##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544924_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	373824
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.947820364663585	34.0	33.0	34.0	31.0	34.0
2	33.234334874165384	34.0	33.0	34.0	31.0	34.0
3	33.278585644581405	34.0	34.0	34.0	31.0	34.0
4	36.55968049135422	37.0	37.0	37.0	35.0	37.0
5	36.548552259887	37.0	37.0	37.0	35.0	37.0
6	36.67163692004794	37.0	37.0	37.0	35.0	37.0
7	36.50824719654169	37.0	37.0	37.0	35.0	37.0
8	36.54799852336929	37.0	37.0	37.0	35.0	37.0
9	38.339301382468754	39.0	39.0	39.0	37.0	39.0
10	38.09010924927239	39.0	39.0	39.0	35.0	39.0
11	37.06047765793529	39.0	35.0	39.0	35.0	39.0
12	36.90220531587057	39.0	35.0	39.0	35.0	39.0
13	36.883191020373225	39.0	35.0	39.0	35.0	39.0
14	38.057361218113336	40.0	35.0	41.0	35.0	41.0
15	38.14300044940935	40.0	35.0	41.0	35.0	41.0
16	38.178455636877246	40.0	35.0	41.0	35.0	41.0
17	38.31038670604349	40.0	36.0	41.0	35.0	41.0
18	38.29078924841637	40.0	36.0	41.0	35.0	41.0
19	38.16307139188495	40.0	35.0	41.0	35.0	41.0
20	38.050826057181986	40.0	35.0	41.0	35.0	41.0
21	37.99722061718884	40.0	35.0	41.0	35.0	41.0
22	37.98692165297038	40.0	35.0	41.0	35.0	41.0
23	37.969485640301315	40.0	35.0	41.0	35.0	41.0
24	37.92229230868002	40.0	35.0	41.0	35.0	41.0
25	37.8923343605547	40.0	35.0	41.0	35.0	41.0
26	37.84092514124294	40.0	35.0	41.0	35.0	41.0
27	37.88368858072248	40.0	35.0	41.0	35.0	41.0
28	37.90966337099812	40.0	35.0	41.0	35.0	41.0
29	37.93021047337785	40.0	35.0	41.0	35.0	41.0
30	37.809549413627806	40.0	35.0	41.0	35.0	41.0
31	37.713188559322035	40.0	35.0	41.0	35.0	41.0
32	37.660190356959426	39.0	35.0	41.0	35.0	41.0
33	37.596676510871426	39.0	35.0	41.0	35.0	41.0
34	37.508873159561716	39.0	35.0	41.0	35.0	41.0
35	37.438224940078754	39.0	35.0	41.0	35.0	41.0
36	37.3714903269988	39.0	35.0	41.0	35.0	41.0
37	37.33837581321691	39.0	35.0	41.0	35.0	41.0
38	37.32769699109741	39.0	35.0	41.0	35.0	41.0
39	37.23869253980483	39.0	35.0	41.0	34.0	41.0
40	37.20373223763054	38.0	35.0	41.0	34.0	41.0
41	37.21604284369115	38.0	35.0	41.0	34.0	41.0
42	37.096355504194484	38.0	35.0	41.0	34.0	41.0
43	37.00390023112481	38.0	35.0	41.0	34.0	41.0
44	36.94392548365006	38.0	35.0	41.0	34.0	41.0
45	36.92716090994693	38.0	35.0	41.0	34.0	41.0
46	36.9496153269988	38.0	35.0	41.0	34.0	41.0
47	36.94113540061633	38.0	35.0	41.0	34.0	41.0
48	36.99794823232323	37.0	35.0	41.0	35.0	41.0
49	36.90326998801575	37.0	35.0	41.0	34.0	41.0
50	36.84045700650574	37.0	35.0	41.0	34.0	41.0
51	36.754606445814076	37.0	35.0	41.0	34.0	41.0
52	36.58472168721109	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	8.0
15	9.0
16	10.0
17	8.0
18	31.0
19	53.0
20	101.0
21	182.0
22	277.0
23	464.0
24	755.0
25	1205.0
26	1658.0
27	1942.0
28	1829.0
29	2056.0
30	2393.0
31	3062.0
32	4233.0
33	6106.0
34	19994.0
35	110098.0
36	10853.0
37	14930.0
38	28104.0
39	161930.0
40	1527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.373116760828626	23.00119842492724	25.75730825201164	20.868376562232495
