FastQCFastQC Report
Thu 26 May 2016
SRR1544918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544918_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences709538
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT20060.2827191778312085TruSeq Adapter, Index 22 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT19430.27384016078067697Illumina Single End Adapter 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.22592165606352302No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT15900.22408947794198478TruSeq Adapter, Index 22 (96% over 25bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11450.16137261147394502No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA9970.14051396824412504No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9790.13797710622968748No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA9500.1338899396508714No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8940.125997480050399No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG8870.12501092260033994No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT7610.107252888499277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGT351.01883415E-746.00000420
ATTACGG351.01883415E-746.0000041
TTCACGG551.8189894E-1246.0000041
CCGTAGG351.01883415E-746.0000041
AACCGCA206.309814E-446.022
AATCCGC206.309814E-446.039
TACCCGT301.8603878E-646.035
CCGATTT206.309814E-446.011
CAACGAC253.4158256E-546.011
CTATCGG206.309814E-446.01
GTCCTAG253.4158256E-546.042
ATAGCGG900.046.01
CACGATT206.309814E-446.038
TACGGAC206.309814E-446.016
GCCGATG301.8603878E-646.08
GGTATAC206.309814E-446.07
AACGACA206.309814E-446.024
GCGATTG600.046.08
GCCTATA206.309814E-446.012
AGTTACC206.309814E-446.021
Produced by FastQC (version 0.11.5)