##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544920_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1164941
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.968143451041726	34.0	33.0	34.0	31.0	34.0
2	33.27803897364759	34.0	33.0	34.0	31.0	34.0
3	33.33841456348433	34.0	34.0	34.0	31.0	34.0
4	36.600177176354855	37.0	37.0	37.0	35.0	37.0
5	36.60116864287548	37.0	37.0	37.0	35.0	37.0
6	36.67501444279152	37.0	37.0	37.0	35.0	37.0
7	36.50340403505414	37.0	37.0	37.0	35.0	37.0
8	36.533260482719726	37.0	37.0	37.0	35.0	37.0
9	38.34664674004949	39.0	39.0	39.0	37.0	39.0
10	38.197668379772026	39.0	39.0	39.0	35.0	39.0
11	37.68790522438476	39.0	37.0	39.0	35.0	39.0
12	37.622446115296825	39.0	37.0	39.0	35.0	39.0
13	37.60920510137423	39.0	37.0	39.0	35.0	39.0
14	38.97549317948291	41.0	38.0	41.0	35.0	41.0
15	39.02722026265708	41.0	38.0	41.0	35.0	41.0
16	39.00182069306514	41.0	37.0	41.0	35.0	41.0
17	39.055201937265494	41.0	37.0	41.0	35.0	41.0
18	39.04657403250465	41.0	37.0	41.0	35.0	41.0
19	38.981660873812494	41.0	37.0	41.0	35.0	41.0
20	38.89899917678235	41.0	36.0	41.0	35.0	41.0
21	38.84638449500876	41.0	36.0	41.0	35.0	41.0
22	38.8133579297149	41.0	36.0	41.0	35.0	41.0
23	38.79649698997632	41.0	35.0	41.0	35.0	41.0
24	38.735669875126725	41.0	35.0	41.0	35.0	41.0
25	38.69119122771024	41.0	35.0	41.0	35.0	41.0
26	38.63789324952938	41.0	35.0	41.0	35.0	41.0
27	38.590087394983954	41.0	36.0	41.0	35.0	41.0
28	38.61856694888411	41.0	36.0	41.0	35.0	41.0
29	38.614354718393464	41.0	36.0	41.0	35.0	41.0
30	38.54723028891592	40.0	36.0	41.0	35.0	41.0
31	38.48736888820979	40.0	35.0	41.0	35.0	41.0
32	38.39433842572285	40.0	35.0	41.0	35.0	41.0
33	38.29748716887808	40.0	35.0	41.0	35.0	41.0
34	38.21378164216042	40.0	35.0	41.0	35.0	41.0
35	38.092247590221305	40.0	35.0	41.0	35.0	41.0
36	38.05606550031289	40.0	35.0	41.0	35.0	41.0
37	37.97640395522177	40.0	35.0	41.0	35.0	41.0
38	37.896206760685736	40.0	35.0	41.0	35.0	41.0
39	37.802640648753886	40.0	35.0	41.0	35.0	41.0
40	37.72489937258625	40.0	35.0	41.0	35.0	41.0
41	37.68683735914523	40.0	35.0	41.0	35.0	41.0
42	37.616335934609566	40.0	35.0	41.0	35.0	41.0
43	37.53228618445054	39.0	35.0	41.0	35.0	41.0
44	37.45015326956472	39.0	35.0	41.0	34.0	41.0
45	37.38020637955055	39.0	35.0	41.0	34.0	41.0
46	37.38442891099206	39.0	35.0	41.0	35.0	41.0
47	37.32562507457459	39.0	35.0	41.0	34.0	41.0
48	37.32280175562539	39.0	35.0	41.0	34.0	41.0
49	37.26029730261018	39.0	35.0	41.0	34.0	41.0
50	37.18895205851627	39.0	35.0	41.0	34.0	41.0
51	37.09502283806648	39.0	35.0	41.0	34.0	41.0
52	36.87693883209536	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	4.0
14	18.0
15	32.0
16	37.0
17	53.0
18	104.0
19	183.0
20	313.0
21	489.0
22	775.0
23	1226.0
24	2108.0
25	3425.0
26	4905.0
27	5822.0
28	5909.0
29	6484.0
30	7511.0
31	8982.0
32	11338.0
33	16297.0
34	51110.0
35	190361.0
36	45800.0
37	73902.0
38	129205.0
39	593678.0
40	4865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.329686224452566	21.40700687845994	22.933779478960737	20.329527418126755
