##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544919_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	730332
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93271827059474	34.0	33.0	34.0	31.0	34.0
2	33.28867966897247	34.0	34.0	34.0	31.0	34.0
3	33.41423078818948	34.0	34.0	34.0	31.0	34.0
4	36.62206092571598	37.0	37.0	37.0	35.0	37.0
5	36.6335337901119	37.0	37.0	37.0	35.0	37.0
6	36.42870639654294	37.0	37.0	37.0	35.0	37.0
7	36.2388050913831	37.0	37.0	37.0	35.0	37.0
8	36.381289605275406	37.0	37.0	37.0	35.0	37.0
9	38.27686312526358	39.0	39.0	39.0	37.0	39.0
10	38.20217380588554	39.0	39.0	39.0	35.0	39.0
11	37.73546688355433	39.0	37.0	39.0	35.0	39.0
12	37.716935585459765	39.0	37.0	39.0	35.0	39.0
13	37.708748897761566	39.0	37.0	39.0	35.0	39.0
14	39.00533319093234	41.0	38.0	41.0	35.0	41.0
15	39.027401236697834	41.0	38.0	41.0	35.0	41.0
16	39.020890499115474	41.0	38.0	41.0	35.0	41.0
17	39.10547121035365	41.0	38.0	41.0	35.0	41.0
18	39.12110519599305	41.0	38.0	41.0	35.0	41.0
19	39.090798431398326	41.0	37.0	41.0	35.0	41.0
20	39.024228706944236	41.0	37.0	41.0	35.0	41.0
21	38.96657273678272	41.0	37.0	41.0	35.0	41.0
22	38.94125822228795	41.0	37.0	41.0	35.0	41.0
23	38.90499526242859	41.0	36.0	41.0	35.0	41.0
24	38.85504126890236	41.0	36.0	41.0	35.0	41.0
25	38.80898824096438	41.0	36.0	41.0	35.0	41.0
26	38.73601047194974	41.0	36.0	41.0	35.0	41.0
27	38.80078238390212	41.0	36.0	41.0	35.0	41.0
28	38.81347387215677	41.0	36.0	41.0	35.0	41.0
29	38.81801016523992	41.0	36.0	41.0	35.0	41.0
30	38.76250390233483	41.0	36.0	41.0	35.0	41.0
31	38.701390600439254	41.0	36.0	41.0	35.0	41.0
32	38.62760634889338	41.0	36.0	41.0	35.0	41.0
33	38.536995503414886	41.0	36.0	41.0	35.0	41.0
34	38.44372970101269	41.0	36.0	41.0	35.0	41.0
35	38.330518175295616	40.0	35.0	41.0	35.0	41.0
36	38.307297229205346	40.0	35.0	41.0	35.0	41.0
37	38.240297563299976	40.0	35.0	41.0	35.0	41.0
38	38.158108914849684	40.0	35.0	41.0	35.0	41.0
39	38.09989429464956	40.0	35.0	41.0	35.0	41.0
40	38.0339996056588	40.0	35.0	41.0	35.0	41.0
41	37.99718894968316	40.0	35.0	41.0	35.0	41.0
42	37.93578263036537	40.0	35.0	41.0	35.0	41.0
43	37.87306594808936	40.0	35.0	41.0	35.0	41.0
44	37.73732768110941	40.0	35.0	41.0	35.0	41.0
45	37.7100565222392	40.0	35.0	41.0	35.0	41.0
46	37.631982440862515	40.0	35.0	41.0	35.0	41.0
47	37.54930908134931	39.0	35.0	41.0	35.0	41.0
48	37.48989774513509	39.0	35.0	41.0	35.0	41.0
49	37.43375478549481	39.0	35.0	41.0	35.0	41.0
50	37.37520880914433	39.0	35.0	41.0	35.0	41.0
51	37.28573854082801	39.0	35.0	41.0	35.0	41.0
52	36.8028499367411	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	3.0
14	2.0
15	13.0
16	21.0
17	31.0
18	62.0
19	76.0
20	166.0
21	268.0
22	423.0
23	732.0
24	1183.0
25	1872.0
26	2922.0
27	3224.0
28	3237.0
29	3326.0
30	3744.0
31	4628.0
32	5893.0
33	8788.0
34	30293.0
35	114184.0
36	27625.0
37	45815.0
38	82597.0
39	386801.0
40	2401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.541140193774886	21.28662033157523	22.023682380068244	22.14855709458164
