##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544923_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	214852
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95220430808184	34.0	33.0	34.0	31.0	34.0
2	33.275971366335895	34.0	33.0	34.0	31.0	34.0
3	33.38814625882002	34.0	34.0	34.0	31.0	34.0
4	36.62255878465176	37.0	37.0	37.0	35.0	37.0
5	36.60849328840318	37.0	37.0	37.0	35.0	37.0
6	36.44404054884293	37.0	37.0	37.0	35.0	37.0
7	36.27687896784764	37.0	37.0	37.0	35.0	37.0
8	36.4145644443617	37.0	37.0	37.0	35.0	37.0
9	38.2860899596001	39.0	39.0	39.0	37.0	39.0
10	38.099677917822504	39.0	39.0	39.0	35.0	39.0
11	37.151341388490685	39.0	35.0	39.0	35.0	39.0
12	37.0685262413196	39.0	35.0	39.0	35.0	39.0
13	37.0655288291475	39.0	35.0	39.0	35.0	39.0
14	38.200673021428706	40.0	35.0	41.0	35.0	41.0
15	38.26087260067395	40.0	35.0	41.0	35.0	41.0
16	38.31723232736954	40.0	35.0	41.0	35.0	41.0
17	38.45655148660473	40.0	36.0	41.0	35.0	41.0
18	38.44290488336157	40.0	36.0	41.0	35.0	41.0
19	38.33973619049392	40.0	35.0	41.0	35.0	41.0
20	38.230381844246274	40.0	35.0	41.0	35.0	41.0
21	38.17189507195651	40.0	35.0	41.0	35.0	41.0
22	38.16108763241673	40.0	35.0	41.0	35.0	41.0
23	38.1313508833988	40.0	35.0	41.0	35.0	41.0
24	38.101735147915775	40.0	35.0	41.0	35.0	41.0
25	38.069764302868954	40.0	35.0	41.0	35.0	41.0
26	38.01111928211048	40.0	35.0	41.0	35.0	41.0
27	38.141855789101335	40.0	35.0	41.0	35.0	41.0
28	38.17093161804405	40.0	35.0	41.0	35.0	41.0
29	38.189735259620576	40.0	35.0	41.0	35.0	41.0
30	38.09623368644462	40.0	35.0	41.0	35.0	41.0
31	37.99435425316032	40.0	35.0	41.0	35.0	41.0
32	37.95966991231173	40.0	35.0	41.0	35.0	41.0
33	37.91223726099827	40.0	35.0	41.0	35.0	41.0
34	37.820192504607824	40.0	35.0	41.0	35.0	41.0
35	37.75561782063932	40.0	35.0	41.0	35.0	41.0
36	37.711917971440805	40.0	35.0	41.0	35.0	41.0
37	37.6450905739765	40.0	35.0	41.0	35.0	41.0
38	37.65123899242269	40.0	35.0	41.0	35.0	41.0
39	37.58721352372796	40.0	35.0	41.0	35.0	41.0
40	37.571630703926424	40.0	35.0	41.0	35.0	41.0
41	37.56160519799676	40.0	35.0	41.0	35.0	41.0
42	37.48218308416957	39.0	35.0	41.0	35.0	41.0
43	37.41176716995886	39.0	35.0	41.0	35.0	41.0
44	37.290250963453914	39.0	35.0	41.0	35.0	41.0
45	37.30749073781021	39.0	35.0	41.0	35.0	41.0
46	37.25195017965856	39.0	35.0	41.0	35.0	41.0
47	37.21482229627837	38.0	35.0	41.0	35.0	41.0
48	37.22158974549923	38.0	35.0	41.0	35.0	41.0
49	37.14015694524603	38.0	35.0	41.0	35.0	41.0
50	37.070676558747415	38.0	35.0	41.0	35.0	41.0
51	37.01420047288366	38.0	35.0	41.0	34.0	41.0
52	36.61876082140264	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	3.0
16	2.0
17	11.0
18	7.0
19	28.0
20	54.0
21	79.0
22	127.0
23	213.0
24	385.0
25	669.0
26	931.0
27	1004.0
28	935.0
29	953.0
30	1062.0
31	1299.0
32	1847.0
33	2914.0
34	10433.0
35	60961.0
36	5724.0
37	8391.0
38	16082.0
39	99666.0
40	1070.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.637611006646438	22.53504738145328	25.19175990914676	22.635581702753523
2	24.952990896058683	24.495466646808033	28.376277623666525	22.17526483346676