2	28.275337549283613	23.693989652694857	25.416051113318183	22.614621684703344
3	28.56891464889638	24.622620373048935	24.162768758246127	22.645696219808556
4	26.36399611654153	26.352493388077164	21.5073553081229	25.77615518725841
5	24.298569627131332	36.092385794645395	20.798821571221204	18.810223007002072
6	87.66933260997767	3.192693878917473	6.6502080362868154	2.4877654748180382
7	86.99977080384328	3.133978458994919	5.64440602571289	4.221844711448906
8	76.37322405169017	5.727156997650525	10.906045885585621	6.993573065073681
9	47.57451235727818	22.889571231504426	14.736883670503484	14.799032740713908
10	36.81336651384062	24.24895338047163	23.78026011617756	15.157419989510199
11	36.307589826437564	19.021306658448797	24.837566881069513	19.833536634044126
12	23.274397587517306	28.978463286981913	27.803038952187276	19.944100173313497
13	16.888408940881984	31.733881801739315	29.146797992344673	22.230911265034024
14	16.331556705446886	25.571595471358638	38.651399512936706	19.445448310257774
15	19.037874021087763	18.914777658267674	41.53068696182897	20.516661358815597
16	18.768847521033255	21.05634534281135	29.376766720374683	30.798040415780715
17	20.545332338719298	23.418181693321806	29.956023523938125	26.080462444020768
18	24.124826922565177	28.041505964679757	26.682209656969754	21.151457455785312
19	28.36821778957046	23.558102942552456	24.38896047095947	23.68471879691761
20	24.836708468497545	27.940642487473617	25.413647558116676	21.809001485912162
21	21.577315932738227	24.31736886245741	24.958860577488473	29.146454627315887
22	21.470958615071492	28.708320850583853	23.490460031881444	26.330260502463215
23	21.277729945121685	22.952235349258032	32.7486113030617	23.021423402558586
24	21.689596297151528	22.359329785800313	30.3383604834923	25.61271343355586
25	19.58202175045775	28.712012024643307	27.956351437540615	23.749614787358333
26	20.186344201122633	27.786299907033918	26.955785743655685	25.07157014818776
27	21.525381972134213	27.5462877519119	31.155655093262236	19.772675182691657
28	19.716706682999398	25.734436336260806	27.757199720844234	26.79165725989557
29	22.124553947367293	25.28892021140985	28.305897036845646	24.280628804377216
30	25.024099932957977	27.06617760041066	22.450664883457616	25.45905758317374
31	26.497479271482415	25.364117152714172	24.279169503004873	23.859234072798536
32	24.437289098761227	26.774574849713417	24.364066506372424	24.42406954515293
33	19.0734981428244	29.47797356260961	26.238925404805908	25.20960288976008
34	22.08386519145605	24.985728890991048	26.16879309767619	26.761612819876714
35	24.31135997445364	25.588677881540782	24.97019162343844	25.129770520567135
36	23.366505256489383	30.301191219126117	23.729098726888314	22.603204797496183
37	22.65531044061459	27.840294057810656	26.644782868831985	22.859612632742774
38	22.302159508507298	26.80255909955955	25.008991871691354	25.886289520241796
39	25.223165808397162	24.68159331674308	28.09550011545649	21.999740759403267
40	17.519513863792245	24.88623887390005	31.071788184981042	26.522459077326666
