FastQCFastQC Report
Thu 26 May 2016
SRR1544923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544923_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences214852
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT11030.5133766499730047TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT9880.4598514326140785TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT6620.3081190773183401TruSeq Adapter, Index 13 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTTG5470.2545938599594139TruSeq Adapter, Index 13 (95% over 24bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4700.2187552361625677No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.195483402528252No Hit
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC3250.15126691862305217TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT3090.1438199318600711TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC2350.10937761808128385TruSeq Adapter, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC2320.10798130806322491TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC2260.10518868802710704TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGG351.0143776E-746.0000042
CTACGAA351.0143776E-746.00000411
TACGAAT351.0143776E-746.00000412
CGGGCAC351.0143776E-746.0000045
CGGGATG351.0143776E-746.0000045
CGAATAT351.0143776E-746.00000414
GCTACGA351.0143776E-746.00000410
AGCTACG351.0143776E-746.0000049
CTAGCGG700.046.0000041
AGTCCGG206.299947E-446.01
ACAAAGG301.8542942E-646.01
ACCGGGT206.299947E-446.03
ATGATGG253.4078228E-546.01
AGTCAGG253.4078228E-546.01
TTTACTC206.299947E-446.037
TACTTAA253.4078228E-546.033
CTCCGGG301.8542942E-646.02
GGATGTT253.4078228E-546.07
TTTACGG301.8542942E-646.01
CACGGGA453.074092E-1046.03
Produced by FastQC (version 0.11.5)