##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544927_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	383824
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.921200862895496	34.0	33.0	34.0	31.0	34.0
2	33.24821011713702	34.0	33.0	34.0	31.0	34.0
3	33.36813747967818	34.0	34.0	34.0	31.0	34.0
4	36.600019800742004	37.0	37.0	37.0	35.0	37.0
5	36.58773552461545	37.0	37.0	37.0	35.0	37.0
6	36.41672485305765	37.0	37.0	37.0	35.0	37.0
7	36.24855923548293	37.0	37.0	37.0	35.0	37.0
8	36.38669806994873	37.0	37.0	37.0	35.0	37.0
9	38.2575972320647	39.0	39.0	39.0	37.0	39.0
10	38.09947788569761	39.0	39.0	39.0	35.0	39.0
11	37.16666492975947	39.0	35.0	39.0	35.0	39.0
12	37.08810548584768	39.0	35.0	39.0	35.0	39.0
13	37.07263224811372	39.0	35.0	39.0	35.0	39.0
14	38.22492861311434	40.0	35.0	41.0	35.0	41.0
15	38.28250187585977	40.0	35.0	41.0	35.0	41.0
16	38.335833611238485	40.0	35.0	41.0	35.0	41.0
17	38.46994716328317	40.0	36.0	41.0	35.0	41.0
18	38.452254158155824	40.0	36.0	41.0	35.0	41.0
19	38.33608112051357	40.0	35.0	41.0	35.0	41.0
20	38.23455802659552	40.0	35.0	41.0	35.0	41.0
21	38.18042644545417	40.0	35.0	41.0	35.0	41.0
22	38.1619544374505	40.0	35.0	41.0	35.0	41.0
23	38.13361071741215	40.0	35.0	41.0	35.0	41.0
24	38.10772124723832	40.0	35.0	41.0	35.0	41.0
25	38.07039163783401	40.0	35.0	41.0	35.0	41.0
26	38.01094251531952	40.0	35.0	41.0	35.0	41.0
27	38.104305619242155	40.0	35.0	41.0	35.0	41.0
28	38.11428415106924	40.0	35.0	41.0	35.0	41.0
29	38.13753439076243	40.0	35.0	41.0	35.0	41.0
30	38.03840822877152	40.0	35.0	41.0	35.0	41.0
31	37.94375547125766	40.0	35.0	41.0	35.0	41.0
32	37.89464963108091	40.0	35.0	41.0	35.0	41.0
33	37.80547334194839	40.0	35.0	41.0	35.0	41.0
34	37.72322470715745	40.0	35.0	41.0	35.0	41.0
35	37.639639522281044	40.0	35.0	41.0	35.0	41.0
36	37.59834976447539	40.0	35.0	41.0	35.0	41.0
37	37.54072439451415	40.0	35.0	41.0	35.0	41.0
38	37.5625547125766	40.0	35.0	41.0	35.0	41.0
39	37.50400704489558	39.0	35.0	41.0	35.0	41.0
40	37.492718016590935	39.0	35.0	41.0	35.0	41.0
41	37.47138532243945	39.0	35.0	41.0	35.0	41.0
42	37.3906504022677	39.0	35.0	41.0	35.0	41.0
43	37.29916055275334	39.0	35.0	41.0	35.0	41.0
44	37.16894201509025	39.0	35.0	41.0	35.0	41.0
45	37.17679196715161	39.0	35.0	41.0	35.0	41.0
46	37.14681208053691	38.0	35.0	41.0	35.0	41.0
47	37.09336570928342	38.0	35.0	41.0	35.0	41.0
48	37.1072132018842	38.0	35.0	41.0	35.0	41.0
49	37.02039215890616	38.0	35.0	41.0	35.0	41.0
50	36.93257326274542	37.0	35.0	41.0	34.0	41.0
51	36.86130361832507	37.0	35.0	41.0	34.0	41.0
52	36.47344355746384	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	6.0
16	10.0
17	20.0
18	21.0
19	63.0
20	83.0
21	151.0
22	258.0
23	427.0
24	711.0
25	1273.0
26	1835.0
27	2104.0
28	1875.0
29	1820.0
30	2152.0
31	2583.0
32	3519.0
33	5554.0
34	19623.0
35	104011.0
36	11236.0
37	17138.0
38	33082.0
39	172603.0
40	1661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.089572303972655	21.3960044186919	25.00885822668723	23.505565050648215
2	25.49371586977365	24.893440743674184	27.36436491725374	22.248478469298426
3	25.086497978240025	25.109946225353287	27.073867189128347	22.729688607278337
