##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544916_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	875244
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.865116470378545	34.0	33.0	34.0	31.0	34.0
2	33.178471374839475	34.0	33.0	34.0	31.0	34.0
3	33.23990681455685	34.0	33.0	34.0	31.0	34.0
4	36.54209454734908	37.0	37.0	37.0	35.0	37.0
5	36.535159338424485	37.0	37.0	37.0	35.0	37.0
6	36.645640529955074	37.0	37.0	37.0	35.0	37.0
7	36.50188061843326	37.0	37.0	37.0	35.0	37.0
8	36.52408813999296	37.0	37.0	37.0	35.0	37.0
9	38.29649446325825	39.0	39.0	39.0	37.0	39.0
10	38.17888268871309	39.0	39.0	39.0	35.0	39.0
11	37.64547371932855	39.0	37.0	39.0	35.0	39.0
12	37.59153447495784	39.0	37.0	39.0	35.0	39.0
13	37.57934930145194	39.0	37.0	39.0	35.0	39.0
14	38.942552019779626	41.0	37.0	41.0	35.0	41.0
15	38.97022316062721	41.0	37.0	41.0	35.0	41.0
16	38.968665880600156	41.0	37.0	41.0	35.0	41.0
17	39.018361736841385	41.0	37.0	41.0	35.0	41.0
18	39.00218453368432	41.0	37.0	41.0	35.0	41.0
19	38.935136944669146	41.0	37.0	41.0	35.0	41.0
20	38.86465716988634	41.0	36.0	41.0	35.0	41.0
21	38.8382074027357	41.0	36.0	41.0	35.0	41.0
22	38.80435741347556	41.0	36.0	41.0	35.0	41.0
23	38.80340910648916	41.0	36.0	41.0	35.0	41.0
24	38.76078327871999	41.0	36.0	41.0	35.0	41.0
25	38.72466763553935	41.0	36.0	41.0	35.0	41.0
26	38.66274547440485	41.0	35.0	41.0	35.0	41.0
27	38.642411716047185	41.0	36.0	41.0	35.0	41.0
28	38.650460900046156	41.0	36.0	41.0	35.0	41.0
29	38.65928701025086	41.0	36.0	41.0	35.0	41.0
30	38.601863023339774	41.0	36.0	41.0	35.0	41.0
31	38.51607665976574	40.0	35.0	41.0	35.0	41.0
32	38.466451640913846	40.0	36.0	41.0	35.0	41.0
33	38.346855276928494	40.0	36.0	41.0	35.0	41.0
34	38.24716993204181	40.0	35.0	41.0	35.0	41.0
35	38.180428543354765	40.0	35.0	41.0	35.0	41.0
36	38.15688539424435	40.0	35.0	41.0	35.0	41.0
37	38.066617994524954	40.0	35.0	41.0	35.0	41.0
38	38.04295145125245	40.0	35.0	41.0	35.0	41.0
39	37.96852420582146	40.0	35.0	41.0	35.0	41.0
40	37.88825973100073	40.0	35.0	41.0	35.0	41.0
41	37.83752302215154	40.0	35.0	41.0	35.0	41.0
42	37.755396209514146	40.0	35.0	41.0	35.0	41.0
43	37.67091348241176	40.0	35.0	41.0	34.0	41.0
44	37.608246386150604	40.0	35.0	41.0	34.0	41.0
45	37.567564016434275	40.0	35.0	41.0	34.0	41.0
46	37.60402127863773	40.0	35.0	41.0	34.0	41.0
47	37.532007074598624	40.0	35.0	41.0	34.0	41.0
48	37.53884059759336	40.0	35.0	41.0	34.0	41.0
49	37.47928920392485	39.0	35.0	41.0	34.0	41.0
50	37.40646379752389	39.0	35.0	41.0	34.0	41.0
51	37.321462357925334	39.0	35.0	41.0	34.0	41.0
52	37.099536814876764	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	9.0
14	8.0
15	12.0
16	14.0
17	34.0
18	67.0
19	135.0
20	226.0
21	356.0
22	572.0
23	930.0
24	1627.0
25	2809.0
26	3985.0
27	4711.0
28	4854.0
29	5322.0
30	6178.0
31	7446.0
32	9529.0
33	13270.0
34	35413.0
35	139823.0
36	28950.0
37	44298.0
38	87066.0
39	473882.0
40	3712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.340517615659174	23.45083199656324	26.66753499595541	18.541115391822167
2	26.04290917732655	24.033298143146368	27.61252862059037	22.311264058936707
3	25.419425897235513	25.50637307996399	26.554080919149403	22.520120103651095
4	25.237191000452448	28.07537098226323	23.672827234462616	23.014610782821705
5	23.187362609740827	35.181160910557516	21.498804904689436	20.132671575012225
6	87.65498535265594	2.169909191037014	7.5913688068698555	2.583736649437186
7	88.63916804913829	1.8052108897633117	5.122114518922723	4.433506542175667
8	77.2305779873955	3.983689119834012	11.46503146551133	7.320701427259141
