##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544918_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	709538
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.907263599694446	34.0	33.0	34.0	31.0	34.0
2	33.23047532337944	34.0	33.0	34.0	31.0	34.0
3	33.29497504009651	34.0	34.0	34.0	31.0	34.0
4	36.58054255022282	37.0	37.0	37.0	35.0	37.0
5	36.58431824652097	37.0	37.0	37.0	35.0	37.0
6	36.66052980953804	37.0	37.0	37.0	35.0	37.0
7	36.465322505630425	37.0	37.0	37.0	35.0	37.0
8	36.49717844569283	37.0	37.0	37.0	35.0	37.0
9	38.310954452051895	39.0	39.0	39.0	37.0	39.0
10	38.132972441222314	39.0	39.0	39.0	35.0	39.0
11	37.55922163435926	39.0	35.0	39.0	35.0	39.0
12	37.48396421333318	39.0	35.0	39.0	35.0	39.0
13	37.46985221369398	39.0	35.0	39.0	35.0	39.0
14	38.81325313091054	41.0	36.0	41.0	35.0	41.0
15	38.86353091730112	41.0	36.0	41.0	35.0	41.0
16	38.864227144987304	41.0	36.0	41.0	35.0	41.0
17	38.92743024334144	41.0	36.0	41.0	35.0	41.0
18	38.890831780679825	41.0	36.0	41.0	35.0	41.0
19	38.819591057843276	41.0	36.0	41.0	35.0	41.0
20	38.75148195022677	41.0	35.0	41.0	35.0	41.0
21	38.69347660026665	41.0	35.0	41.0	35.0	41.0
22	38.65316868159281	41.0	35.0	41.0	35.0	41.0
23	38.63230017278849	41.0	35.0	41.0	35.0	41.0
24	38.57009349745891	41.0	35.0	41.0	35.0	41.0
25	38.52752354348886	40.0	35.0	41.0	35.0	41.0
26	38.469752148581186	40.0	35.0	41.0	35.0	41.0
27	38.45510177044781	40.0	35.0	41.0	35.0	41.0
28	38.47435232503403	40.0	36.0	41.0	35.0	41.0
29	38.4798840935933	40.0	36.0	41.0	35.0	41.0
30	38.40139217349881	40.0	35.0	41.0	35.0	41.0
31	38.304307873574075	40.0	35.0	41.0	35.0	41.0
32	38.233030507175094	40.0	35.0	41.0	35.0	41.0
33	38.14004605813924	40.0	35.0	41.0	35.0	41.0
34	38.01810755731194	40.0	35.0	41.0	35.0	41.0
35	37.9590226316279	40.0	35.0	41.0	35.0	41.0
36	37.895088071392934	40.0	35.0	41.0	35.0	41.0
37	37.83388345655906	40.0	35.0	41.0	35.0	41.0
38	37.74721297520358	40.0	35.0	41.0	35.0	41.0
39	37.660152098971444	40.0	35.0	41.0	35.0	41.0
40	37.58475655990236	40.0	35.0	41.0	34.0	41.0
41	37.53847433118452	40.0	35.0	41.0	34.0	41.0
42	37.45587410399443	39.0	35.0	41.0	34.0	41.0
43	37.362579594045705	39.0	35.0	41.0	34.0	41.0
44	37.27397405072033	39.0	35.0	41.0	34.0	41.0
45	37.21351499144514	39.0	35.0	41.0	34.0	41.0
46	37.223938675588904	39.0	35.0	41.0	34.0	41.0
47	37.169343995670424	39.0	35.0	41.0	34.0	41.0
48	37.1605833655139	39.0	35.0	41.0	34.0	41.0
49	37.08787267207676	39.0	35.0	41.0	34.0	41.0
50	37.00252558707215	38.0	35.0	41.0	34.0	41.0
51	36.92331488940691	38.0	35.0	41.0	34.0	41.0
52	36.72967762121267	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	3.0
15	3.0
16	13.0
17	27.0
18	56.0
19	96.0
20	197.0
21	292.0
22	462.0
23	743.0
24	1296.0
25	2088.0
26	3108.0
27	3542.0
28	3690.0
29	4175.0
30	4805.0
31	6012.0
32	7729.0
33	10996.0
34	33629.0
35	129261.0
36	28852.0
37	45587.0
38	78093.0
39	341786.0
40	2993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.90572457007235	22.602735864745792	23.402411146407946	20.089128418773907
2	28.10067959714631	24.10794065997875	25.495040434761773	22.29633930811317
