FastQCFastQC Report
Thu 26 May 2016
SRR1544925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544925_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences626003
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT25970.4148542419125787TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT20680.3303498545534127TruSeq Adapter, Index 22 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA20510.3276342126155945No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT16920.2702862446346104TruSeq Adapter, Index 20 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT13160.21022263471580804No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.20367314533636421No Hit
TGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTTG12100.19328980851529465TruSeq Adapter, Index 20 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8690.1388172261155298No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8320.1329067113096902No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA7700.12300260541882387No Hit
ACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC6940.11086208852034256TruSeq Adapter, Index 22 (95% over 21bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG6590.10527106100130511No Hit
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT6590.10527106100130511TruSeq Adapter, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGA206.309241E-446.023
CGGGTAC405.6043064E-946.05
AGGTACG253.4153614E-546.010
CTCACGT253.4153614E-546.044
GTACCGG301.8600349E-646.01
GTCGATA253.4153614E-546.027
TCGATAG253.4153614E-546.028
ATTCCCG206.309241E-446.038
CTAACGG453.092282E-1046.01
CCGTAGG453.092282E-1046.01
TCGTCGG206.309241E-446.01
CGAATAC206.309241E-446.039
CGCACGA206.309241E-446.038
GGCGAAA206.309241E-446.07
TGTCGAT253.4153614E-546.026
CCCGACG206.309241E-446.09
CACGACG351.0185795E-745.99999625
CGGTCTA351.0185795E-745.99999630
GCTACGA1350.044.29629510
CTAGCGG1350.044.2962951
Produced by FastQC (version 0.11.5)