2	25.2431625577812	24.116696627289848	28.43503894881014	22.205101866118817
3	25.734302773497692	25.889456000684813	26.536551960280775	21.839689265536723
4	25.54517633966786	28.159508217770927	23.740048793014896	22.55526664954631
5	22.879215887690464	36.37701164184215	20.783042287279578	19.96073018318781
6	87.85685242253038	2.753434771443246	6.737662643382983	2.6520501626433832
7	87.49197483307653	2.0627354048964217	5.050237544940934	5.395052217086115
8	78.38153783598699	3.63914569423044	11.24406137647663	6.73525509330594
9	55.0325821777093	16.501348228043145	13.798472008217772	14.66759758602979
10	49.236539120013695	17.356296010957028	24.214603663756208	9.19256120527307
11	48.2470360383496	16.067989214175654	20.58589068652628	15.099084060948467
12	19.24702533812703	41.338437339496664	24.84778933401815	14.56674798835816
13	10.970938195514467	45.074687553501114	25.345082177709294	18.609292073275125
14	9.363497260743022	22.659593819551446	52.51187724704673	15.46503167265879
15	9.758335473377846	16.024118301660675	55.06762540660846	19.14992081835302
16	10.006848142441363	19.34519988015751	29.703817839411062	40.94413413799007
17	16.127107943845232	22.538146293442903	38.25409818524225	23.080647577469612
18	24.166452662215374	28.491482622838554	26.406811761684644	20.935252953261426
19	27.99258474576271	22.409208611539118	25.26884309193631	24.329363550761858
20	16.18863422359185	35.23636791645266	26.414301917479882	22.160695942475602
21	17.954438452319806	25.816694487245336	23.559482537236775	32.66938452319808
22	14.883474576271185	35.82407764081493	20.689682845403183	28.6027649375107
23	14.53143725389488	23.132008645779834	41.49492809450436	20.84162600582092
24	14.163617103235747	24.017987074131142	38.559054528334194	23.25934129429892
25	12.701699195343263	34.521593049135426	30.52024482109228	22.256462934429035
26	16.239192775209723	32.337945129258685	26.951453090224277	24.471409005307308
27	16.538531501455232	28.08968926553672	39.62051660674542	15.751262626262626
28	13.430919363122754	25.283555897962678	35.20694230439993	26.078582434514637
29	16.528901301147066	30.965641585344976	30.119253980482796	22.386203133025166
30	28.064008731381612	24.106263910289332	21.457691319979453	26.3720360383496
31	29.640954031843865	26.66976973121041	23.832338212634824	19.856938024310907
32	23.036509159390516	26.431422273583287	26.046214261256633	24.48585430576956
33	16.335227272727273	27.354583975346685	27.2834274952919	29.02676125663414
34	14.7221687211094	24.07015065913371	31.654200907378872	29.553479712378017
35	29.761064030131827	23.83822333504537	25.55320150659134	20.847511128231467
36	19.820557267591166	27.151814757746962	28.716989813388118	24.310638161273754
37	20.336040489642183	31.070771272042457	24.51073232323232	24.082455915083035
38	15.96419705529875	30.559300633453173	24.536947868515664	28.93955444273241
39	24.436633281972263	23.627161444958055	25.716647406266052	26.219557866803626
40	12.942989214175654	18.92307609998288	30.401739856189007	37.73219482965246
