##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544928_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	785181
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.878419116101895	34.0	33.0	34.0	31.0	34.0
2	33.159885427691194	34.0	33.0	34.0	31.0	34.0
3	33.210354045755054	34.0	33.0	34.0	31.0	34.0
4	36.5159956748826	37.0	37.0	37.0	35.0	37.0
5	36.49734774529694	37.0	37.0	37.0	35.0	37.0
6	36.62844108555861	37.0	37.0	37.0	35.0	37.0
7	36.4453317133247	37.0	37.0	37.0	35.0	37.0
8	36.49479546754188	37.0	37.0	37.0	35.0	37.0
9	38.268366147423336	39.0	39.0	39.0	37.0	39.0
10	38.05073861950302	39.0	39.0	39.0	35.0	39.0
11	37.07226104554236	39.0	35.0	39.0	35.0	39.0
12	36.919981507448604	39.0	35.0	39.0	35.0	39.0
13	36.89251013460591	39.0	35.0	39.0	35.0	39.0
14	38.11318027308353	40.0	35.0	41.0	35.0	41.0
15	38.203986087284335	40.0	35.0	41.0	35.0	41.0
16	38.232308219378716	40.0	35.0	41.0	35.0	41.0
17	38.34513188678789	40.0	36.0	41.0	35.0	41.0
18	38.31925887152134	40.0	36.0	41.0	35.0	41.0
19	38.18775925550924	40.0	35.0	41.0	35.0	41.0
20	38.078057161342414	40.0	35.0	41.0	35.0	41.0
21	38.02616976213128	40.0	35.0	41.0	35.0	41.0
22	38.00307954471644	40.0	35.0	41.0	35.0	41.0
23	37.99262080972413	40.0	35.0	41.0	35.0	41.0
24	37.945867258632084	40.0	35.0	41.0	35.0	41.0
25	37.91273222352553	40.0	35.0	41.0	35.0	41.0
26	37.85737301335615	40.0	35.0	41.0	35.0	41.0
27	37.85175010602651	40.0	35.0	41.0	35.0	41.0
28	37.87761165896781	40.0	35.0	41.0	35.0	41.0
29	37.88661213147032	40.0	35.0	41.0	35.0	41.0
30	37.79529305981678	40.0	35.0	41.0	35.0	41.0
31	37.680971648575294	40.0	35.0	41.0	35.0	41.0
32	37.64168771276941	40.0	35.0	41.0	35.0	41.0
33	37.536518331442046	39.0	35.0	41.0	35.0	41.0
34	37.468239807127276	39.0	35.0	41.0	35.0	41.0
35	37.39092260255916	39.0	35.0	41.0	35.0	41.0
36	37.36437967806149	39.0	35.0	41.0	34.0	41.0
37	37.313327755001716	39.0	35.0	41.0	34.0	41.0
38	37.31207199359129	39.0	35.0	41.0	35.0	41.0
39	37.20772917327342	39.0	35.0	41.0	34.0	41.0
40	37.17304417707509	39.0	35.0	41.0	34.0	41.0
41	37.121649657849595	38.0	35.0	41.0	34.0	41.0
42	36.9983685290398	38.0	35.0	41.0	34.0	41.0
43	36.868949961855925	38.0	35.0	41.0	34.0	41.0
44	36.796891417392935	38.0	35.0	41.0	34.0	41.0
45	36.76847886028826	38.0	35.0	41.0	34.0	41.0
46	36.80167884857122	38.0	35.0	41.0	34.0	41.0
47	36.76574063814586	37.0	35.0	41.0	34.0	41.0
48	36.83782338085104	37.0	35.0	41.0	34.0	41.0
49	36.727740482767665	37.0	35.0	41.0	34.0	41.0
50	36.616990222636566	37.0	35.0	41.0	34.0	41.0
51	36.548427687373994	37.0	35.0	41.0	33.0	41.0
52	36.3778835707945	36.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	5.0
14	13.0
15	26.0
16	19.0
17	40.0
18	58.0
19	141.0
20	227.0
21	415.0
22	644.0
23	1075.0
24	1880.0
25	3025.0
26	3975.0
27	4744.0
28	4826.0
29	5106.0
30	6241.0
31	7535.0
32	9943.0
33	14600.0
34	43526.0
35	211162.0
36	25499.0
37	36732.0
38	67102.0
39	333436.0
40	3180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.9129996777813	21.780965153257657	25.890081395245172	21.41595377371587
