##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544926_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1078254
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92803087213217	34.0	33.0	34.0	31.0	34.0
2	33.25134523034461	34.0	33.0	34.0	31.0	34.0
3	33.31958981835449	34.0	34.0	34.0	31.0	34.0
4	36.5892071812393	37.0	37.0	37.0	35.0	37.0
5	36.58626631572895	37.0	37.0	37.0	35.0	37.0
6	36.66676126404354	37.0	37.0	37.0	35.0	37.0
7	36.50643169420192	37.0	37.0	37.0	35.0	37.0
8	36.53401239411122	37.0	37.0	37.0	35.0	37.0
9	38.312546023478696	39.0	39.0	39.0	37.0	39.0
10	38.1665238431761	39.0	39.0	39.0	35.0	39.0
11	37.59646613877621	39.0	35.0	39.0	35.0	39.0
12	37.546042027203235	39.0	35.0	39.0	35.0	39.0
13	37.53475062462091	39.0	35.0	39.0	35.0	39.0
14	38.88204263559421	41.0	36.0	41.0	35.0	41.0
15	38.92955648669052	41.0	36.0	41.0	35.0	41.0
16	38.92566964741146	41.0	36.0	41.0	35.0	41.0
17	38.95872122894976	41.0	36.0	41.0	35.0	41.0
18	38.947157163339995	41.0	36.0	41.0	35.0	41.0
19	38.884648700584464	41.0	36.0	41.0	35.0	41.0
20	38.81341965807685	41.0	35.0	41.0	35.0	41.0
21	38.762885182897534	41.0	35.0	41.0	35.0	41.0
22	38.7380802668017	41.0	35.0	41.0	35.0	41.0
23	38.71620230483727	41.0	35.0	41.0	35.0	41.0
24	38.65844040458	41.0	35.0	41.0	35.0	41.0
25	38.60986928868337	41.0	35.0	41.0	35.0	41.0
26	38.553249048925395	41.0	35.0	41.0	35.0	41.0
27	38.501860415078454	40.0	36.0	41.0	35.0	41.0
28	38.52773465250303	40.0	36.0	41.0	35.0	41.0
29	38.54686650826243	40.0	36.0	41.0	35.0	41.0
30	38.42674267844126	40.0	35.0	41.0	35.0	41.0
31	38.39287032554481	40.0	35.0	41.0	35.0	41.0
32	38.31275376673771	40.0	35.0	41.0	35.0	41.0
33	38.20182628582876	40.0	35.0	41.0	35.0	41.0
34	38.09950345651396	40.0	35.0	41.0	35.0	41.0
35	38.049358499945285	40.0	35.0	41.0	35.0	41.0
36	37.981388429813386	40.0	35.0	41.0	35.0	41.0
37	37.942791772625	40.0	35.0	41.0	35.0	41.0
38	37.89059349652308	40.0	35.0	41.0	35.0	41.0
39	37.807204053961314	40.0	35.0	41.0	35.0	41.0
40	37.71965325424251	40.0	35.0	41.0	35.0	41.0
41	37.68998306521469	40.0	35.0	41.0	35.0	41.0
42	37.60383824219525	40.0	35.0	41.0	35.0	41.0
43	37.51256939459534	39.0	35.0	41.0	34.0	41.0
44	37.43428635553404	39.0	35.0	41.0	34.0	41.0
45	37.36393187504985	39.0	35.0	41.0	34.0	41.0
46	37.374078834857094	39.0	35.0	41.0	34.0	41.0
47	37.32688494547667	39.0	35.0	41.0	34.0	41.0
48	37.311038957425616	39.0	35.0	41.0	34.0	41.0
49	37.24896174741758	39.0	35.0	41.0	34.0	41.0
50	37.159817631096196	39.0	35.0	41.0	34.0	41.0
51	37.09689924637423	39.0	35.0	41.0	34.0	41.0
52	36.86931743355462	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	7.0
14	13.0
15	16.0
16	26.0
17	54.0
18	91.0
19	162.0
20	258.0
21	495.0
22	696.0
23	1146.0
24	1940.0
25	3133.0
26	4389.0
27	5208.0
28	5672.0
29	6094.0
30	7146.0
31	8665.0
32	11175.0
33	15634.0
34	47779.0
35	192764.0
36	39873.0
37	62925.0
38	111646.0
39	545822.0
40	5420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.55647185171583	22.57779706822326	23.938608157261648	19.927122922799267
2	26.64928671722989	24.398611087925477	26.321627371658256	22.630474823186372
