##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544917_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	452215
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87815972490961	34.0	33.0	34.0	31.0	34.0
2	33.253251218999814	34.0	33.0	34.0	31.0	34.0
3	33.383631679621416	34.0	34.0	34.0	31.0	34.0
4	36.61020532268943	37.0	37.0	37.0	35.0	37.0
5	36.62122884026403	37.0	37.0	37.0	35.0	37.0
6	36.404814081797376	37.0	37.0	37.0	35.0	37.0
7	36.18612827968997	37.0	37.0	37.0	35.0	37.0
8	36.34022533529405	37.0	37.0	37.0	35.0	37.0
9	38.2390190506728	39.0	39.0	39.0	37.0	39.0
10	38.136822086839224	39.0	39.0	39.0	35.0	39.0
11	37.62909014517431	39.0	37.0	39.0	35.0	39.0
12	37.60483619517265	39.0	37.0	39.0	35.0	39.0
13	37.60163860110788	39.0	37.0	39.0	35.0	39.0
14	38.876605154627775	41.0	37.0	41.0	35.0	41.0
15	38.901858629191864	41.0	37.0	41.0	35.0	41.0
16	38.927421691009805	41.0	37.0	41.0	35.0	41.0
17	39.01898212133609	41.0	37.0	41.0	35.0	41.0
18	39.0036022688323	41.0	37.0	41.0	35.0	41.0
19	38.96834470329379	41.0	37.0	41.0	35.0	41.0
20	38.92115697179439	41.0	36.0	41.0	35.0	41.0
21	38.855730128368144	41.0	35.0	41.0	35.0	41.0
22	38.82483774310892	41.0	35.0	41.0	35.0	41.0
23	38.78879073007308	41.0	35.0	41.0	35.0	41.0
24	38.738067069867206	41.0	35.0	41.0	35.0	41.0
25	38.69000364870692	41.0	35.0	41.0	35.0	41.0
26	38.61239454684166	40.0	35.0	41.0	35.0	41.0
27	38.70028636820981	41.0	36.0	41.0	35.0	41.0
28	38.70241146357374	41.0	36.0	41.0	35.0	41.0
29	38.719000917705074	41.0	36.0	41.0	35.0	41.0
30	38.67014804904747	41.0	36.0	41.0	35.0	41.0
31	38.57720774410402	41.0	35.0	41.0	35.0	41.0
32	38.53816658005595	41.0	35.0	41.0	35.0	41.0
33	38.45240427672678	40.0	35.0	41.0	35.0	41.0
34	38.32571896111363	40.0	35.0	41.0	35.0	41.0
35	38.27922116692282	40.0	35.0	41.0	35.0	41.0
36	38.21792289066042	40.0	35.0	41.0	35.0	41.0
37	38.15278573245027	40.0	35.0	41.0	35.0	41.0
38	38.07663611335316	40.0	35.0	41.0	35.0	41.0
39	38.02464093406897	40.0	35.0	41.0	35.0	41.0
40	37.96853266698363	40.0	35.0	41.0	35.0	41.0
41	37.91954490673684	40.0	35.0	41.0	35.0	41.0
42	37.85124995853742	40.0	35.0	41.0	35.0	41.0
43	37.78371792178499	40.0	35.0	41.0	35.0	41.0
44	37.64013356478666	40.0	35.0	41.0	35.0	41.0
45	37.62487091317183	40.0	35.0	41.0	35.0	41.0
46	37.55982884247537	39.0	35.0	41.0	35.0	41.0
47	37.47855997700209	39.0	35.0	41.0	35.0	41.0
48	37.41322379841447	39.0	35.0	41.0	35.0	41.0
49	37.344216799531196	39.0	35.0	41.0	35.0	41.0
50	37.270044116183676	39.0	35.0	41.0	35.0	41.0
51	37.193323972004464	39.0	35.0	41.0	34.0	41.0
52	36.72438110190949	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	5.0
14	4.0
15	3.0
16	10.0
17	19.0
18	34.0
19	75.0
20	133.0
21	167.0
22	257.0
23	413.0
24	677.0
25	1167.0
26	1759.0
27	1995.0
28	1971.0
29	2033.0
30	2340.0
31	2798.0
32	3957.0
33	5804.0
34	20017.0
35	77909.0
36	17612.0
37	28609.0
38	50672.0
39	229673.0
40	2100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.174043320102165	22.30598277368066	22.597658193558374	21.9223157126588
2	27.75294937142731	24.38375551452296	25.427949095010117	22.435346019039613
3	27.95528675519388	25.074135090609555	24.52926152383269	22.441316630363875