41	21.62264011653809	27.07261569470042	24.89516636464851	26.40957782411298
42	22.402078731884277	23.566000338214554	27.244899097894226	26.787021832006943
43	22.804073339336497	28.112067478095458	26.00483629643046	23.079022886137583
44	20.307294532512802	27.316147341367504	26.117460025872557	26.259098100247137
45	22.497963416173008	26.866768359942693	25.70868395910179	24.92658426478251
46	23.864041183201554	24.268181822083694	27.68037179565317	24.187405199061583
47	22.373665275752163	21.836556529472308	30.399050252330373	25.390727942445153
48	20.983294432936948	22.590328608916675	27.351685621846944	29.074691336299434
49	20.551512909237463	24.912077092316263	30.010704404772433	24.525705593673845
50	20.70242183938929	25.360511819911906	27.295030392097107	26.642035948601688
51	20.804572935453383	23.092242439745874	28.9946014433349	27.108583181465846
52	22.375982989696475	20.626967374313377	31.127155795872923	25.869893840117225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	136.0
1	378.5
2	621.0
3	2664.0
4	4707.0
5	3153.5
6	1600.0
7	1729.5
8	1859.0
9	1849.0
10	1839.0
11	1794.5
12	1750.0
13	1747.5
14	1657.5
15	1570.0
16	1552.5
17	1535.0
18	1585.5
19	1636.0
20	1714.0
21	1792.0
22	2185.5
23	2579.0
24	3032.5
25	3486.0
26	4267.0
27	5048.0
28	7008.5
29	8969.0
30	10253.0
31	11537.0
32	12948.0
33	14359.0
34	15764.5
35	17170.0
36	18941.0
37	20712.0
38	22891.5
39	30585.0
40	36099.0
41	45939.0
42	55779.0
43	75803.0
44	95827.0
45	109947.0
46	124067.0
47	124409.5
48	124752.0
49	113167.0
50	101582.0
51	92614.0
52	83646.0
53	76513.0
54	69380.0
55	63227.5
56	57075.0
57	54419.5
58	51764.0
59	51358.0
60	50952.0
61	52881.5
62	54811.0
63	50228.5
64	37769.0
65	29892.0
66	23274.0
67	16656.0
68	14232.0
69	11808.0
70	9900.0
71	7992.0
72	6793.0
73	5594.0
74	5292.0
75	4990.0
76	4333.0
77	3676.0
78	2691.0
79	1706.0
80	1290.0
81	874.0
82	680.5
83	487.0
84	294.5
85	102.0
86	59.5
87	17.0
88	22.5
89	16.0
90	4.0
91	4.0
92	4.0
93	3.5
94	3.0
95	5.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1164941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.954096262024333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.76053318055929	16.24244090178725
2	11.560301796666707	5.307125605174809
3	4.210204038186985	2.899242863259229
4	1.9922386872981435	1.829201544206823
5	1.1734528498200978	1.3467774836858655
6	0.7612075832081407	1.0483699284205534
7	0.5779803447025655	0.9286911528902486
8	0.42804557181900854	0.7860319408053419
9	0.3728016858858831	0.7701593206022566
>10	6.694263265448097	41.26954477188317
>50	1.2989981212312274	18.69982239375453
>100	0.1575907451942872	5.858906294635548
>500	0.009755617559646176	1.6077412136824232