2	28.14226954316667	23.813005591977348	25.354633235295726	22.690091629560253
3	28.614383595405922	24.328661485461406	24.36891715000849	22.68803776912418
4	26.259974915517876	25.829485768116417	22.028200873027608	25.8823384433381
5	24.366041745397986	35.36638131699008	21.055903342589396	19.21167359502254
6	86.38468532119639	3.1278925201141403	7.334335617226138	3.1530865414633347
7	85.63379394576714	3.049982747572337	6.339445621991094	4.976777684669438
8	74.37946030024702	5.7928448979368286	12.020423588176337	7.807271213639824
9	46.894836868711764	22.22906842367581	15.222665856076414	15.653428851536013
10	35.8735479206717	23.575716249596073	25.06818816647771	15.48254766325452
11	35.167156854690745	18.781458295679226	25.190598248467822	20.86078660116221
12	22.76909679433463	27.892930886227084	28.72529205895401	20.61268026048427
13	16.83727400689002	30.514615270863114	29.792341017509845	22.855769704737025
14	16.275063943521577	25.041214132750582	38.272730758066196	20.410991165661642
15	18.929199323047598	18.53431042320479	41.24754221367816	21.288948040069446
16	18.41751422640662	21.57991160184683	29.64720154669383	30.355372625052716
17	20.105102884715446	23.388267253796904	30.22680096175438	26.27982889973327
18	23.591051740852105	28.219357771534042	26.883800791968582	21.30578969564527
19	27.494208113570267	23.387445709622472	24.83185729229994	24.28648888450732
20	24.077268968085747	28.300553720773564	25.61588428276455	22.006293028376138
21	21.204876686219418	24.10725533045245	25.123779322280825	29.564088661047304
22	21.17530109593993	28.730358248029663	23.728934238127316	26.365406417903092
23	20.792872282742643	22.96051658697688	32.828083666058724	23.41852746422175
24	21.805973173844222	22.40611119326553	29.951583663320243	25.836331969570004
25	19.321897438425264	28.44966398843266	28.264542701127702	23.96389587201437
26	20.091684329866418	26.826977319903822	27.108219275617117	25.973119074612644
27	21.762431332599423	26.914334850451578	31.131321097802093	20.19191271914691
28	19.97817430976597	25.512780488873553	27.390693547592054	27.118351653768425
29	22.37010017361967	24.692605554734012	28.139804910643377	24.797489361002942
30	24.74121358505447	27.10808235158805	22.059830323743174	26.090873739614313
31	26.45892552975907	24.7909170076075	23.881056834425987	24.86910062820745
32	23.655953730632095	26.486994955718767	24.635097462523895	25.22195385112524
33	18.518427235832473	29.334330140264974	26.609268113679807	25.53797451022275
34	22.38858491754435	24.619761971267863	25.945323496711087	27.046329614476704
35	23.650339845440154	25.35545477947016	25.380648800819355	25.61355657427033
36	23.63158125345733	30.05947979822875	23.063346532809735	23.24559241550418
37	22.652026749478317	27.746011403033137	26.838342014316773	22.763619833171763
38	22.358461631148575	26.583800244272464	24.58703712831972	26.470700996259232
39	25.31780067147544	24.337424623321997	28.108312383956886	22.236462321245682
40	17.456170618294145	24.60196184748854	31.096131622330663	26.84573591188665
41	21.624411911295137	26.91296561016086	24.878137614126178	26.584484864417828
