FastQCFastQC Report
Thu 26 May 2016
SRR1544928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544928_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences785181
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT40030.5098187551660063TruSeq Adapter, Index 16 (100% over 24bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT31710.40385592621319155TruSeq Adapter, Index 16 (100% over 24bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT30320.3861530016645843TruSeq Adapter, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT14170.18046794306026254TruSeq Adapter, Index 16 (100% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC11650.14837343236782347TruSeq Adapter, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC11220.14289698808300252TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.12926955695565734No Hit
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC10030.12774124692268407TruSeq Adapter, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC9330.11882610506367322TruSeq Adapter, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT8210.10456187808925586TruSeq Adapter, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG750.046.0000041
GCATAAA206.3102273E-446.013
ACAACGA206.3102273E-446.012
GTCATCG206.3102273E-446.043
AGGTATT253.4161596E-546.05
AGCGTCG253.4161596E-546.026
AGTCCCG206.3102273E-446.020
CAAACGG900.046.01
GTACAAA253.4161596E-546.08
TTTACGG206.3102273E-446.01
CACGGGT206.3102273E-446.03
CGTGCAC206.3102273E-446.031
ACGCAAC206.3102273E-446.026
GCGCGAA206.3102273E-446.030
TGGGTAC301.8606443E-646.05
CGTTGAT253.4161596E-546.024
CGGCAGT600.046.011
TAGCGCA206.3102273E-446.025
ATAGCGT206.3102273E-446.09
GTCTCCG206.3102273E-446.08
Produced by FastQC (version 0.11.5)