2	25.566971182440735	24.387115836985355	27.673491844555585	22.37242113601832
3	25.07383647846802	25.310469815240054	26.890232952656778	22.725460753635147
4	26.296484504846653	27.661392723461216	23.48401196666756	22.558110805024572
5	22.98107060664993	35.470420196107646	21.611704817105863	19.936804380136554
6	86.64218823430521	2.605386528711214	7.63110671297446	3.121318524009114
7	86.19948776142061	2.1815352128999557	5.7418607938806465	5.877116231798783
8	76.28050092908514	3.729713276301897	12.66612411660496	7.323661678008
9	53.31293039439314	16.385903377692532	14.571162572706168	15.730003655208163
10	47.58686213751989	16.866938960570874	24.6899759418529	10.856222960056344
11	43.2954949241003	15.645055089208732	23.525531055896664	17.5339189307943
12	18.118752236745415	38.89421674747606	28.061682592930804	14.925348422847726
13	11.20200310501655	42.71359087904572	28.15019721567384	17.93420880026389
14	9.66108451427123	20.769733348106996	53.226962955038395	16.342219182583378
15	10.14084650545543	15.520752539860236	54.354601041033845	19.983799913650483
16	11.090691190948329	18.973077545177482	29.2240897321764	40.71214153169779
17	16.32120491963	21.306170169680623	38.59059248759203	23.78203242309735
18	24.59687638901094	25.903963544711345	28.125107459299194	21.37405260697852
19	26.52458477726792	20.660713899088236	26.24643235126678	26.56826897237707
20	16.05426010053733	33.314611535429414	28.06461185382733	22.56651651020593
21	18.57240559819965	24.052415939764206	24.26561518936398	33.10956327267216
22	14.666299872258753	33.854614413746646	21.702129827390117	29.77695588660449
23	14.744625761448635	22.560657988412864	40.74092470398545	21.953791546153052
24	15.248840713160405	21.50688822067778	38.78570673513496	24.458564331026857
25	12.728275391279208	31.937222118212233	30.57600731551069	24.758495174997865
26	16.419271480079114	31.49757826539358	25.799146948283262	26.284003306244035
27	16.411247852406007	27.15399379251408	39.25693566196839	17.177822693111526
28	13.468996320593597	24.171624122336123	33.81156701448455	28.54781254258572
29	15.83953254090458	29.06285302369772	27.369103429655073	27.72851100574262
30	25.601485517352053	23.275652365505533	23.46567224627188	27.657189870870535
31	27.18379583815706	24.98430298236967	21.628134149960328	26.203767029512942
32	22.177306888475396	23.99777885608541	24.443153871527713	29.38176038391148
33	13.761795051077394	25.178016279049036	26.785288997059276	34.2748996728143
34	13.573430839513437	22.890772955535095	31.000877504677266	32.534918700274204
35	26.859794111166725	20.01245572676873	25.961784607625503	27.16596555443904
36	17.07071363163398	24.174043946555	25.506730295307705	33.248512126503314
37	16.32591720889833	26.99657785911783	27.214234679647113	29.46327025233672
38	15.433511508811343	26.36551317466928	26.273305136013224	31.92767018050615
39	23.03608976783697	19.326499240302557	27.388589382575486	30.24882160928499
40	13.089975432416221	17.06090697558907	30.410695113610746	39.43842247838397
41	20.084158939149063	22.659361344709055	25.003152139443007	32.25332757669888
