FastQCFastQC Report
Thu 26 May 2016
SRR1544921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544921_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences263116
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT13930.5294242843460679TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT10110.38424117119445417TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT8080.3070888885510573TruSeq Adapter, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTTG5720.21739460922178813TruSeq Adapter, Index 14 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.16874686450082854No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4200.15962541236564862No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA4010.15240426275863117No Hit
ACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC3530.13416135848827135TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT3370.1280803903981514TruSeq Adapter, Index 14 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC2690.10223627601514161TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAG700.046.00000418
GACCGAT700.046.0000048
TACGGGA351.01556E-746.0000043
TTGACGG351.01556E-746.0000041
AACGCAA700.046.00000417
TTACGGG750.046.0000042
CACACGG351.01556E-746.0000041
CAACTGG351.01556E-746.0000041
ATTGCGG700.046.0000041
CCCGGAC351.01556E-746.00000412
TCGCGGG850.046.0000042
TCACGGG800.046.02
TCACGAC206.302541E-446.024
GTTGAGG501.6370905E-1146.01
ACTATTA206.302541E-446.046
GTCGCAA206.302541E-446.041
AATTTCC206.302541E-446.045
TCCGCCG206.302541E-446.015
ATCTACC206.302541E-446.014
AAATAGG253.4099263E-546.01
Produced by FastQC (version 0.11.5)