4	25.862698052917306	27.35645655274593	22.0180666275997	24.76277876673706
5	24.281149453246798	35.92295700054178	20.167619384584768	19.62827416162666
6	85.93257631878642	3.3499552204150675	7.45198633393408	3.2654821268644336
7	84.85897194918346	3.320986698804772	6.245038311422664	5.5750030405891
8	73.11875988191457	5.986532954457504	12.577866722687217	8.316840440940702
9	48.746503322534636	19.967493338345697	15.509879150404121	15.776124188715546
10	37.46890306601948	21.149674380549076	26.15813274659178	15.223289806839668
11	36.62549893302965	16.54168924073726	26.01019426600179	20.822617560231304
12	21.82037305264089	28.005705250820963	30.202447950642945	19.971473745895203
13	15.863029753546432	30.428446645953805	30.214610307044214	23.493913293455545
14	15.384275178841921	22.920071205068385	40.626693055294496	21.068960560795198
15	17.00894486029875	17.61794721537322	42.5846113021461	22.788496622181928
16	16.963612440984928	21.44245546919054	29.761286113905992	31.832645975918535
17	19.618102009000143	23.18742191214356	32.11083223687848	25.08364384197782
18	22.8331656402375	27.716904569729	26.844089647623363	22.605840142410134
19	25.58119478566611	23.075970500757386	25.95690103158896	25.38593368198755
20	21.357982375639907	29.720376369647184	26.350076843979082	22.57156441073383
21	20.873036055858385	24.583439293256525	24.751058677841293	29.792465973043797
22	20.00420154130226	29.185674955496832	23.573079176940173	27.23704432626074
23	19.41996616653583	22.526010857667263	34.51190252424179	23.542120451555125
24	20.086021029819886	22.60208086861338	31.473303627699213	25.83859447386752
25	18.486118328671097	27.183529958095153	29.308625322026028	25.02172639120772
26	19.503775858828213	26.408235020952425	27.789657574383863	26.298331545835495
27	22.468294948199418	25.447187731499398	32.24063774974294	19.84387957055825
28	20.431431951615934	25.05954026292803	28.305341485797687	26.203686299658347
29	21.705162367457955	25.573455104319848	27.593511935694302	25.12787059252789
30	28.410380018354104	23.428678836394194	21.873887420806476	26.28705372444523
31	27.449553862653826	24.839733312694186	24.605994936037064	23.10471788861493
32	26.086927678206163	23.859447386751878	24.781796269473592	25.271828665568368
33	23.03196488396006	24.59405371338854	27.03360127373042	25.340380128920977
34	22.735424521521843	23.292681578452726	28.103667503289365	25.868226396736066
35	29.721482038410933	22.889997014694337	23.153588447972755	24.234932498921975
36	24.27053503311478	24.357661731698418	25.61922979113917	25.752573444047634
37	26.470152471722518	24.5038311422664	26.97389516048782	22.05212122552326
38	23.353272226706324	23.506517917362316	25.04361863273001	28.096591223201354
39	28.272834824143384	19.69173954866601	26.762270159105732	25.273155468084873
40	19.733976095441328	18.74705615691651	30.43751312981657	31.081454617825592
41	22.804639386132703	23.884877768318166	25.355196090355253	27.95528675519388
42	23.951439027896022	21.066749223267696	27.065886801631965	27.915924947204317
43	24.52815585506894	25.739968820140863	25.93898919761618	23.79288612717402
44	20.21472087392059	25.880388753137336	28.386718706809816	25.51817166613226
