##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544925_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	626003
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91710423112988	34.0	33.0	34.0	31.0	34.0
2	33.28363921578651	34.0	34.0	34.0	31.0	34.0
3	33.4125587257569	34.0	34.0	34.0	31.0	34.0
4	36.625183904869466	37.0	37.0	37.0	35.0	37.0
5	36.63432603358131	37.0	37.0	37.0	35.0	37.0
6	36.43174872963868	37.0	37.0	37.0	35.0	37.0
7	36.26464250171325	37.0	37.0	37.0	35.0	37.0
8	36.39604123302924	37.0	37.0	37.0	35.0	37.0
9	38.254754370186724	39.0	39.0	39.0	37.0	39.0
10	38.18075152994474	39.0	39.0	39.0	35.0	39.0
11	37.65815020055815	39.0	37.0	39.0	35.0	39.0
12	37.64887548462228	39.0	37.0	39.0	35.0	39.0
13	37.64907676161296	39.0	37.0	39.0	35.0	39.0
14	38.929075419766356	41.0	37.0	41.0	35.0	41.0
15	38.94967755745579	41.0	37.0	41.0	35.0	41.0
16	38.9691886460608	41.0	37.0	41.0	35.0	41.0
17	39.02507336226823	41.0	37.0	41.0	35.0	41.0
18	39.035613247859835	41.0	37.0	41.0	35.0	41.0
19	38.99825240454119	41.0	37.0	41.0	35.0	41.0
20	38.940723926243166	41.0	36.0	41.0	35.0	41.0
21	38.888189034237854	41.0	35.0	41.0	35.0	41.0
22	38.86037287361243	41.0	35.0	41.0	35.0	41.0
23	38.8225056429442	41.0	35.0	41.0	35.0	41.0
24	38.784972276490684	41.0	35.0	41.0	35.0	41.0
25	38.7249470050463	41.0	35.0	41.0	35.0	41.0
26	38.64931477964163	41.0	35.0	41.0	35.0	41.0
27	38.6949104077776	41.0	36.0	41.0	35.0	41.0
28	38.70932567415811	41.0	36.0	41.0	35.0	41.0
29	38.735625867607666	41.0	36.0	41.0	35.0	41.0
30	38.6349218773712	41.0	36.0	41.0	35.0	41.0
31	38.607522647655045	41.0	35.0	41.0	35.0	41.0
32	38.53574663380208	40.0	35.0	41.0	35.0	41.0
33	38.4538205088474	40.0	35.0	41.0	35.0	41.0
34	38.350459981821174	40.0	35.0	41.0	35.0	41.0
35	38.29076697715506	40.0	35.0	41.0	35.0	41.0
36	38.25245086684888	40.0	35.0	41.0	35.0	41.0
37	38.20109328549544	40.0	35.0	41.0	35.0	41.0
38	38.16357908827913	40.0	35.0	41.0	35.0	41.0
39	38.10733175400118	40.0	35.0	41.0	35.0	41.0
40	38.03182892094766	40.0	35.0	41.0	35.0	41.0
41	38.00751913329489	40.0	35.0	41.0	35.0	41.0
42	37.94086609808579	40.0	35.0	41.0	35.0	41.0
43	37.87206451087295	40.0	35.0	41.0	35.0	41.0
44	37.71913553129937	40.0	35.0	41.0	35.0	41.0
45	37.703292156746855	40.0	35.0	41.0	35.0	41.0
46	37.631953840476804	40.0	35.0	41.0	35.0	41.0
47	37.55175454430729	39.0	35.0	41.0	35.0	41.0
48	37.48332675722001	39.0	35.0	41.0	35.0	41.0
49	37.42162417751992	39.0	35.0	41.0	35.0	41.0
50	37.348325806745336	39.0	35.0	41.0	35.0	41.0
51	37.273730317586335	39.0	35.0	41.0	34.0	41.0
52	36.78102980337155	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	6.0
15	10.0
16	12.0
17	25.0
18	57.0
19	82.0
20	134.0
21	240.0
22	385.0
23	522.0
24	930.0
25	1559.0
26	2302.0
27	2750.0
28	2707.0
29	2861.0
30	3240.0
31	3958.0
32	5339.0
33	7438.0
34	26550.0
35	108613.0
36	21904.0
37	35870.0
38	66871.0
39	329625.0
40	2007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.633389935830984	22.10484614290986	23.6489282000246	21.612835721234564
2	26.857379277735088	24.66154315554398	26.231503682889702	22.24957388383123
3	27.63628928295871	24.374803315639063	25.02112609683979	22.967781304562436