41	20.34620570107858	23.94469054956343	24.76058252011642	30.948521229241564
42	20.526771956856702	23.17721708611539	26.656929464132855	29.639081492895052
43	22.056101266906353	29.071434685841464	28.213544341722308	20.658919705529875
44	14.373073959938369	34.1642056154768	26.255671117959256	25.207049306625578
45	21.26321477486732	31.5798343605547	25.423193802431094	21.733757062146893
46	22.20536937168293	22.897138760486218	27.6472350624893	27.25025680534155
47	22.420978856360215	19.41073874336586	31.278623095360388	26.889659304913543
48	19.35509758602979	17.575650573531927	29.952330508474578	33.1169213319637
49	19.214924670433145	27.225111282314675	28.838704845060775	24.721259202191405
50	18.74384737202534	29.626508731381612	24.8946028077384	26.73504108885465
51	17.577255606916626	21.712891628145865	32.540714346858415	28.169138418079097
52	22.731552816298578	19.908566598185242	32.26732366033214	25.092556925184045
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	99.0
2	120.0
3	590.0
4	1060.0
5	750.5
6	441.0
7	543.0
8	645.0
9	684.0
10	723.0
11	754.0
12	785.0
13	752.5
14	675.5
15	631.0
16	624.5
17	618.0
18	546.0
19	474.0
20	580.0
21	686.0
22	604.5
23	523.0
24	665.5
25	808.0
26	910.0
27	1012.0
28	1659.5
29	2307.0
30	2688.5
31	3070.0
32	3372.5
33	3675.0
34	4393.5
35	5112.0
36	5630.5
37	6149.0
38	6608.5
39	9293.5
40	11519.0
41	17011.5
42	22504.0
43	31635.5
44	40767.0
45	45152.0
46	49537.0
47	51575.5
48	53614.0
49	50426.0
50	47238.0
51	41266.5
52	35295.0
53	29387.5
54	23480.0
55	18631.5
56	13783.0
57	11930.5
58	10078.0
59	9281.0
60	8484.0
61	8139.5
62	7795.0
63	6637.0
64	4308.5
65	3138.0
66	2472.5
67	1807.0
68	1414.5
69	1022.0
70	744.0
71	466.0
72	542.0
73	618.0
74	461.5
75	305.0
76	205.5
77	106.0
78	77.0
79	48.0
80	35.0
81	22.0
82	16.5
83	11.0
84	7.0
85	3.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	373824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.967364321177882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11948486082242	18.006334531758263
2	10.89313647759659	5.439458140729327
3	3.755330319069149	2.812821006676939
4	1.708916364883108	1.7066854990583806
5	1.0232069770930208	1.277339068652628
6	0.6964235969743073	1.0432717000513612
7	0.5174963035978314	0.9044363122752953
8	0.4499967857372447	0.8988186954288648
9	0.37392590052928193	0.8402349768875192
>10	7.667088092227912	50.378787878787875
>50	0.6749951786058671	10.9375
>100	0.1135706173527332	4.288916709467557
>500	0.003214262755266034	0.47107729840780693
>1k	0.003214262755266034	0.9943181818181818
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT	1315	0.3517698168121897	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1314	0.35150231124807396	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1088	0.2910460537579182	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	691	0.18484634480397194	No Hit