45	23.850823170394612	24.59361144588304	26.792786616985282	24.76277876673706
46	24.759240626693053	21.296064924869807	28.31993631347921	25.624758134957933
47	24.326039605055115	19.257653992017072	30.202005683137443	26.21430071979037
48	22.143449465409155	18.673860884756145	27.240803600057497	31.941886049777207
49	21.470760589542586	21.797154008602103	30.420928098360296	26.31115730349502
50	21.87786782835598	22.73586678902734	28.104773172053115	27.281492210563556
51	21.135079552867552	20.474995300907754	30.12593567219132	28.26398947403337
52	22.526895392678263	18.718308769058968	32.87020554382318	25.88459029443959
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	48.0
1	158.0
2	268.0
3	1046.0
4	1824.0
5	1250.0
6	676.0
7	665.0
8	654.0
9	686.0
10	718.0
11	725.0
12	732.0
13	654.0
14	568.5
15	561.0
16	525.5
17	490.0
18	518.5
19	547.0
20	564.5
21	582.0
22	733.5
23	885.0
24	1074.5
25	1264.0
26	1489.5
27	1715.0
28	2313.5
29	2912.0
30	3156.0
31	3400.0
32	3592.5
33	3785.0
34	4956.5
35	6128.0
36	6498.5
37	6869.0
38	7967.0
39	10521.0
40	11977.0
41	14868.0
42	17759.0
43	22609.0
44	27459.0
45	30079.0
46	32699.0
47	34300.0
48	35901.0
49	42098.5
50	48296.0
51	50185.5
52	52075.0
53	47497.0
54	42919.0
55	36246.5
56	29574.0
57	25381.0
58	21188.0
59	19998.0
60	18808.0
61	18710.0
62	18612.0
63	17399.5
64	13346.5
65	10506.0
66	8947.0
67	7388.0
68	6331.0
69	5274.0
70	4611.5
71	3949.0
72	3578.5
73	3208.0
74	2656.5
75	2105.0
76	1760.0
77	1415.0
78	1058.0
79	701.0
80	475.0
81	249.0
82	221.0
83	193.0
84	125.0
85	57.0
86	35.0
87	13.0
88	8.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	452215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.28998990014198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.52361939649981	21.66449709141578
2	9.68350043085049	5.866262604969637
3	3.412076619211466	3.1005529900327766
4	1.7528290952004693	2.1237270236118886
5	1.2237991872978318	1.8534432511526644
6	0.9537676455572153	1.7333767410606147
7	0.8602175245076319	1.823918609048291
8	0.7975720728354081	1.9326760024655851
9	0.7088382717775177	1.9323633683677595
>10	8.837758596144027	48.687477009835526
>50	0.1874446934233567	3.7765562242603608
>100	0.05198661419163409	3.3686934748819954
>500	0.003661029168424936	0.7729209796539439
>1k	0.0029288233347399485	1.36353462924317
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	1813	0.40091549373638646	TruSeq Adapter, Index 22 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT	1551	0.3429784505157945	Illumina Single End Adapter 2 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1403	0.31025065510874256	No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	1381	0.3053857125482348	TruSeq Adapter, Index 22 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	991	0.2191435489755979	No Hit
TGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTTG	856	0.1892904923543005	TruSeq Adapter, Index 22 (96% over 27bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	618	0.13666065919971693	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	513	0.1134416151609301	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	507	0.11211481264442798	No Hit