4	26.172398534831302	26.77031899208151	22.987110285413966	24.070172187673222
5	24.22081044340043	35.99008311461766	19.84319563963751	19.945910802344397
6	86.51092726392685	3.219153901818362	7.146770862120469	3.123147972134319
7	86.70150143050432	2.689284236657013	5.695180374534947	4.9140339583037145
8	76.06560990921768	5.547577248032358	10.87343031902403	7.513382523725924
9	47.41063541229036	19.39096138516908	16.344490361867276	16.85391284067329
10	37.0110047395939	22.325452114446737	25.526874471847577	15.136668674111785
11	37.010046277733494	17.827390603559408	25.08789893978144	20.074664178925662
12	21.438874893570798	30.130846018309814	28.69235450948318	19.737924578636203
13	15.814620696705925	31.4720536483052	29.358645246109045	23.35468040887983
14	14.189708356030243	24.158989653404216	40.549805671857804	21.10149631870774
15	17.20518911251224	18.65773806195817	41.986060769676826	22.151012055852767
16	18.10406659393006	22.62017913652171	27.831655758838213	31.44409851071001
17	19.19399747285556	23.829917747998014	31.913585078665758	25.062499700480668
18	23.291421926093005	27.877502184494325	26.608019450386024	22.22305643902665
19	25.369207495810723	23.981993696515833	25.795563280048178	24.853235527625266
20	21.53296389953403	30.157842694044596	26.570319950543368	21.738873455878007
21	21.147821975294047	24.637901096320626	24.990295573663385	29.22398135472194
22	19.43728704175539	29.040276164810713	24.656750846241952	26.86568594719195
23	19.47482679795464	22.174174884145923	35.0694804976973	23.28151782020214
24	20.77801544082057	23.535190725923037	30.82876599632909	24.8580278369273
25	18.331701285776585	28.97014870535764	28.296030530205122	24.402119478660644
26	19.816837938476333	26.917602631297292	28.108012261922067	25.157547168304305
27	20.772743900588335	25.339175690851324	33.8012757127362	20.086804695824142
28	18.981218939845338	25.30259439651248	28.860085335054304	26.856101328587883
29	23.100049041298522	27.308655070343114	26.154507246770383	23.43678864158798
30	24.54317311578379	25.589014749130595	25.271124898762466	24.59668723632315
31	27.071595503535928	24.918570677776305	23.49477558414257	24.5150582345452
32	23.99093934054629	26.786932330995217	25.24924001961652	23.97288830884197
33	19.751023557395094	25.49157112665594	26.81185233936579	27.945552976583176
34	19.47115269415642	23.768895676218804	30.935634493764407	25.82431713586037
35	24.573684151673394	25.850994324308353	23.751164131801286	25.824157392216968
36	19.93009618164769	29.443628864398413	25.973038467866765	24.653236486087128
37	20.096229570784804	27.83197524612502	28.146829967268527	23.92496521582165
38	18.79367990249248	27.302584811893873	25.124001003190084	28.77973428242357
39	24.9628196669984	22.374333669327463	26.73437667231627	25.928469991357865
40	16.70918509975192	20.590476403467715	30.741545967032106	31.95879252974826
41	20.594629738196144	24.615696729887876	25.442849315418613	29.346824216497364
42	20.96619345274703	22.45708087660922	27.635969795671905	28.940755874971845
43	22.449572925369367	26.596997138991345	26.571118668760374	24.38231126687891