3	27.88180478001178	24.971601239116158	24.59346786218638	22.55312611868568
4	25.972675177368938	27.65588312394826	21.597997570249937	24.773444128432867
5	24.13330928012312	36.36901758609123	20.22315929520336	19.274513838582287
6	87.21294701622746	3.440125828355916	6.776381250898472	2.570545904518151
7	86.16635049849339	3.564432067063357	5.56051966208998	4.708697772353278
8	75.06645169110041	6.058167427255482	11.476059069422638	7.399321812221474
9	49.70262339719649	20.876683137478192	14.722960574345558	14.697732890979765
10	38.93308039879471	21.66874783309703	24.47804064052947	14.92013112757879
11	37.87661830656005	17.051236156484926	25.536898657999995	19.535246878955036
12	22.3625795940457	29.611521863522462	28.744478801699135	19.2814197407327
13	15.905420146630625	32.03492977120324	29.61067624285098	22.44897383931516
14	15.519817120436114	23.503321879871127	40.962006263230435	20.01485473646232
15	17.27659406543413	17.881776592656067	42.90369226172523	21.93793708018457
16	17.36721641406098	21.026780806665744	29.06229123739673	32.54371154187655
17	20.247259484340514	23.06345819392337	31.601971987405893	25.087310334330226
18	23.535173591830176	27.312702068106287	26.501892780936327	22.650231559127207
19	26.566441825525906	23.139282180799338	25.580307185802592	24.713968807872163
20	22.20938131572939	29.014231795900997	26.35320447953457	22.423182408835043
21	21.39011582184464	24.78105471447618	24.765692605610976	29.063136858068205
22	20.599883304347337	28.511369369928037	23.579568677082836	27.30917864864179
23	19.95890283536611	22.600762749845675	34.615764060557716	22.8245703542305
24	20.078135350044676	22.27238005575459	32.09059979874228	25.558884795458454
25	18.376887495807132	27.896039394648348	28.815651874881965	24.911421234662555
26	19.530032218147582	27.50522170764638	27.71874092719488	25.246005147011154
27	21.727095659429093	26.205784609140032	32.54173842697642	19.52538130445445
28	19.71071316828697	25.358331759539304	28.90218705693	26.02876801524372
29	21.27299735884477	26.315292486096588	27.940716353458168	24.47099380160048
30	28.30151450662262	23.895126124323152	22.4276923857496	25.37566698330463
31	27.373586756452788	25.349734616045932	25.05094864545662	22.225729982044655
32	26.193664046182164	24.858288069137945	24.714955365322226	24.233092519357665
33	22.770309694477252	25.236280509289145	26.673412840468025	25.319996955765582
34	22.003331745445628	24.036908523574496	28.08390812049531	25.875851610484567
35	29.72286191860055	23.283178631729378	23.19171066243105	23.80224878723902
36	23.757430891650625	25.33113096127339	26.103182634333887	24.808255512742093
37	25.327184731473157	25.325493490130196	26.962192299778167	22.38512947861848
38	23.150134312749987	24.440974267762964	25.319151335094105	27.089740084392943
39	27.557227378942358	20.684727245052414	26.89440170928125	24.86364366672398
40	19.42616181233422	19.657861876319522	30.746344804647528	30.169631506698725
41	22.610910197903426	24.307366201669257	25.246005147011154	27.835718453416163