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	483	0.10680760257841956	TruSeq Adapter, Index 22 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	478	0.10570193381466779	Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	475	0.10503853255641675	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	473	0.10459626505091606	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35226606813130923	0.0
2	0.0	0.0	0.0	1.261789193193503	0.0
3	2.2113375275035105E-4	0.0	0.0	1.9725130745331314	0.0
4	2.2113375275035105E-4	0.0	0.0	2.458122795572902	0.0
5	2.2113375275035105E-4	0.0	0.0	5.000939818449189	0.0
6	2.2113375275035105E-4	0.0	0.0	5.572570569308846	0.0
7	2.2113375275035105E-4	0.0	0.0	8.315513638424202	0.0
8	2.2113375275035105E-4	0.0	0.0	11.090078834182856	0.0
9	2.2113375275035105E-4	0.0	0.0	14.096613336576628	0.0
10	2.2113375275035105E-4	0.0	0.0	15.248941322158707	0.0
11	2.2113375275035105E-4	0.0	0.0	16.373406454894244	0.0
12	2.2113375275035105E-4	0.0	0.0	17.34086662317703	0.0
13	2.2113375275035105E-4	0.0	0.0	17.702641442676605	0.0
14	2.2113375275035105E-4	0.0	0.0	17.967338544718775	0.0
15	2.2113375275035105E-4	0.0	0.0	18.855853963269684	0.0
16	2.2113375275035105E-4	0.0	0.0	19.75874307574937	0.0
17	2.2113375275035105E-4	0.0	0.0	20.98183386221156	0.0
18	2.2113375275035105E-4	0.0	0.0	21.5125548688124	0.0
19	2.2113375275035105E-4	0.0	0.0	22.226816890196034	0.0
20	2.2113375275035105E-4	0.0	0.0	22.986411330893493	0.0
21	4.422675055007021E-4	0.0	0.0	23.695808409716616	0.0
22	4.422675055007021E-4	0.0	0.0	24.424665258781776	0.0
23	4.422675055007021E-4	0.0	0.0	24.97130789558064	0.0
24	4.422675055007021E-4	0.0	0.0	25.421536216180357	0.0
25	4.422675055007021E-4	0.0	0.0	25.86601505920856	0.0
26	4.422675055007021E-4	0.0	0.0	26.309388233473015	0.0
27	4.422675055007021E-4	0.0	0.0	26.724677421138175	0.0
28	4.422675055007021E-4	0.0	0.0	27.132226927457072	0.0
29	4.422675055007021E-4	0.0	0.0	27.64724743761264	0.0
30	4.422675055007021E-4	0.0	0.0	28.13197262364141	0.0
31	4.422675055007021E-4	0.0	0.0	28.627533363554946	0.0
32	4.422675055007021E-4	0.0	0.0	29.053436971352124	0.0
33	4.422675055007021E-4	0.0	0.0	29.46076534391827	0.0
34	4.422675055007021E-4	0.0	0.0	29.9848523379366	0.0
35	4.422675055007021E-4	0.0	0.0	30.421591499618543	0.0
36	4.422675055007021E-4	0.0	0.0	30.876463629026016	0.0
37	4.422675055007021E-4	0.0	0.0	31.316298663246464	0.0
38	4.422675055007021E-4	0.0	0.0	31.730924449653372	0.0
39	4.422675055007021E-4	0.0	0.0	32.184912044049845	0.0
40	4.422675055007021E-4	0.0	0.0	32.63469809714406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCTA	20	6.307373E-4	46.000004	43
CACGAGT	20	6.307373E-4	46.000004	37
CACGACG	20	6.307373E-4	46.000004	25
TACATCG	20	6.307373E-4	46.000004	1
AGCGACA	20	6.307373E-4	46.000004	31
ACGTAGG	75	0.0	46.000004	1
AATACGG	20	6.307373E-4	46.000004	1
AGTCGAG	20	6.307373E-4	46.000004	29
CAACCGG	45	3.092282E-10	46.0	1
ACTAGTG	60	0.0	46.0	11
CCGTAGG	25	3.4138462E-5	46.0	1
CGGGACC	25	3.4138462E-5	46.0	5
ATGACGG	25	3.4138462E-5	46.0	1
GCGATAG	60	0.0	46.0	8
TAGGCGG	25	3.4138462E-5	46.0	1
GGCGATA	85	0.0	46.0	7
GCCACGT	25	3.4138462E-5	46.0	20
ATCGAGG	45	3.092282E-10	46.0	1
TAAGGGA	120	0.0	44.083332	3
CGGGATT	100	0.0	43.699997	5
>>END_MODULE