4	26.091385635082748	27.576441285589226	23.876047355037723	22.4561257242903
5	23.10902913835508	34.982700404351995	21.81572804201926	20.092542415273666
6	85.87399433073492	2.662939263829255	7.946610946683896	3.5164554587519277
7	85.51966526324566	2.0517216224102715	6.363333194380758	6.0652799199633165
8	75.57995331193463	3.8238880320146733	13.046604693818	7.5495539622326895
9	53.43386552169744	16.007857768143733	14.787767310017092	15.770509400141734
10	47.40975030222185	16.7498645212389	24.78609987911126	11.054285297427988
11	42.86131143440743	15.870555254491642	23.563404060194255	17.704729250906663
12	18.10673642085956	38.55855808912418	27.932073033473674	15.40263245654258
13	11.513610404768853	42.24514360748676	28.28379674017258	17.957449247571805
14	9.725030222185168	20.798074117303763	52.536579265496684	16.94031639501438
15	10.38679186293718	15.5394138980366	53.81086122806287	20.26293301096336
16	11.436231189295093	19.055348284630455	29.37622451957147	40.13219600650298
17	16.556286214514987	21.606778106632206	38.151861269748636	23.685074409104175
18	24.672506148651465	25.78186877318771	27.923214806786444	21.622410271374378
19	26.66690983367377	20.947882362749592	25.73236691817083	26.65284088540581
20	16.293144774688397	33.29494768435533	27.670755346200345	22.74115219475593
21	18.595502105131516	24.2311580307641	24.26216182416941	32.91117803993497
22	15.134019759056233	33.957751469423485	21.801398557672265	29.106830213848013
23	15.241100087540122	22.919619408895745	39.91699320521906	21.922287298345076
24	15.392211013381132	21.787850681562382	38.01143232314811	24.808505981908375
25	13.173485764308642	31.80285755971487	30.449893701279752	24.573762974696738
26	16.67066155321189	30.67082829630247	25.960857059485598	26.69765309100004
27	16.657113677102007	26.553837175372045	39.417285005627576	17.371764141898367
28	13.61457334611697	23.991985910208847	34.15549835341198	28.237942390262205
29	16.149328859060404	28.147015298678564	27.536058193338615	28.16759764892242
30	25.67765434157322	22.821397307099087	23.469089999583144	28.03185835174455
31	27.17026553837175	25.10421443161449	21.165169452665804	26.56035057734795
32	22.104923089749466	23.67960315144441	24.903080578598523	29.312393180207597
33	13.741193880528577	24.868168744007672	27.266924423694196	34.12371295176956
34	14.022051773729627	22.853182708741507	31.35708032848389	31.767685189044983
35	26.717974905164866	19.91615948976614	26.095293676268287	27.270571928800702
36	17.393388636458376	24.203280670307223	25.051846679728207	33.35148401350619
37	16.723550377256245	26.867262078452626	27.234357413814664	29.17483013047647
38	15.898693151027555	26.247186210346406	25.94261953395306	31.911501104672972
39	23.11033181875026	19.455792238109133	27.280732835883114	30.153143107257492
40	13.32876526741423	17.02733544541248	29.90771812080537	39.73618116636792
41	20.48517028638126	23.013412397348784	24.35960231772896	32.141814998540994
42	20.35516278294218	20.929123765058986	28.967443411563632	29.748270040435198
43	21.941566968193754	26.472549918712744	27.563675017716456	24.02220809537705