44	18.70438320583128	28.212324861062964	27.432935624909145	25.650356308196603
45	21.527692359301792	26.698434352551025	26.234698555757717	25.539174732389462
46	22.568581939703165	22.604204772181603	28.381333635781296	26.445879652333936
47	20.959324476080784	20.799101601749513	30.595220789676723	27.646353132492973
48	19.778339720416678	20.43137173464025	28.67126834855424	31.119020196388835
49	20.03408929350179	24.41330153369872	30.318544799306075	25.23406437349342
50	19.474028079737636	24.59397159438533	28.248107437184807	27.683892888692224
51	19.595912479652654	21.36587204853651	30.49713819262847	28.54107727918237
52	21.42625514574211	20.249743212093232	31.835310693399233	26.48869094876542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	217.5
2	377.0
3	1435.5
4	2494.0
5	1715.5
6	937.0
7	930.0
8	923.0
9	917.0
10	911.0
11	861.5
12	812.0
13	812.0
14	736.5
15	661.0
16	636.5
17	612.0
18	637.5
19	663.0
20	723.0
21	783.0
22	946.5
23	1110.0
24	1423.5
25	1737.0
26	2195.5
27	2654.0
28	4248.5
29	5843.0
30	5868.0
31	5893.0
32	6733.5
33	7574.0
34	9001.0
35	10428.0
36	11349.5
37	12271.0
38	13694.5
39	16842.0
40	18566.0
41	23762.0
42	28958.0
43	36441.5
44	43925.0
45	50199.0
46	56473.0
47	62706.0
48	68939.0
49	69347.5
50	69756.0
51	63246.0
52	56736.0
53	49703.0
54	42670.0
55	37245.5
56	31821.0
57	28576.0
58	25331.0
59	25105.5
60	24880.0
61	24692.0
62	24504.0
63	22867.5
64	16945.0
65	12659.0
66	10842.0
67	9025.0
68	7551.0
69	6077.0
70	5274.0
71	4471.0
72	3700.5
73	2930.0
74	2506.5
75	2083.0
76	1569.5
77	1056.0
78	842.0
79	628.0
80	472.0
81	316.0
82	256.5
83	197.0
84	144.0
85	91.0
86	48.5
87	6.0
88	3.0
89	1.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	626003.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.037388708108363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.80854140331672	20.133239878870842
2	10.47992952879671	5.876597156649125
3	3.4933138052315744	2.938301911100364
4	1.7249369880720593	1.9345091532628003
5	1.111332112040669	1.5579425204543633
6	0.8009050836047891	1.3473172288396498
7	0.6771474972023862	1.3289813314250214
8	0.6036446979465045	1.3539696830331849
9	0.5837716723797077	1.4730689965763117
>10	8.36187814091293	50.46999106465486
>50	0.2761175314686062	5.134360842419769
>100	0.06874295389259888	3.4262265927518465
>500	0.005728579491049906	1.0645523090549052
>1k	0.004010005643734934	1.960941330906961
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	2597	0.4148542419125787	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT	2068	0.3303498545534127	TruSeq Adapter, Index 22 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2051	0.3276342126155945	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	1692	0.2702862446346104	TruSeq Adapter, Index 20 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1316	0.21022263471580804	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1275	0.20367314533636421	No Hit
TGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTTG	1210	0.19328980851529465	TruSeq Adapter, Index 20 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	869	0.1388172261155298	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	832	0.1329067113096902	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	770	0.12300260541882387	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	694	0.11086208852034256	TruSeq Adapter, Index 22 (95% over 21bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	659	0.10527106100130511	No Hit
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	659	0.10527106100130511	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33578113842904905	0.0
2	0.0	0.0	0.0	1.2138919462047306	0.0
3	0.0	0.0	0.0	1.8870516594968394	0.0
4	0.0	0.0	0.0	2.3332156555160277	0.0
5	0.0	0.0	0.0	4.666750798318858	0.0
6	0.0	0.0	0.0	5.151253268754303	0.0
7	0.0	0.0	0.0	7.367696320944149	0.0
8	0.0	0.0	0.0	9.634618364448732	0.0
9	0.0	0.0	0.0	11.943393242524397	0.0
10	0.0	0.0	0.0	12.994666159746838	0.0
11	0.0	0.0	0.0	13.955044943874071	0.0
12	0.0	0.0	0.0	14.794977020876896	0.0
13	1.5974364340106998E-4	0.0	0.0	15.10887328016	0.0
14	3.1948728680213995E-4	0.0	0.0	15.370054137120748	0.0
15	3.1948728680213995E-4	0.0	0.0	16.211423906914185	0.0
16	3.1948728680213995E-4	0.0	0.0	17.07531753042717	0.0
17	3.1948728680213995E-4	0.0	0.0	18.195759445242274	0.0
18	3.1948728680213995E-4	0.0	0.0	18.709175515133314	0.0
19	3.1948728680213995E-4	0.0	0.0	19.358373681915264	0.0
20	3.1948728680213995E-4	0.0	0.0	20.111884447838108	0.0
21	3.1948728680213995E-4	0.0	0.0	20.796705447098496	0.0
22	3.1948728680213995E-4	0.0	0.0	21.55133441852515	0.0
23	3.1948728680213995E-4	0.0	0.0	22.092705626011377	0.0
24	3.1948728680213995E-4	0.0	0.0	22.595898102724746	0.0
25	3.1948728680213995E-4	0.0	0.0	23.06506518339369	0.0
26	3.1948728680213995E-4	0.0	0.0	23.507555075614654	0.0
27	3.1948728680213995E-4	0.0	0.0	23.922888548457436	0.0
28	3.1948728680213995E-4	0.0	0.0	24.352758692849715	0.0
29	3.1948728680213995E-4	0.0	0.0	24.87480091948441	0.0
30	3.1948728680213995E-4	0.0	0.0	25.348920053098787	0.0
31	3.1948728680213995E-4	0.0	0.0	25.808662258807065	0.0
32	3.1948728680213995E-4	0.0	0.0	26.24939497095062	0.0
33	3.1948728680213995E-4	0.0	0.0	26.683418450071326	0.0
34	3.1948728680213995E-4	0.0	0.0	27.238847098176844	0.0
35	3.1948728680213995E-4	0.0	0.0	27.6497077490044	0.0
36	3.1948728680213995E-4	0.0	0.0	28.11583970044872	0.0
37	3.1948728680213995E-4	0.0	0.0	28.516636501742003	0.0
38	3.1948728680213995E-4	0.0	0.0	28.955292546521342	0.0
39	3.1948728680213995E-4	0.0	0.0	29.368070121069707	0.0
40	3.1948728680213995E-4	0.0	0.0	29.842189254684083	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGA	20	6.309241E-4	46.0	23
CGGGTAC	40	5.6043064E-9	46.0	5
AGGTACG	25	3.4153614E-5	46.0	10
CTCACGT	25	3.4153614E-5	46.0	44
GTACCGG	30	1.8600349E-6	46.0	1
GTCGATA	25	3.4153614E-5	46.0	27
TCGATAG	25	3.4153614E-5	46.0	28
ATTCCCG	20	6.309241E-4	46.0	38
CTAACGG	45	3.092282E-10	46.0	1
CCGTAGG	45	3.092282E-10	46.0	1
TCGTCGG	20	6.309241E-4	46.0	1
CGAATAC	20	6.309241E-4	46.0	39
CGCACGA	20	6.309241E-4	46.0	38
GGCGAAA	20	6.309241E-4	46.0	7
TGTCGAT	25	3.4153614E-5	46.0	26
CCCGACG	20	6.309241E-4	46.0	9
CACGACG	35	1.0185795E-7	45.999996	25
CGGTCTA	35	1.0185795E-7	45.999996	30
GCTACGA	135	0.0	44.296295	10
CTAGCGG	135	0.0	44.296295	1
>>END_MODULE