FastQCFastQC Report
Thu 26 May 2016
SRR1544922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544922_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences416469
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT17100.4105947861665574TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT11980.2876564642266291TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT9560.22954889799720987TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6860.1647181422867008No Hit
ACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC4680.11237330989821571TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT4330.10396932304685341TruSeq Adapter, Index 14 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4280.10276875349665882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTAGG700.046.0000041
TCGTCGG351.0174699E-746.0000041
TCCGGAC206.3067966E-446.013
TTCTACG206.3067966E-446.043
AACCGGG501.6370905E-1146.02
AATCCGG206.3067966E-446.01
ACGCCAA206.3067966E-446.043
CTCGTCC453.092282E-1046.036
GTACAGG1250.046.01
CAAGTGA206.3067966E-446.027
GAATGCG206.3067966E-446.013
CAACGAG206.3067966E-446.021
TCCGATG206.3067966E-446.022
TCTTCGA206.3067966E-446.039
ACCGCGT301.8585233E-646.010
AACTTAC206.3067966E-446.013
GACCGAT551.8189894E-1246.08
CCGGATA405.5970304E-946.019
TTCACGG253.4133765E-546.01
TCGGGTA253.4133765E-546.04
Produced by FastQC (version 0.11.5)