42	24.092437614334962	21.808275243891096	27.003063965566326	27.09622317620762
43	23.80210785046044	26.587723279091463	25.900938357071784	23.70923051337631
44	19.930856416428718	27.41586779002675	27.5506033503491	25.102672443195434
45	23.96023891602705	25.502510084026508	26.503302148722124	24.033948851224316
46	24.54273062189763	22.706888144116313	27.837127821201967	24.91325341278409
47	24.274668869038727	20.305043563558257	29.906079730754374	25.514207836648634
48	22.358351490688307	19.703948202915136	27.718459053637716	30.219241252758838
49	21.69890830371312	23.2788095915934	29.868590547652136	25.15369155704134
50	21.454946739991374	24.070028666540765	27.493805828581415	26.98121876488645
51	21.067370598896748	21.71102886667099	29.899314765382545	27.32228576904972
52	22.91801143842895	19.5291865974761	32.078479235784414	25.474322728310533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90.0
1	229.5
2	369.0
3	1670.5
4	2972.0
5	1989.0
6	1006.0
7	1014.5
8	1023.0
9	1078.0
10	1133.0
11	1095.5
12	1058.0
13	1007.0
14	890.5
15	825.0
16	792.0
17	759.0
18	812.0
19	865.0
20	954.0
21	1043.0
22	1310.5
23	1578.0
24	1880.5
25	2183.0
26	2653.5
27	3124.0
28	4469.5
29	5815.0
30	6105.0
31	6395.0
32	6668.5
33	6942.0
34	8907.5
35	10873.0
36	11390.0
37	11907.0
38	13565.0
39	18225.5
40	21228.0
41	25999.0
42	30770.0
43	39157.0
44	47544.0
45	51290.5
46	55037.0
47	56409.0
48	57781.0
49	64178.5
50	70576.0
51	72191.5
52	73807.0
53	67636.0
54	61465.0
55	52497.5
56	43530.0
57	37988.0
58	32446.0
59	31145.5
60	29845.0
61	29804.5
62	29764.0
63	27150.0
64	20635.5
65	16735.0
66	14089.0
67	11443.0
68	9953.5
69	8464.0
70	7298.5
71	6133.0
72	5528.0
73	4923.0
74	4089.5
75	3256.0
76	2814.5
77	2373.0
78	1672.5
79	972.0
80	660.0
81	348.0
82	338.5
83	329.0
84	199.0
85	69.0
86	41.0
87	13.0
88	10.0
89	5.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	709538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.193531352698095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3055902189551	17.964396228038883
2	10.597052381002852	5.339543428139586
3	3.7215396660146274	2.8127617876814734
4	1.8757085715850323	1.8902289082700827
5	1.1068634530133399	1.3942899553323624
6	0.7283354982950913	1.1009605926948223
7	0.6084835391103914	1.0730894384124838
8	0.516227146217836	1.0404467834682334
9	0.4712585693288	1.0685400787451496
>10	8.503651894750732	52.169765713892666
>50	0.4671485996209487	7.660950186246332
>100	0.0886068172150165	4.056320746678153
>500	0.006729631687216442	1.2578691608385788
>1k	0.002804013203006851	1.170836991561193
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	2006	0.2827191778312085	TruSeq Adapter, Index 22 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT	1943	0.27384016078067697	Illumina Single End Adapter 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1603	0.22592165606352302	No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	1590	0.22408947794198478	TruSeq Adapter, Index 22 (96% over 25bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1145	