FastQCFastQC Report
Thu 26 May 2016
SRR1544919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544919_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences730332
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT31210.4273398947328064TruSeq Adapter, Index 21 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA23440.32094992414408785No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT23270.31862221564986887TruSeq Adapter, Index 15 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT22060.3020544081321919TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15610.21373840938093908No Hit
TGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTTG13670.18717514774102736TruSeq Adapter, Index 21 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11530.15787340551968146No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7970.10912845117015275No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC7530.10310379389099752TruSeq Adapter, Index 15 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC7490.10255609777471068TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT453.092282E-1046.03
ACGGGTC501.6370905E-1146.04
ACGGGTA501.6370905E-1146.04
ACGGGAT1150.046.04
ATCGTCA206.3099357E-446.09
TAACCGG206.3099357E-446.01
GCGATAT206.3099357E-446.08
GCTAGAT206.3099357E-446.027
TCTAATC351.0188887E-745.99999633
CGGGATA1400.044.357145
GGCGATA1150.044.07
TAGTAGG1150.044.01
TACGGGA1100.043.9090923
CGTTTTT11850.043.6708871
TTACGGG1900.043.578952
CGAATAT850.043.29411714
AGTGCGC1600.043.12512
CGGTCTA800.043.12530
CGCAATC750.042.9333319
ACGCGCA750.042.9333316
Produced by FastQC (version 0.11.5)