3	27.76692690219559	24.46566393447184	24.777278822986048	22.990130340346525
4	26.35102675250915	27.221971817401098	22.421804138913465	24.00519729117629
5	24.12919404889757	36.781964175416924	19.62979038334196	19.45905139234355
6	87.60672346218979	3.3354849599445027	6.500323671416938	2.5574679064487587
7	87.8447935273136	2.8989458884455797	5.007725452444415	4.248535131796404
8	77.65897460153174	5.607491370307923	9.862889449053748	6.870644579106592
9	47.42722957670456	20.357262759980486	15.910165879282617	16.305341784032336
10	37.81057153509285	22.794814579867083	24.436171811094603	14.958442073945472
11	37.98066132840685	18.211293442917903	24.656064341055075	19.15198088762017
12	21.83956655852888	31.168444540896672	27.79929404388947	19.192694856684973
13	15.861754280531304	32.406928237687964	28.907381748641786	22.823935733138946
14	14.162154742760055	24.751867370767926	40.69968671574601	20.386291170726007
15	17.413614973837333	19.059702073908376	41.86814980514795	21.65853314710634
16	18.304963394524854	22.475316576613675	27.471170985686115	31.748549043175355
17	19.555318134688115	23.78539750374216	31.63466122082552	25.0246231407442
18	23.67113871128695	27.735858155870506	26.561181317203552	22.031821815638985
19	25.868394645417496	24.109996345944463	25.689308827048173	24.332300181589865
20	21.915615430130565	29.765250117319297	26.540128763723576	21.779005688826565
21	21.48232234705366	24.860097899010807	24.895896514179405	28.761683239756124
22	19.75944443517019	28.80935289829669	24.536333739545597	26.89486892698752
23	19.64110497155587	22.245871566439817	35.17992977535906	22.93309368664526
24	20.56500601898996	23.357761714772217	31.3603288279014	24.71690343833642
25	18.153607591532236	29.42201002732195	28.06889656797007	24.355485813175743
26	19.57099162164017	27.85169357127356	28.251321117287766	24.32599368979851
27	20.15833004097365	25.773797268547117	34.08695910240073	19.980913588078504
28	18.475053187838856	25.48972691035693	29.210464324732392	26.824755577071823
29	22.46288907808364	28.05433599133414	26.376716432306303	23.106058498275917
30	24.65114898715887	25.806906350451747	25.58580816764881	23.95613649474057
31	26.868808277084995	25.418222422546076	24.146165931218434	23.566803369150495
32	24.328775965588815	27.13275350705863	24.977973649993416	23.56049687735914
33	19.782444581703384	25.924596616381667	26.73729937472989	27.55565942718506
34	19.32476021419814	24.240670565562475	30.575448827456242	25.859120392783147
35	25.035474016326393	26.01622623240906	23.71806642961677	25.230233321647777
36	19.73700074379506	29.66907611750107	26.387660050414834	24.206263088289028
37	19.987127337343523	28.012230884374183	28.244829140443716	23.75581263783858
38	18.784256770668136	27.966416076360485	25.582283951647756	27.667043201323622
39	24.792674082359074	22.94357359212208	27.01497049860237	25.248781826916478
40	16.780554489016502	21.107086085467802	30.908209011976766	31.204150413538922
41	20.458444856221263	24.781266751618823	25.54323934805714	29.217049044102783