3	25.63764824158025	25.824753784000148	26.625770297693297	21.911827676726308
4	25.37514195818517	28.04069778265969	23.996983970360994	22.587176288794147
5	23.126151955764897	35.842812726900384	20.974903654608752	20.05613166272597
6	86.57820266974475	2.7242008452329975	7.455364623089382	3.242231861932866
7	85.98290916537896	1.9157373447768695	5.818889281924301	6.282464207919871
8	76.15474838493475	3.682069517621432	12.669186230521476	7.493995866922347
9	53.878018356822366	15.956565449704913	14.7599277642284	15.405488429244318
10	47.7826596913224	17.014503006720904	25.807067190438072	9.395770111518626
11	46.84759741589559	15.73827565021503	21.405432576843594	16.00869435704578
12	18.65935620799434	39.665444119673076	26.392121088004767	15.283078584327816
13	11.02060953586655	43.33820490384078	26.1119282110476	19.52925734924506
14	9.180738368737549	22.110103699290676	52.3160128832871	16.393145048684676
15	9.62383408113492	15.933293616070598	54.64691974010017	19.795952562694318
16	9.708078118891143	19.888574460558896	30.559641055238025	39.84370636531194
17	15.614004058607787	22.589968908830265	38.71362612402957	23.082400908532385
18	23.59438124848733	29.034405078844973	26.643456891255372	20.72775678141232
19	27.267607469327725	22.730530783981532	25.12473702827993	24.877124718410812
20	15.842998901569452	36.03643438273789	25.791242343566733	22.329324372125928
21	17.56697633719956	26.084932884031797	23.484072757060677	32.86401802170797
22	14.722227393740809	36.886787183735784	20.502485431832145	27.88849999069127
23	14.280527991361494	23.536667101074226	40.874648595312124	21.308156312252155
24	14.419228119822018	24.4898814067358	37.67290972390296	23.41798074953922
25	12.717591644480851	33.760449053301805	31.023215981233594	22.498743320983746
26	16.591421071249044	30.69042876026288	26.920391711503733	25.797758456984344
27	16.999609033194943	27.326717926758885	39.685457896598585	15.988215143447581
28	14.020814328002531	25.176400498948116	34.73833150261576	26.0644536704336
29	16.9195539254929	29.918734756948968	30.09187719918828	23.069834118369855
30	27.66509038780184	23.60089736190494	20.687729227561295	28.046283022731927
31	29.612477426321377	26.476365125760985	23.21830841695679	20.692849030960847
32	22.116154376035595	25.228994842961665	26.743991212555617	25.910859568447115
33	16.024519203917116	26.444249995345633	28.032319922551334	29.498910878185914
34	14.610522592296093	23.51013721073111	32.00621823394709	29.87312196302571
35	29.044644685644073	23.96347252992758	25.84104406754417	21.15083871688418
36	19.970491314951687	26.19058700873159	28.21523653491706	25.623685141399662
37	20.72915309143038	30.81656209856087	24.585295924636494	23.868988885372254
38	15.69312829296446	30.360434159328282	23.740062927038146	30.206374620669113
39	24.01280881723233	23.575763781579877	25.90061996164802	26.510807439539775
40	13.199318600711187	18.266527656247092	29.53102600860127	39.003127734440454