42	19.911587264592495	20.538576455619786	29.666662845891583	29.883173433896133
43	21.694615636394666	26.362456554603337	27.600260322142283	24.342667486859717
44	14.405595652467392	31.12441589900927	25.96509594603028	28.504892502493057
45	20.890724559050717	27.537472251620965	26.04278503937309	25.529018149955235
46	21.96461707555328	20.57449174139466	27.52040612291943	29.940485060132634
47	21.1013766252622	17.54245199514507	32.956477551036	28.399693828556728
48	19.409027982083114	15.390336750379848	29.370043340325353	35.830591927211685
49	20.145673417976237	23.28214768314567	29.93411710166191	26.638061797216185
50	18.01622810536679	25.386503239380474	26.556551928790938	30.040716726461795
51	17.54512653770277	19.72475136306151	32.99188340013322	29.7382386991025
52	21.808220015512347	16.93469403869936	35.52645823065001	25.730627715138294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	150.0
1	184.5
2	219.0
3	1037.0
4	1855.0
5	1343.5
6	832.0
7	1008.5
8	1185.0
9	1297.0
10	1409.0
11	1389.5
12	1370.0
13	1333.0
14	1180.5
15	1065.0
16	1000.5
17	936.0
18	902.5
19	869.0
20	880.5
21	892.0
22	956.5
23	1021.0
24	1300.0
25	1579.0
26	1966.0
27	2353.0
28	2758.0
29	3163.0
30	3674.0
31	4185.0
32	4883.5
33	5582.0
34	6865.0
35	8148.0
36	8330.5
37	8513.0
38	10339.0
39	15221.0
40	18277.0
41	30210.0
42	42143.0
43	57532.0
44	72921.0
45	78960.0
46	84999.0
47	85482.5
48	85966.0
49	93548.0
50	101130.0
51	101831.5
52	102533.0
53	88196.0
54	73859.0
55	60147.0
56	46435.0
57	36455.5
58	26476.0
59	23766.5
60	21057.0
61	18857.5
62	16658.0
63	14621.0
64	10249.5
65	7915.0
66	6350.0
67	4785.0
68	3844.0
69	2903.0
70	2521.5
71	2140.0
72	1754.0
73	1368.0
74	1155.0
75	942.0
76	888.5
77	835.0
78	565.0
79	295.0
80	217.5
81	140.0
82	80.0
83	20.0
84	14.5
85	9.0
86	5.0
87	1.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	785181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.907373557323435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.12656196626945	14.924740421712077
2	12.653004216221172	5.5438817997429135
3	5.488628126687398	3.6072428006571946
4	2.919914658798951	2.5587064474325283
5	1.6623752793341315	1.8209138118416357
6	1.043909784410024	1.3721592964329221
7	0.6941384373796234	1.064472503372052
8	0.5238012396266405	0.918008754103192
9	0.39518105531432474	0.7791641101393387
>10	4.098158504103476	22.819698722508566
>50	2.041466323415122	30.249238712270554
>100	0.34296168411052624	11.563859408534663
>500	0.005240501115440978	0.7461089393679111
>1k	0.004658223213725313	2.0318042718844396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	4003	0.5098187551660063	TruSeq Adapter, Index 16 (100% over 24bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	3171	0.40385592621319155	TruSeq Adapter, Index 16 (100% over 24bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT	3032	0.3861530016645843	TruSeq Adapter, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	1417	0.18046794306026254	TruSeq Adapter, Index 16 (100% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1165	0.14837343236782347	TruSeq Adapter, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1122	0.14289698808300252	TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1015	0.12926955695565734	No Hit