>1k	0.00262651241990474	1.4059445852119716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	4073	0.3496314405622259	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT	2864	0.24584936061139576	TruSeq Adapter, Index 15 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	2704	0.23211475945992113	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2443	0.20971019133157817	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1843	0.15820543701354833	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1344	0.11537064967238685	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25082815352880533	0.0
2	0.0	0.0	0.0	0.9817664585588455	0.0
3	0.0	0.0	0.0	1.5383611702223545	0.0
4	8.58412571967164E-5	0.0	0.0	1.9092812425693662	0.0
5	8.58412571967164E-5	0.0	0.0	3.9764245571234937	0.0
6	8.58412571967164E-5	0.0	0.0	4.372066911543159	0.0
7	8.58412571967164E-5	0.0	0.0	6.179197058048433	0.0
8	8.58412571967164E-5	0.0	0.0	8.151657465914584	0.0
9	8.58412571967164E-5	0.0	0.0	9.948400820299053	0.0
10	8.58412571967164E-5	0.0	0.0	10.818745327016561	0.0
11	8.58412571967164E-5	0.0	0.0	11.659388758744004	0.0
12	8.58412571967164E-5	0.0	0.0	12.38474738205626	0.0
13	8.58412571967164E-5	0.0	0.0	12.679526259269783	0.0
14	8.58412571967164E-5	0.0	0.0	12.882369150025623	0.0
15	8.58412571967164E-5	0.0	0.0	13.609444598481812	0.0
16	8.58412571967164E-5	0.0	0.0	14.35875293255195	0.0
17	8.58412571967164E-5	0.0	0.0	15.340433549853598	0.0
18	8.58412571967164E-5	0.0	0.0	15.815479067180226	0.0
19	8.58412571967164E-5	0.0	0.0	16.383919872336882	0.0
20	8.58412571967164E-5	0.0	0.0	17.041635584978124	0.0
21	8.58412571967164E-5	0.0	0.0	17.647331495758156	0.0
22	8.58412571967164E-5	0.0	0.0	18.309510953773625	0.0
23	8.58412571967164E-5	0.0	0.0	18.809622118201695	0.0
24	8.58412571967164E-5	0.0	0.0	19.25239132282236	0.0
25	8.58412571967164E-5	0.0	0.0	19.641681424209466	0.0
26	8.58412571967164E-5	0.0	0.0	20.028567970395066	0.0
27	8.58412571967164E-5	0.0	0.0	20.409703152348488	0.0
28	8.58412571967164E-5	0.0	0.0	20.8145305212882	0.0
29	8.58412571967164E-5	0.0	0.0	21.317131082174978	0.0
30	8.58412571967164E-5	0.0	0.0	21.778613680864524	0.0
31	8.58412571967164E-5	0.0	0.0	22.21769171142573	0.0
32	8.58412571967164E-5	0.0	0.0	22.614106637160166	0.0
33	8.58412571967164E-5	0.0	0.0	22.997130326771913	0.0
34	8.58412571967164E-5	0.0	0.0	23.488571524223115	0.0
35	8.58412571967164E-5	0.0	0.0	23.917262762663515	0.0
36	8.58412571967164E-5	0.0	0.0	24.38793037587311	0.0
37	8.58412571967164E-5	0.0	0.0	24.80262948939045	0.0
38	8.58412571967164E-5	0.0	0.0	25.204881620614263	0.0
39	8.58412571967164E-5	0.0	0.0	25.660269490042843	0.0
40	8.58412571967164E-5	0.0	0.0	26.147246942119814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGC	35	1.01957994E-7	46.000004	11
ACGGGTA	75	0.0	46.000004	4
CGATACG	35	1.01957994E-7	46.000004	9
TTTAACG	25	3.4171848E-5	46.0	31
CGATTCG	20	6.3114904E-4	46.0	9
GCGATAT	40	5.6115823E-9	46.0	8
TTAACGA	20	6.3114904E-4	46.0	32
GCGATAA	40	5.6115823E-9	46.0	8
ACGTAGG	80	0.0	46.0	1
GGCGATA	195	0.0	44.820515	7
CGGGTAT	90	0.0	43.444443	5
AACGCAA	740	0.0	43.2027	17
TAGTAGG	240	0.0	43.125	1
CTACCGG	130	0.0	42.46154	1
CAACGCA	755	0.0	42.34437	16
CGTTTTT	1985	0.0	42.29219	1
CGCACTT	690	0.0	42.0	34
ACCCGCA	690	0.0	42.0	31
ATTGCGG	115	0.0	42.0	1
CCGCACT	690	0.0	42.0	33
>>END_MODULE