41	20.324223186193286	24.133822352130768	24.249250646956973	31.29270381471897
42	20.187384804423512	23.34816524863627	26.595051477296	29.86939846964422
43	22.974419600469158	28.55547074265076	27.906652020926032	20.56345763595405
44	14.405265019641428	32.44931394634446	27.361160240537675	25.784260793476438
45	21.134548433340157	30.93943738015006	25.738647999553184	22.187366186956602
46	22.49083089754808	21.403570830152848	28.32135609628954	27.78424217600953
47	22.139891646342598	18.13527451454955	31.535661757861227	28.189172081246628
48	18.94187626831493	16.831586394355185	29.86427866624467	34.36225867108521
49	18.893470854355556	25.880606184722506	29.551970658872158	25.673952302049784
50	18.826913410161414	28.635991287025487	25.632528438180703	26.904566864632397
51	17.306331800495226	20.62954964347551	32.875653938525126	29.188464617504145
52	22.647217619570682	19.41848342114572	32.62664531863795	25.307653640645654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	66.0
2	89.0
3	362.0
4	635.0
5	443.5
6	252.0
7	308.0
8	364.0
9	419.5
10	475.0
11	468.0
12	461.0
13	451.5
14	408.0
15	374.0
16	372.0
17	370.0
18	328.0
19	286.0
20	324.0
21	362.0
22	335.5
23	309.0
24	379.0
25	449.0
26	533.5
27	618.0
28	862.0
29	1106.0
30	1249.0
31	1392.0
32	1659.0
33	1926.0
34	2297.5
35	2669.0
36	2899.0
37	3129.0
38	3570.0
39	5052.5
40	6094.0
41	9023.0
42	11952.0
43	17045.0
44	22138.0
45	24866.5
46	27595.0
47	29548.5
48	31502.0
49	30076.0
50	28650.0
51	25131.0
52	21612.0
53	18150.0
54	14688.0
55	11433.5
56	8179.0
57	7040.5
58	5902.0
59	5364.5
60	4827.0
61	4605.5
62	4384.0
63	3827.0
64	2512.0
65	1754.0
66	1379.5
67	1005.0
68	798.5
69	592.0
70	448.5
71	305.0
72	324.5
73	344.0
74	250.5
75	157.0
76	118.5
77	80.0
78	57.0
79	34.0
80	25.5
81	17.0
82	13.0
83	9.0
84	4.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	214852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.08396477575261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63444520081688	21.851320909277085
2	9.370938795717557	5.638299852922011
3	3.0555727458382327	2.757712285666412
4	1.5038059285846896	1.80961778340439
5	1.1185716937929329	1.6825535717610263
6	0.8106937310477134	1.4633328989257721
7	0.7178662045918683	1.511738312885149
8	0.6931121975369764	1.6681250349077505
9	0.6977535738597685	1.8892074544337496
>10	9.149699857664459	52.01068642600488
>50	0.20267343276192834	3.967847634650829
>100	0.038678136023268764	2.2136168152961107
>500	0.004641376322792252	1.0225643698918325
>1k	0.0015471254409307506	0.5133766499730047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1103	0.5133766499730047	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT	988	0.4598514326140785	TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	662	0.3081190773183401	TruSeq Adapter, Index 13 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTTG	547	0.2545938599594139	TruSeq Adapter, Index 13 (95% over 24bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	470	0.2187552361625677	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	420	0.195483402528252	No Hit