44	14.698403434907666	30.564529576055694	26.29330109633582	28.443765892700824
45	20.783484096877736	27.346387927800244	26.56373754637542	25.3063904289466
46	22.223727541789987	20.139959981658258	27.76402726249531	29.872285214056443
47	21.418671057568055	17.052607445078994	32.94843469923715	28.5802867981158
48	19.10693442827963	15.410448538913668	29.548959939972484	35.93365709283422
49	19.82184542915503	23.248676476718497	29.949664429530205	26.979813664596275
50	18.061663679186292	25.75425194880987	26.633040143399057	29.551044228604777
51	17.887886114469133	19.60846638042436	32.720726166159494	29.782921338947016
52	22.430332652465715	17.22351911292676	34.509827420901246	25.83632081370628
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	96.5
2	125.0
3	622.0
4	1119.0
5	830.0
6	541.0
7	585.0
8	629.0
9	705.0
10	781.0
11	781.0
12	781.0
13	754.5
14	675.0
15	622.0
16	563.5
17	505.0
18	482.0
19	459.0
20	468.0
21	477.0
22	494.0
23	511.0
24	622.5
25	734.0
26	936.0
27	1138.0
28	1255.0
29	1372.0
30	1634.5
31	1897.0
32	2163.5
33	2430.0
34	3041.5
35	3653.0
36	3801.0
37	3949.0
38	4890.0
39	7071.5
40	8312.0
41	13866.5
42	19421.0
43	26781.5
44	34142.0
45	37176.0
46	40210.0
47	40768.0
48	41326.0
49	45724.0
50	50122.0
51	50488.5
52	50855.0
53	43992.5
54	37130.0
55	30060.5
56	22991.0
57	18200.0
58	13409.0
59	12054.5
60	10700.0
61	9634.5
62	8569.0
63	7809.5
64	5615.0
65	4180.0
66	3375.0
67	2570.0
68	2042.5
69	1515.0
70	1293.0
71	1071.0
72	897.5
73	724.0
74	620.0
75	516.0
76	469.0
77	422.0
78	288.5
79	155.0
80	114.5
81	74.0
82	40.5
83	7.0
84	4.5
85	2.0
86	1.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	383824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.492483668476503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.88386142042522	18.513970573706903
2	12.130475850704041	6.4273286673125085
3	4.3178527373769	3.4317192938353234
4	2.103780839983998	2.229375181813194
5	1.1852872312731144	1.570060130847836
6	0.7965198049138379	1.2661072753978764
7	0.5756528972957536	1.0675332486223255
8	0.4587233991593233	0.9722177728461121
9	0.4417336434088868	1.0532359200443902
>10	7.54844843561112	49.065225679107336
>50	0.4687173727394593	8.334013839610048
>100	0.08195058542566239	3.8165901927750054
>500	0.003997589532959141	0.7527274310134818
>1k	0.0029981921497193556	1.499894793067666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	2373	0.6182521155529618	TruSeq Adapter, Index 16 (100% over 24bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT	1825	0.4754783442411105	TruSeq Adapter, Index 16 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	1467	0.38220642794614196	TruSeq Adapter, Index 16 (100% over 24bp)
TGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTTG	980	0.25532535745550045	TruSeq Adapter, Index 16 (100% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	651	0.16960898745258243	No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	625	0.16283504939764057	TruSeq Adapter, Index 16 (100% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	587	0.15293467839426403	TruSeq Adapter, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	497	0.1294864312810038	TruSeq Adapter, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	493	0.1284442869648589	TruSeq Adapter, Index 16 (100% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	488	0.12714160656967777	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	483	0.12583892617449663	No Hit
