FastQCFastQC Report
Thu 26 May 2016
SRR1544926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544926_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1078254
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT33800.3134697390410794TruSeq Adapter, Index 20 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT31100.28842925692833044No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT28080.26042101397258904TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT26300.2439128442834434TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.18131163900157105No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA18130.168142200260792No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA18070.16758574510273092No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15240.14133961014751625No Hit
ACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC11290.104706312241828TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG750.046.0000041
TTGATCG206.311281E-446.033
ACCGGTA301.8612955E-646.041
CGACGAA253.4170138E-546.036
CGTGCAT206.311281E-446.013
TTATACG253.4170138E-546.044
TCGCTAC206.311281E-446.024
ACGTCGA206.311281E-446.029
CGACTCG206.311281E-446.020
ATTGCGA206.311281E-446.012
CGATAGA206.311281E-446.09
TACCGAA253.4170138E-546.08
CGGGTCA1400.045.9999965
GTACCGG351.019489E-745.9999961
CCGTAGG351.019489E-745.9999961
AGACACG1250.044.1623
CTAGCGG2900.043.620691
ATAGCGG1650.043.212121
AGTACGG1650.043.212121
TTACGGG4700.043.063832
Produced by FastQC (version 0.11.5)