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	325	0.15126691862305217	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	309	0.1438199318600711	TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	235	0.10937761808128385	TruSeq Adapter, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	232	0.10798130806322491	TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	226	0.10518868802710704	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4686947293951185	0.0
2	0.0	0.0	0.0	1.5522313034088582	0.0
3	0.0	0.0	0.0	2.45238582838419	0.0
4	0.0	0.0	0.0	3.0230111890976112	0.0
5	0.0	0.0	0.0	5.950607860294529	0.0
6	0.0	0.0	0.0	6.624560162344311	0.0
7	0.0	0.0	0.0	10.440675441699403	0.0
8	0.0	0.0	0.0	14.197214826950646	0.0
9	0.0	0.0	0.0	18.54346247649545	0.0
10	0.0	0.0	0.0	19.757321318861354	0.0
11	0.0	0.0	0.0	20.95907880773742	0.0
12	0.0	0.0	0.0	21.963956584067173	0.0
13	0.0	0.0	0.0	22.27207566138551	0.0
14	0.0	0.0	0.0	22.521549717945376	0.0
15	0.0	0.0	0.0	23.38633105579655	0.0
16	0.0	0.0	0.0	24.24878521028429	0.0
17	0.0	0.0	0.0	25.655800271815018	0.0
18	0.0	0.0	0.0	26.112393647720292	0.0
19	0.0	0.0	0.0	26.754230819354717	0.0
20	0.0	0.0	0.0	27.470537858618957	0.0
21	0.0	0.0	0.0	28.091430379982498	0.0
22	4.654366726863143E-4	0.0	0.0	28.848230409770448	0.0
23	4.654366726863143E-4	0.0	0.0	29.3122707724387	0.0
24	4.654366726863143E-4	0.0	0.0	29.67856943384283	0.0
25	4.654366726863143E-4	0.0	0.0	30.067209055535905	0.0
26	4.654366726863143E-4	0.0	0.0	30.415821123377953	0.0
27	4.654366726863143E-4	0.0	0.0	30.758847951147768	0.0
28	4.654366726863143E-4	0.0	0.0	31.080930128646695	0.0
29	4.654366726863143E-4	0.0	0.0	31.584067171820603	0.0
30	4.654366726863143E-4	0.0	0.0	32.06067432465139	0.0
31	4.654366726863143E-4	0.0	0.0	32.41626794258373	0.0
32	4.654366726863143E-4	0.0	0.0	32.752313220263254	0.0
33	4.654366726863143E-4	0.0	0.0	33.08835849794277	0.0
34	4.654366726863143E-4	0.0	0.0	33.650140561875155	0.0
35	4.654366726863143E-4	0.0	0.0	34.00107981308063	0.0
36	4.654366726863143E-4	0.0	0.0	34.37110196786625	0.0
37	4.654366726863143E-4	0.0	0.0	34.661534451622515	0.0
38	4.654366726863143E-4	0.0	0.0	34.95848304879638	0.0
39	4.654366726863143E-4	0.0	0.0	35.29685550983933	0.0
40	4.654366726863143E-4	0.0	0.0	35.66268873457077	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGGG	35	1.0143776E-7	46.000004	2
CTACGAA	35	1.0143776E-7	46.000004	11
TACGAAT	35	1.0143776E-7	46.000004	12
CGGGCAC	35	1.0143776E-7	46.000004	5
CGGGATG	35	1.0143776E-7	46.000004	5
CGAATAT	35	1.0143776E-7	46.000004	14
GCTACGA	35	1.0143776E-7	46.000004	10
AGCTACG	35	1.0143776E-7	46.000004	9
CTAGCGG	70	0.0	46.000004	1
AGTCCGG	20	6.299947E-4	46.0	1
ACAAAGG	30	1.8542942E-6	46.0	1
ACCGGGT	20	6.299947E-4	46.0	3
ATGATGG	25	3.4078228E-5	46.0	1
AGTCAGG	25	3.4078228E-5	46.0	1
TTTACTC	20	6.299947E-4	46.0	37
TACTTAA	25	3.4078228E-5	46.0	33
CTCCGGG	30	1.8542942E-6	46.0	2
GGATGTT	25	3.4078228E-5	46.0	7
TTTACGG	30	1.8542942E-6	46.0	1
CACGGGA	45	3.074092E-10	46.0	3
>>END_MODULE