42	21.828428714611984	23.23368002497494	27.37042879128944	27.567462469123633
43	23.36485324482564	27.624285941188393	25.82469342709891	23.18616738688706
44	20.44809757754008	26.319399944134997	26.705662630146286	26.526839848178636
45	22.448831490335902	26.112918508294857	25.97804833965922	25.46020166171002
46	23.896118477623876	23.204789054840813	28.017120980595127	24.881971486940184
47	22.05298412228959	21.352754637616865	30.742593779267512	25.851667460826032
48	20.809166242202178	21.883335250269738	27.144914915408336	30.162583592119745
49	20.450836058121514	23.720581872353943	30.359206497866726	25.46937557165782
50	20.761653604114294	24.40752972620671	27.76600231127761	27.064814358401385
51	20.884748306249758	22.130209274686035	29.15879353499504	27.826248884069166
52	21.998488358719047	20.232716079810277	31.729131408729184	26.039664152741494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	265.0
2	453.0
3	1734.0
4	3015.0
5	2061.0
6	1107.0
7	1139.5
8	1172.0
9	1197.0
10	1222.0
11	1185.0
12	1148.0
13	1148.5
14	1105.5
15	1062.0
16	1026.5
17	991.0
18	1009.0
19	1027.0
20	1103.0
21	1179.0
22	1330.5
23	1482.0
24	1746.5
25	2011.0
26	2430.0
27	2849.0
28	3822.0
29	4795.0
30	5548.5
31	6302.0
32	7107.0
33	7912.0
34	8629.5
35	9347.0
36	10448.0
37	11549.0
38	12941.0
39	17385.5
40	20438.0
41	26351.0
42	32264.0
43	45282.0
44	58300.0
45	69135.5
46	79971.0
47	81575.5
48	83180.0
49	75323.0
50	67466.0
51	60835.5
52	54205.0
53	49578.5
54	44952.0
55	40455.5
56	35959.0
57	34034.5
58	32110.0
59	31573.0
60	31036.0
61	32331.0
62	33626.0
63	31843.0
64	24199.0
65	18338.0
66	14432.0
67	10526.0
68	9109.0
69	7692.0
70	6394.5
71	5097.0
72	4310.0
73	3523.0
74	3307.0
75	3091.0
76	2722.0
77	2353.0
78	1694.5
79	1036.0
80	787.5
81	539.0
82	414.5
83	290.0
84	171.5
85	53.0
86	36.0
87	19.0
88	17.5
89	8.5
90	1.0
91	2.5
92	4.0
93	3.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	730332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.785816441916957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92977232132853	19.986274504293135
2	10.373963209691219	5.7649807503935975
3	3.6011334934269557	3.001813026936017
4	1.7288825078251313	1.9215364804828063
5	1.078566387379318	1.4984423830071614
6	0.8006470793813718	1.3347979669468628
7	0.6522284456056177	1.2685889907456156
8	0.5586385657723342	1.2417782916740665
9	0.505894249516341	1.2651016280474148
>10	8.419789493236587	50.97459323131525
>50	0.26397544120031513	4.896167113487031
>100	0.07810509308877864	4.001846738786769
>500	0.004943360322074598	0.91025358976969
>1k	0.003460352225452218	1.9338253041146016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	3121	0.4273398947328064	TruSeq Adapter, Index 21 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2344	0.32094992414408785	No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT	2327	0.31862221564986887	TruSeq Adapter, Index 15 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	2206	0.3020544081321919	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1561	0.21373840938093908	No Hit
TGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTTG	1367	0.18717514774102736	TruSeq Adapter, Index 21 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1153	0.15787340551968146	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	797	0.10912845117015275	No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	753	0.10310379389099752	TruSeq Adapter, Index 15 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	749	0.10255609777471068	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32574226516159777	0.0
2	0.0	0.0	0.0	1.2282085407732373	0.0
3	0.0	0.0	0.0	1.9028332320095518	0.0
4	0.0	0.0	0.0	2.3471517063472502	0.0
5	0.0	0.0	0.0	4.755782301747698	0.0
6	0.0	0.0	0.0	5.216805507632145	0.0
7	0.0	0.0	0.0	7.36254196721491	0.0
8	0.0	0.0	0.0	9.59919598210129	0.0
9	0.0	0.0	0.0	11.760131008911015	0.0
10	0.0	0.0	0.0	12.80540904684445	0.0
11	0.0	0.0	0.0	13.756620276805616	0.0
12	0.0	0.0	0.0	14.61280623059102	0.0
13	0.0	0.0	0.0	14.961825580694807	0.0
14	0.0	0.0	0.0	15.210205769430889	0.0
15	0.0	0.0	0.0	16.038869993372877	0.0
16	0.0	0.0	0.0	16.87930968381503	0.0
17	0.0	0.0	0.0	17.932392391405553	0.0
18	0.0	0.0	0.0	18.455579106488557	0.0
19	0.0	0.0	0.0	19.07023107299146	0.0
20	1.3692402907170985E-4	0.0	0.0	19.78606989697836	0.0
21	1.3692402907170985E-4	0.0	0.0	20.4513837542378	0.0
22	1.3692402907170985E-4	0.0	0.0	21.174342627736426	0.0
23	1.3692402907170985E-4	0.0	0.0	21.708894037232383	0.0
24	1.3692402907170985E-4	0.0	0.0	22.191825087768304	0.0
25	1.3692402907170985E-4	0.0	0.0	22.625874259925624	0.0
26	1.3692402907170985E-4	0.0	0.0	23.050338750047924	0.0
27	1.3692402907170985E-4	0.0	0.0	23.46809396274571	0.0
28	1.3692402907170985E-4	0.0	0.0	23.9066616278624	0.0
29	1.3692402907170985E-4	0.0	0.0	24.408762042468357	0.0
30	1.3692402907170985E-4	0.0	0.0	24.904427027707946	0.0
31	1.3692402907170985E-4	0.0	0.0	25.354496311266658	0.0
32	2.738480581434197E-4	0.0	0.0	25.77909772541803	0.0
33	2.738480581434197E-4	0.0	0.0	26.180011282539997	0.0
34	2.738480581434197E-4	0.0	0.0	26.693613315587978	0.0
35	2.738480581434197E-4	0.0	0.0	27.1349194612861	0.0
36	2.738480581434197E-4	0.0	0.0	27.598818071781054	0.0
37	2.738480581434197E-4	0.0	0.0	28.02369333399057	0.0
38	2.738480581434197E-4	0.0	0.0	28.435560813438272	0.0
39	2.738480581434197E-4	0.0	0.0	28.88932704578192	0.0
40	2.738480581434197E-4	0.0	0.0	29.364864198747966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	45	3.092282E-10	46.0	3
ACGGGTC	50	1.6370905E-11	46.0	4
ACGGGTA	50	1.6370905E-11	46.0	4
ACGGGAT	115	0.0	46.0	4
ATCGTCA	20	6.3099357E-4	46.0	9
TAACCGG	20	6.3099357E-4	46.0	1
GCGATAT	20	6.3099357E-4	46.0	8
GCTAGAT	20	6.3099357E-4	46.0	27
TCTAATC	35	1.0188887E-7	45.999996	33
CGGGATA	140	0.0	44.35714	5
GGCGATA	115	0.0	44.0	7
TAGTAGG	115	0.0	44.0	1
TACGGGA	110	0.0	43.909092	3
CGTTTTT	1185	0.0	43.670887	1
TTACGGG	190	0.0	43.57895	2
CGAATAT	85	0.0	43.294117	14
AGTGCGC	160	0.0	43.125	12
CGGTCTA	80	0.0	43.125	30
CGCAATC	75	0.0	42.93333	19
ACGCGCA	75	0.0	42.93333	16
>>END_MODULE