FastQCFastQC Report
Thu 26 May 2016
SRR1544929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544929_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1319367
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159341.2077003593389861No Hit
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC80990.6138549774247802Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT70500.5343471528392024Illumina PCR Primer Index 8 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT61610.46696635583579094Illumina PCR Primer Index 8 (96% over 25bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC52530.39814547430699726No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT51680.3917029909039714Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT35780.27119065430619377No Hit
CGTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG29420.22298571966708278Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT29000.21980237492676413Illumina PCR Primer Index 8 (95% over 24bp)
TGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTTG24830.1881963092907432Illumina PCR Primer Index 8 (96% over 26bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT18590.14090090172029468No Hit
ACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC15140.11475199849624858Illumina PCR Primer Index 8 (95% over 23bp)
GTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTTGA13910.10542934604245824Illumina PCR Primer Index 8 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG13880.10520196427529262No Hit
GCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC13440.10186703169019688Illumina PCR Primer Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC405.6115823E-946.024
ACAACGG850.046.01
CGTTTTT105850.045.5002371
CGTTCTG5550.045.171171
AATGCGG1650.044.606061
CATGCGG2350.043.063831
ATAACGG650.042.461541
TACGCGG1150.042.01
CGTTTTC4600.042.01
CACGACG451.5887053E-840.8888925
CACGAGG1600.040.251
AAGTGCG402.9110197E-740.2511
AACACGG800.040.251
CGTTTCT6450.039.9379841
TATCCGG750.039.8666651
ATTACGG1100.039.7272721
ATAGCGG2150.039.5813941
CTTAATG12750.039.50588246
TTATCGG355.373715E-639.4285741
CCGTAGG700.039.4285741
Produced by FastQC (version 0.11.5)