FastQCFastQC Report
Thu 26 May 2016
SRR1544924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544924_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences373824
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT13150.3517698168121897TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT13140.35150231124807396TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT10880.2910460537579182TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.18484634480397194No Hit
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC5590.1495356103406951TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT5110.13669534326313987TruSeq Adapter, Index 13 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4510.12064500941619585No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA3920.10486218113336758No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT3740.10004708097928437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATG206.3059625E-446.00000418
AATCCTC206.3059625E-446.00000413
GTCGCAA206.3059625E-446.0000044
GTACCCA206.3059625E-446.00000419
AATTTCT206.3059625E-446.00000430
GTACAAC206.3059625E-446.00000446
TTTACGG405.5933924E-946.0000041
CGCGGGT405.5933924E-946.0000043
CAACGCA405.5933924E-946.00000416
CAACGAA206.3059625E-446.00000419
ACGTTGG206.3059625E-446.00000433
TTAGCGG800.046.0000041
CCCTAGG206.3059625E-446.0000041
AACCCGG206.3059625E-446.0000041
GCAACGA206.3059625E-446.00000418
GACGTTC206.3059625E-446.00000438
CGTTGGT206.3059625E-446.00000434
GGCAACG206.3059625E-446.0000047
CCGGATA206.3059625E-446.00000419
GCCGGAT206.3059625E-446.00000418
Produced by FastQC (version 0.11.5)