FastQCFastQC Report
Thu 26 May 2016
SRR1544920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544920_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1164941
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT40730.3496314405622259TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT28640.24584936061139576TruSeq Adapter, Index 15 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT27040.23211475945992113TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.20971019133157817No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18430.15820543701354833No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13440.11537064967238685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC351.01957994E-746.00000411
ACGGGTA750.046.0000044
CGATACG351.01957994E-746.0000049
TTTAACG253.4171848E-546.031
CGATTCG206.3114904E-446.09
GCGATAT405.6115823E-946.08
TTAACGA206.3114904E-446.032
GCGATAA405.6115823E-946.08
ACGTAGG800.046.01
GGCGATA1950.044.8205157
CGGGTAT900.043.4444435
AACGCAA7400.043.202717
TAGTAGG2400.043.1251
CTACCGG1300.042.461541
CAACGCA7550.042.3443716
CGTTTTT19850.042.292191
CGCACTT6900.042.034
ACCCGCA6900.042.031
ATTGCGG1150.042.01
CCGCACT6900.042.033
Produced by FastQC (version 0.11.5)