FastQCFastQC Report
Thu 26 May 2016
SRR1544917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544917_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences452215
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT18130.40091549373638646TruSeq Adapter, Index 22 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT15510.3429784505157945Illumina Single End Adapter 2 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA14030.31025065510874256No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT13810.3053857125482348TruSeq Adapter, Index 22 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.2191435489755979No Hit
TGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTTG8560.1892904923543005TruSeq Adapter, Index 22 (96% over 27bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6180.13666065919971693No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC5130.1134416151609301No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT5070.11211481264442798No Hit
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC4830.10680760257841956TruSeq Adapter, Index 22 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC4780.10570193381466779Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA4750.10503853255641675No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT4730.10459626505091606No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTA206.307373E-446.00000443
CACGAGT206.307373E-446.00000437
CACGACG206.307373E-446.00000425
TACATCG206.307373E-446.0000041
AGCGACA206.307373E-446.00000431
ACGTAGG750.046.0000041
AATACGG206.307373E-446.0000041
AGTCGAG206.307373E-446.00000429
CAACCGG453.092282E-1046.01
ACTAGTG600.046.011
CCGTAGG253.4138462E-546.01
CGGGACC253.4138462E-546.05
ATGACGG253.4138462E-546.01
GCGATAG600.046.08
TAGGCGG253.4138462E-546.01
GGCGATA850.046.07
GCCACGT253.4138462E-546.020
ATCGAGG453.092282E-1046.01
TAAGGGA1200.044.0833323
CGGGATT1000.043.6999975
Produced by FastQC (version 0.11.5)