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1003	0.12774124692268407	TruSeq Adapter, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	933	0.11882610506367322	TruSeq Adapter, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	821	0.10456187808925586	TruSeq Adapter, Index 16 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39888891860602843	0.0
2	0.0	0.0	0.0	1.631725678537815	0.0
3	0.0	0.0	0.0	2.6183771639914872	0.0
4	0.0	0.0	0.0	3.222314345354765	0.0
5	0.0	0.0	0.0	6.437751295561151	0.0
6	0.0	0.0	0.0	7.08588210871124	0.0
7	0.0	0.0	0.0	10.674608784471351	0.0
8	0.0	0.0	0.0	14.101079878397465	0.0
9	0.0	0.0	0.0	17.827736534633416	0.0
10	0.0	0.0	0.0	19.06133744958169	0.0
11	0.0	0.0	0.0	20.324103614325868	0.0
12	0.0	0.0	0.0	21.440152015904612	0.0
13	0.0	0.0	0.0	21.78567744252599	0.0
14	0.0	0.0	0.0	22.068669516964878	0.0
15	0.0	0.0	0.0	23.15338756286767	0.0
16	0.0	0.0	0.0	24.23466691119627	0.0
17	0.0	0.0	0.0	26.15728093267667	0.0
18	0.0	0.0	0.0	26.731033990888726	0.0
19	0.0	0.0	0.0	27.62624159270283	0.0
20	0.0	0.0	0.0	28.54768518341631	0.0
21	0.0	0.0	0.0	29.237335085795504	0.0
22	1.2735916941444074E-4	0.0	0.0	30.16756645919858	0.0
23	1.2735916941444074E-4	0.0	0.0	30.717503352730134	0.0
24	1.2735916941444074E-4	0.0	0.0	31.14963301455333	0.0
25	1.2735916941444074E-4	0.0	0.0	31.6608527205829	0.0
26	1.2735916941444074E-4	0.0	0.0	32.09272766406727	0.0
27	1.2735916941444074E-4	0.0	0.0	32.58751803724237	0.0
28	1.2735916941444074E-4	0.0	0.0	32.98653431501781	0.0
29	1.2735916941444074E-4	0.0	0.0	33.62982547973015	0.0
30	1.2735916941444074E-4	0.0	0.0	34.23465417527933	0.0
31	1.2735916941444074E-4	0.0	0.0	34.668948942982574	0.0
32	1.2735916941444074E-4	0.0	0.0	35.09445592799622	0.0
33	1.2735916941444074E-4	0.0	0.0	35.5008590375977	0.0
34	1.2735916941444074E-4	0.0	0.0	36.18936270745217	0.0
35	1.2735916941444074E-4	0.0	0.0	36.61041212153631	0.0
36	1.2735916941444074E-4	0.0	0.0	37.05616921448685	0.0
37	1.2735916941444074E-4	0.0	0.0	37.448308097113916	0.0
38	1.2735916941444074E-4	0.0	0.0	37.79205049536349	0.0
39	1.2735916941444074E-4	0.0	0.0	38.24735952602011	0.0
40	1.2735916941444074E-4	0.0	0.0	38.652616403096864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	75	0.0	46.000004	1
GCATAAA	20	6.3102273E-4	46.0	13
ACAACGA	20	6.3102273E-4	46.0	12
GTCATCG	20	6.3102273E-4	46.0	43
AGGTATT	25	3.4161596E-5	46.0	5
AGCGTCG	25	3.4161596E-5	46.0	26
AGTCCCG	20	6.3102273E-4	46.0	20
CAAACGG	90	0.0	46.0	1
GTACAAA	25	3.4161596E-5	46.0	8
TTTACGG	20	6.3102273E-4	46.0	1
CACGGGT	20	6.3102273E-4	46.0	3
CGTGCAC	20	6.3102273E-4	46.0	31
ACGCAAC	20	6.3102273E-4	46.0	26
GCGCGAA	20	6.3102273E-4	46.0	30
TGGGTAC	30	1.8606443E-6	46.0	5
CGTTGAT	25	3.4161596E-5	46.0	24
CGGCAGT	60	0.0	46.0	11
TAGCGCA	20	6.3102273E-4	46.0	25
ATAGCGT	20	6.3102273E-4	46.0	9
GTCTCCG	20	6.3102273E-4	46.0	8
>>END_MODULE