FastQCFastQC Report
Thu 26 May 2016
SRR1544927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544927_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences383824
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT23730.6182521155529618TruSeq Adapter, Index 16 (100% over 24bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT18250.4754783442411105TruSeq Adapter, Index 16 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT14670.38220642794614196TruSeq Adapter, Index 16 (100% over 24bp)
TGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTTG9800.25532535745550045TruSeq Adapter, Index 16 (100% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.16960898745258243No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT6250.16283504939764057TruSeq Adapter, Index 16 (100% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC5870.15293467839426403TruSeq Adapter, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC4970.1294864312810038TruSeq Adapter, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC4930.1284442869648589TruSeq Adapter, Index 16 (100% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA4880.12714160656967777No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4830.12583892617449663No Hit
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC4520.11776230772437365TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCGG351.01719706E-746.0000041
CCACTTA351.01719706E-746.00000425
GTCGGGA351.01719706E-746.0000043
GCCCCGG206.3061755E-446.01
CGGGTAT600.046.05
AGACTAC206.3061755E-446.016
AATCCGG253.4128738E-546.01
CTCACGG453.092282E-1046.01
GGTACAA206.3061755E-446.07
CAAACGG501.6370905E-1146.01
ACCGGGT206.3061755E-446.03
CCGGGAT206.3061755E-446.04
GTCGAGG405.5952114E-946.01
CTCCGGT206.3061755E-446.021
CGAGTCT253.4128738E-546.016
TTTACGG301.8581413E-646.01
ATGGACT253.4128738E-546.039
TGGGTAC301.8581413E-646.05
TAGCGCC253.4128738E-546.020
CCGGATA206.3061755E-446.019
Produced by FastQC (version 0.11.5)