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	452	0.11776230772437365	TruSeq Adapter, Index 16 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.48407603484930595	0.0
2	0.0	0.0	0.0	1.774511234315728	0.0
3	0.0	0.0	0.0	2.7661115511276	0.0
4	0.0	0.0	0.0	3.3744632956771854	0.0
5	0.0	0.0	0.0	6.492298553503689	0.0
6	0.0	0.0	0.0	7.121753720455208	0.0
7	0.0	0.0	0.0	10.630132560757014	0.0
8	0.0	0.0	0.0	14.01762266038601	0.0
9	0.0	0.0	0.0	17.675028137896536	0.0
10	0.0	0.0	0.0	18.907624327816915	0.0
11	0.0	0.0	0.0	20.142565342448624	0.0
12	0.0	0.0	0.0	21.261046729751136	0.0
13	0.0	0.0	0.0	21.612249364291966	0.0
14	0.0	0.0	0.0	21.910563174788443	0.0
15	2.6053607903622495E-4	0.0	0.0	22.962347325857685	0.0
16	2.6053607903622495E-4	0.0	0.0	23.966453374463295	0.0
17	2.6053607903622495E-4	0.0	0.0	25.71699528950769	0.0
18	2.6053607903622495E-4	0.0	0.0	26.26646588019509	0.0
19	2.6053607903622495E-4	0.0	0.0	27.099920797031974	0.0
20	2.6053607903622495E-4	0.0	0.0	27.962555754720913	0.0
21	2.6053607903622495E-4	0.0	0.0	28.612853807995332	0.0
22	2.6053607903622495E-4	0.0	0.0	29.491642002584516	0.0
23	2.6053607903622495E-4	0.0	0.0	30.037725624244445	0.0
24	5.210721580724499E-4	0.0	0.0	30.459012464046022	0.0
25	7.816082371086749E-4	0.0	0.0	30.955594230689066	0.0
26	7.816082371086749E-4	0.0	0.0	31.37688107049064	0.0
27	7.816082371086749E-4	0.0	0.0	31.84897244570428	0.0
28	7.816082371086749E-4	0.0	0.0	32.23769227562633	0.0
29	7.816082371086749E-4	0.0	0.0	32.84604402017592	0.0
30	7.816082371086749E-4	0.0	0.0	33.41974446621368	0.0
31	7.816082371086749E-4	0.0	0.0	33.832694151486095	0.0
32	7.816082371086749E-4	0.0	0.0	34.235743465755135	0.0
33	7.816082371086749E-4	0.0	0.0	34.62211847096586	0.0
34	7.816082371086749E-4	0.0	0.0	35.27658510150486	0.0
35	7.816082371086749E-4	0.0	0.0	35.6918796114886	0.0
36	7.816082371086749E-4	0.0	0.0	36.13296719329693	0.0
37	7.816082371086749E-4	0.0	0.0	36.50110467297511	0.0
38	0.0010421443161448998	0.0	0.0	36.826514235691356	0.0
39	0.0010421443161448998	0.0	0.0	37.24545625078161	0.0
40	0.0010421443161448998	0.0	0.0	37.63860519404727	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGCGG	35	1.01719706E-7	46.000004	1
CCACTTA	35	1.01719706E-7	46.000004	25
GTCGGGA	35	1.01719706E-7	46.000004	3
GCCCCGG	20	6.3061755E-4	46.0	1
CGGGTAT	60	0.0	46.0	5
AGACTAC	20	6.3061755E-4	46.0	16
AATCCGG	25	3.4128738E-5	46.0	1
CTCACGG	45	3.092282E-10	46.0	1
GGTACAA	20	6.3061755E-4	46.0	7
CAAACGG	50	1.6370905E-11	46.0	1
ACCGGGT	20	6.3061755E-4	46.0	3
CCGGGAT	20	6.3061755E-4	46.0	4
GTCGAGG	40	5.5952114E-9	46.0	1
CTCCGGT	20	6.3061755E-4	46.0	21
CGAGTCT	25	3.4128738E-5	46.0	16
TTTACGG	30	1.8581413E-6	46.0	1
ATGGACT	25	3.4128738E-5	46.0	39
TGGGTAC	30	1.8581413E-6	46.0	5
TAGCGCC	25	3.4128738E-5	46.0	20
CCGGATA	20	6.3061755E-4	46.0	19
>>END_MODULE