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	559	0.1495356103406951	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	511	0.13669534326313987	TruSeq Adapter, Index 13 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	451	0.12064500941619585	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	392	0.10486218113336758	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	374	0.10004708097928437	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35551489470980996	0.0
2	0.0	0.0	0.0	1.2425633453175826	0.0
3	0.0	0.0	0.0	2.0592578325629174	0.0
4	0.0	0.0	0.0	2.53060263653484	0.0
5	0.0	0.0	0.0	5.150284625920219	0.0
6	0.0	0.0	0.0	5.750032100667694	0.0
7	0.0	0.0	0.0	9.037407978085945	0.0
8	0.0	0.0	0.0	12.368119756890943	0.0
9	0.0	0.0	0.0	16.1452983222051	0.0
10	0.0	0.0	0.0	17.255446413285398	0.0
11	0.0	0.0	0.0	18.326271186440678	0.0
12	0.0	0.0	0.0	19.210109570279062	0.0
13	0.0	0.0	0.0	19.49794555726759	0.0
14	0.0	0.0	0.0	19.722115219996574	0.0
15	0.0	0.0	0.0	20.544427324088343	0.0
16	2.675055641157336E-4	0.0	0.0	21.383592278719398	0.0
17	2.675055641157336E-4	0.0	0.0	22.810199452148606	0.0
18	2.675055641157336E-4	0.0	0.0	23.252653655196028	0.0
19	2.675055641157336E-4	0.0	0.0	23.93666538263996	0.0
20	2.675055641157336E-4	0.0	0.0	24.63833247731553	0.0
21	2.675055641157336E-4	0.0	0.0	25.200361667522685	0.0
22	2.675055641157336E-4	0.0	0.0	25.902831278890602	0.0
23	2.675055641157336E-4	0.0	0.0	26.34876305427153	0.0
24	2.675055641157336E-4	0.0	0.0	26.705882982366035	0.0
25	2.675055641157336E-4	0.0	0.0	27.10687382297552	0.0
26	2.675055641157336E-4	0.0	0.0	27.43082306111967	0.0
27	2.675055641157336E-4	0.0	0.0	27.771357644239	0.0
28	2.675055641157336E-4	0.0	0.0	28.100656993665467	0.0
29	2.675055641157336E-4	0.0	0.0	28.62042030474234	0.0
30	2.675055641157336E-4	0.0	0.0	29.10246533127889	0.0
31	2.675055641157336E-4	0.0	0.0	29.44620998116761	0.0
32	2.675055641157336E-4	0.0	0.0	29.753038863208356	0.0
33	2.675055641157336E-4	0.0	0.0	30.069765451121384	0.0
34	2.675055641157336E-4	0.0	0.0	30.611999229583976	0.0
35	2.675055641157336E-4	0.0	0.0	30.988914569423045	0.0
36	2.675055641157336E-4	0.0	0.0	31.362619842492723	0.0
37	5.350111282314672E-4	0.0	0.0	31.684696541688066	0.0
38	5.350111282314672E-4	0.0	0.0	31.976545112138332	0.0
39	8.025166923472008E-4	0.0	0.0	32.32911744564287	0.0
40	8.025166923472008E-4	0.0	0.0	32.72128060263653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATATG	20	6.3059625E-4	46.000004	18
AATCCTC	20	6.3059625E-4	46.000004	13
GTCGCAA	20	6.3059625E-4	46.000004	4
GTACCCA	20	6.3059625E-4	46.000004	19
AATTTCT	20	6.3059625E-4	46.000004	30
GTACAAC	20	6.3059625E-4	46.000004	46
TTTACGG	40	5.5933924E-9	46.000004	1
CGCGGGT	40	5.5933924E-9	46.000004	3
CAACGCA	40	5.5933924E-9	46.000004	16
CAACGAA	20	6.3059625E-4	46.000004	19
ACGTTGG	20	6.3059625E-4	46.000004	33
TTAGCGG	80	0.0	46.000004	1
CCCTAGG	20	6.3059625E-4	46.000004	1
AACCCGG	20	6.3059625E-4	46.000004	1
GCAACGA	20	6.3059625E-4	46.000004	18
GACGTTC	20	6.3059625E-4	46.000004	38
CGTTGGT	20	6.3059625E-4	46.000004	34
GGCAACG	20	6.3059625E-4	46.000004	7
CCGGATA	20	6.3059625E-4	46.000004	19
GCCGGAT	20	6.3059625E-4	46.000004	18
>>END_MODULE