42	21.164957421906156	22.849718155462444	27.583760412667147	28.401564009964257
43	21.878703904645842	27.00143008975622	26.781630302322085	24.338235703275853
44	18.471807199416833	29.22474667378929	26.80435222127625	25.499093905517622
45	21.527951670014673	27.50019939643164	26.166098154980183	24.80575077857351
46	22.290109751505675	23.544545162828054	28.410281807440548	25.755063278225727
47	21.00089589280448	21.502447475270206	30.43243985183454	27.064216780090778
48	19.83252554592888	21.376132154390337	28.765949395967926	30.025392903712856
49	20.215459437201254	25.22930589638434	30.073248047306105	24.481986619108298
50	19.377159741582226	25.352375228842185	27.993033181421072	27.277431848154514
51	19.586386881013194	22.06326153206944	30.433923732256034	27.91642785466133
52	21.353317492909834	20.692526992712292	31.590515778286004	26.363639736091866
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	334.5
2	558.0
3	2201.0
4	3844.0
5	2644.0
6	1444.0
7	1454.0
8	1464.0
9	1489.5
10	1515.0
11	1439.5
12	1364.0
13	1328.0
14	1184.0
15	1076.0
16	1066.0
17	1056.0
18	1040.0
19	1024.0
20	1149.5
21	1275.0
22	1686.5
23	2098.0
24	2821.0
25	3544.0
26	4340.0
27	5136.0
28	8601.0
29	12066.0
30	11754.0
31	11442.0
32	13046.5
33	14651.0
34	17475.0
35	20299.0
36	22050.0
37	23801.0
38	25914.0
39	31488.5
40	34950.0
41	44211.5
42	53473.0
43	66336.0
44	79199.0
45	88932.0
46	98665.0
47	106626.0
48	114587.0
49	114244.0
50	113901.0
51	103112.0
52	92323.0
53	81702.5
54	71082.0
55	61965.5
56	52849.0
57	48311.0
58	43773.0
59	43497.0
60	43221.0
61	42533.0
62	41845.0
63	38107.0
64	27933.5
65	21498.0
66	18361.5
67	15225.0
68	12633.5
69	10042.0
70	8877.0
71	7712.0
72	6301.0
73	4890.0
74	4205.0
75	3520.0
76	2643.0
77	1766.0
78	1479.5
79	1193.0
80	866.5
81	540.0
82	445.5
83	351.0
84	263.5
85	176.0
86	93.0
87	10.0
88	6.0
89	3.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1078254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.16364764080411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84847788233394	16.411091775536867
2	11.748540232953113	5.442780924998731
3	4.246273801807984	2.950775703943734
4	2.0068075393816263	1.859399309005804
5	1.159716380151607	1.3431630796550327
6	0.7322895564275966	1.0177498353677752
7	0.5563071619124138	0.902027215501644
8	0.42269533393390885	0.7832932619725677
9	0.3813458155727091	0.7950024091099172
>10	6.336636561794649	38.96943981516629
>50	1.3797076017991319	20.17571591677609
>100	0.1699523321661719	6.227456507738684
>500	0.007232014134730715	1.1521641445109376
>1k	0.0040177856304059525	1.9699401007159143
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	3380	0.3134697390410794	TruSeq Adapter, Index 20 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	3110	0.28842925692833044	No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT	2808	0.26042101397258904	TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	2630	0.2439128442834434	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1955	0.18131163900157105	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1813	0.168142200260792	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1807	0.16758574510273092	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1524	0.14133961014751625	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	1129	0.104706312241828	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2659855655531999	0.0