0.16137261147394502	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	997	0.14051396824412504	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	979	0.13797710622968748	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	950	0.1338899396508714	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	894	0.125997480050399	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	887	0.12501092260033994	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	761	0.107252888499277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28469229273132657	0.0
2	0.0	0.0	0.0	0.9634438183719547	0.0
3	0.0	0.0	0.0	1.5589017078718828	0.0
4	0.0	0.0	0.0	1.951128762659646	0.0
5	0.0	0.0	0.0	4.100273699224002	0.0
6	0.0	0.0	0.0	4.564942258201816	0.0
7	0.0	0.0	0.0	6.8028773652714865	0.0
8	0.0	0.0	0.0	9.154266579097948	0.0
9	0.0	0.0	0.0	11.535393453204762	0.0
10	0.0	0.0	0.0	12.476992070896838	0.0
11	0.0	0.0	0.0	13.409006987645482	0.0
12	0.0	0.0	0.0	14.196420769571185	0.0
13	0.0	0.0	0.0	14.4930926884818	0.0
14	0.0	0.0	0.0	14.706189097694557	0.0
15	0.0	0.0	0.0	15.457664001082394	0.0
16	0.0	0.0	0.0	16.27467450650987	0.0
17	0.0	0.0	0.0	17.432892952879197	0.0
18	0.0	0.0	0.0	17.90827270702908	0.0
19	0.0	0.0	0.0	18.579695520183556	0.0
20	0.0	0.0	0.0	19.27183603978927	0.0
21	0.0	0.0	0.0	19.921836462599607	0.0
22	0.0	0.0	0.0	20.587058057496566	0.0
23	1.4093677857986465E-4	0.0	0.0	21.078786477961717	0.0
24	1.4093677857986465E-4	0.0	0.0	21.508502715851723	0.0
25	1.4093677857986465E-4	0.0	0.0	21.903125695875346	0.0
26	1.4093677857986465E-4	0.0	0.0	22.30479551482796	0.0
27	1.4093677857986465E-4	0.0	0.0	22.69984130518732	0.0
28	1.4093677857986465E-4	0.0	0.0	23.102074871254253	0.0
29	1.4093677857986465E-4	0.0	0.0	23.627628118578567	0.0
30	2.818735571597293E-4	0.0	0.0	24.109913774878866	0.0
31	2.818735571597293E-4	0.0	0.0	24.578810437214074	0.0
32	2.818735571597293E-4	0.0	0.0	25.018674123161833	0.0
33	2.818735571597293E-4	0.0	0.0	25.419780195000126	0.0
34	2.818735571597293E-4	0.0	0.0	25.933353816145154	0.0
35	2.818735571597293E-4	0.0	0.0	26.346721387719896	0.0
36	2.818735571597293E-4	0.0	0.0	26.78968568279641	0.0
37	2.818735571597293E-4	0.0	0.0	27.22856281129411	0.0
38	2.818735571597293E-4	0.0	0.0	27.620226118967555	0.0
39	2.818735571597293E-4	0.0	0.0	28.06290854048691	0.0
40	2.818735571597293E-4	0.0	0.0	28.491779157705437	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGT	35	1.01883415E-7	46.000004	20
ATTACGG	35	1.01883415E-7	46.000004	1
TTCACGG	55	1.8189894E-12	46.000004	1
CCGTAGG	35	1.01883415E-7	46.000004	1
AACCGCA	20	6.309814E-4	46.0	22
AATCCGC	20	6.309814E-4	46.0	39
TACCCGT	30	1.8603878E-6	46.0	35
CCGATTT	20	6.309814E-4	46.0	11
CAACGAC	25	3.4158256E-5	46.0	11
CTATCGG	20	6.309814E-4	46.0	1
GTCCTAG	25	3.4158256E-5	46.0	42
ATAGCGG	90	0.0	46.0	1
CACGATT	20	6.309814E-4	46.0	38
TACGGAC	20	6.309814E-4	46.0	16
GCCGATG	30	1.8603878E-6	46.0	8
GGTATAC	20	6.309814E-4	46.0	7
AACGACA	20	6.309814E-4	46.0	24
GCGATTG	60	0.0	46.0	8
GCCTATA	20	6.309814E-4	46.0	12
AGTTACC	20	6.309814E-4	46.0	21
>>END_MODULE