33.30678271198785	0.0
34	3.4588278427735453E-4	0.0	0.0	33.93021172473462	0.0
35	3.4588278427735453E-4	0.0	0.0	34.452445317167104	0.0
36	3.952946106026909E-4	0.0	0.0	34.97868126753193	0.0
37	3.952946106026909E-4	0.0	0.0	35.52744901070112	0.0
38	3.952946106026909E-4	0.0	0.0	36.07774852048639	0.0
39	3.952946106026909E-4	0.0	0.0	36.59864799360808	0.0
40	3.952946106026909E-4	0.0	0.0	37.1029945049108	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCTA	20	6.312598E-4	46.0	13
TCGACGG	100	0.0	46.0	1
TCGACCG	30	1.8621104E-6	46.0	14
CGTTTTT	15905	0.0	45.436024	1
CTTGCGG	405	0.0	43.160492	1
CGTTTTC	820	0.0	42.35366	1
CGTTCTG	845	0.0	42.18935	1
AGTACGG	120	0.0	42.166668	1
GCGATCG	50	8.731149E-10	41.4	8
CGTTTCT	1040	0.0	40.913464	1
CGCACTT	350	0.0	40.74286	34
GCGATAG	250	0.0	40.48	8
TACGGGA	385	0.0	40.025974	3
AATGCGG	230	0.0	40.0	1
ATTGCGG	525	0.0	39.866665	1
ACGGGAT	405	0.0	39.753086	4
CGTAGGG	625	0.0	39.744	2
CTAGCGG	400	0.0	39.675003	1
TAGACGG	140	0.0	39.428574	1
GGACCGA	485	0.0	39.360825	7
>>END_MODULE