2	0.0	0.0	0.0	1.0046797878792937	0.0
3	0.0	0.0	0.0	1.5956351657401688	0.0
4	0.0	0.0	0.0	1.9524156645836694	0.0
5	0.0	0.0	0.0	3.9710494929766087	0.0
6	0.0	0.0	0.0	4.371418979201561	0.0
7	0.0	0.0	0.0	6.225805793440135	0.0
8	0.0	0.0	0.0	8.275601110684496	0.0
9	9.27425263435146E-5	0.0	0.0	10.261404084751831	0.0
10	9.27425263435146E-5	0.0	0.0	11.196712462926175	0.0
11	9.27425263435146E-5	0.0	0.0	12.047254172022548	0.0
12	9.27425263435146E-5	0.0	0.0	12.758867576656336	0.0
13	9.27425263435146E-5	0.0	0.0	13.036631443055162	0.0
14	9.27425263435146E-5	0.0	0.0	13.258286081016161	0.0
15	9.27425263435146E-5	0.0	0.0	14.007460208819072	0.0
16	9.27425263435146E-5	0.0	0.0	14.793545862106702	0.0
17	9.27425263435146E-5	0.0	0.0	15.885589109801586	0.0
18	9.27425263435146E-5	0.0	0.0	16.369705097314732	0.0
19	9.27425263435146E-5	0.0	0.0	16.997015545502265	0.0
20	9.27425263435146E-5	0.0	0.0	17.73385491730149	0.0
21	9.27425263435146E-5	0.0	0.0	18.393161537077535	0.0
22	1.854850526870292E-4	0.0	0.0	19.11423467939836	0.0
23	1.854850526870292E-4	0.0	0.0	19.631645233868827	0.0
24	2.782275790305438E-4	0.0	0.0	20.11270071801264	0.0
25	2.782275790305438E-4	0.0	0.0	20.557493874356133	0.0
26	2.782275790305438E-4	0.0	0.0	20.967601325847156	0.0
27	2.782275790305438E-4	0.0	0.0	21.388466910394026	0.0
28	2.782275790305438E-4	0.0	0.0	21.809610722519924	0.0
29	2.782275790305438E-4	0.0	0.0	22.341303626047296	0.0
30	2.782275790305438E-4	0.0	0.0	22.855282707043052	0.0
31	2.782275790305438E-4	0.0	0.0	23.31250336191658	0.0
32	2.782275790305438E-4	0.0	0.0	23.77426840058094	0.0
33	2.782275790305438E-4	0.0	0.0	24.201811447024543	0.0
34	2.782275790305438E-4	0.0	0.0	24.753814963821142	0.0
35	2.782275790305438E-4	0.0	0.0	25.176164428789505	0.0
36	2.782275790305438E-4	0.0	0.0	25.66074412893437	0.0
37	2.782275790305438E-4	0.0	0.0	26.07585967684794	0.0
38	2.782275790305438E-4	0.0	0.0	26.50729790939797	0.0
39	2.782275790305438E-4	0.0	0.0	26.934099015630824	0.0
40	2.782275790305438E-4	0.0	0.0	27.406714929877374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAGG	75	0.0	46.000004	1
TTGATCG	20	6.311281E-4	46.0	33
ACCGGTA	30	1.8612955E-6	46.0	41
CGACGAA	25	3.4170138E-5	46.0	36
CGTGCAT	20	6.311281E-4	46.0	13
TTATACG	25	3.4170138E-5	46.0	44
TCGCTAC	20	6.311281E-4	46.0	24
ACGTCGA	20	6.311281E-4	46.0	29
CGACTCG	20	6.311281E-4	46.0	20
ATTGCGA	20	6.311281E-4	46.0	12
CGATAGA	20	6.311281E-4	46.0	9
TACCGAA	25	3.4170138E-5	46.0	8
CGGGTCA	140	0.0	45.999996	5
GTACCGG	35	1.019489E-7	45.999996	1
CCGTAGG	35	1.019489E-7	45.999996	1
AGACACG	125	0.0	44.16	23
CTAGCGG	290	0.0	43.62069	1
ATAGCGG	165	0.0	43.21212	1
AGTACGG	165	0.0	43.21212	1
TTACGGG	470	0.0	43.06383	2
>>END_MODULE