FastQCFastQC Report
Thu 26 May 2016
SRR1544916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544916_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences875244
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT33170.37898003299651295No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTT31470.35955687785349Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT29470.3367061070969924No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19630.2242803149750241No Hit
ACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC13970.15961263373413584No Hit
TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT13730.15687054124335614No Hit
GCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC9630.11002646119253603No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC9310.10637033787149641No Hit
ACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT9010.10294272225802176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG1550.046.0000041
GCGAACG206.310626E-446.08
GCAAACG206.310626E-446.033
ACGGGAC1800.046.04
CGGATCC301.8608898E-646.09
ACTACGG301.8608898E-646.01
GCTAGTT206.310626E-446.08
ACGTAGG1050.046.01
CGGTCAT206.310626E-446.015
CCAAATC206.310626E-446.012
CCACGAT206.310626E-446.043
GCCACGA206.310626E-446.042
TATCGGC206.310626E-446.043
GTGCGGC253.4164827E-546.09
TTCGAAC206.310626E-446.010
GCGTATT206.310626E-446.042
TACACGG850.046.01
TCAACGG1100.046.01
TACGTTC206.310626E-446.016
TACGTAG206.310626E-446.018
Produced by FastQC (version 0.11.5)