9	40.94778141866725	17.213599864723435	16.652841950358983	25.185776766250324
10	34.081467567901065	18.776249822906525	30.017229481150398	17.12505312804201
11	33.90608790234494	18.72860596587923	26.689814497443	20.675491634332825
12	21.58072491785148	28.475830739770853	30.55467960934322	19.388764733034446
13	16.390515102074392	31.480021571127594	31.497388156902534	20.63207516989548
14	15.700421825228164	24.36109244964833	40.476827033375834	19.461658691747672
15	18.093811554263723	20.10764998103386	41.40822444941068	20.390314015291736
16	18.079301314833348	22.956227063538854	28.765007243694328	30.199464377933467
17	19.5547755825804	23.3265238036479	33.14115835127119	23.977542262500513
18	22.023572855112402	28.34546709260503	29.5938046990325	20.037155353250068
19	24.25803547353652	24.266490258716427	26.768192641137784	24.707281626609266
20	20.461379912344444	30.7655922234257	27.80858823368112	20.96443963054874
21	20.1243310436861	24.12070234128997	28.606994163913146	27.147972451110775
22	17.53373916302197	28.06417410459255	26.68752942036735	27.714557312018133
23	17.972359707692938	23.30550109455192	36.069941639131486	22.65219755862365
24	20.37443272961597	23.202558372293897	32.11275941337502	24.31024948471512
25	17.0044010584477	29.063781071335537	30.068072446083605	23.863745424133157
26	17.976358592575327	27.440462316793944	29.094058342587896	25.489120748042833
27	20.241098482251807	27.035432405135023	32.566347212891486	20.157121899721677
28	17.857991600056668	25.546247674934076	29.669326496382727	26.926434228626533
29	19.22698127607844	29.852703931703616	27.814872195639158	23.105442596578783
30	26.61977688507433	26.546768672507326	23.56679965815247	23.26665478426587
31	24.850898720813852	27.50318768252053	23.8236423214555	23.822271275210113
32	23.941438044705247	29.849504823797705	23.689622550968643	22.5194345805284
33	20.014076074786004	29.10845432816449	25.43176531344402	25.445704283605487
34	21.92474327159055	26.046793808355158	27.37362381233119	24.654839107723102
35	24.207192508603313	28.003276800526482	23.94258058324307	23.84695010762713
36	25.245417277924787	29.894063826772875	24.023243804013507	20.837275091288827
37	21.745250467298263	29.483778237839964	24.599083227077248	24.17188806778453
38	22.080585528149864	27.343917810347744	23.9906814556855	26.58481520581689
39	25.618341856670824	24.847699612907945	25.324709452449827	24.2092490779714
40	20.19493992532368	23.30767191777379	28.75358185831608	27.74380629858645
41	22.189583704658357	26.171673270539415	23.633066893346314	28.005676131455914
42	22.178044065426327	22.643514265736183	28.074913966848104	27.10352770198939
43	23.109098719899823	26.601382014615353	26.698726298038032	23.59079296744679
44	19.23098016096083	30.33599773320354	26.13842539908871	24.29459670674692
45	22.062647673106014	27.325180178327415	25.889694759404236	24.722477389162336
46	24.47431801874677	23.50110369222754	26.124486428927245	25.90009186009844
47	21.92839939491159	21.914231917042564	30.263903551466793	25.893465136579056
48	22.70623963146277	21.061098390848723	27.570597456252198	28.66206452143631
49	21.20323018495414	24.20113705435284	29.599288884014058	24.99634387667896
50	20.487658298714415	24.795942617144476	28.062574550639592	26.653824533501513
51	19.93215606162396	21.94668001151679	29.350329736622015	28.770834190237238
52	21.45630247108235	21.004999748641524	33.42610746260471	24.112590317671415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95.0
1	239.5
2	384.0
3	2230.5
4	4077.0
5	2730.5
6	1384.0
7	1416.5
8	1449.0
9	1497.5
10	1546.0
11	1465.0
12	1384.0
13	1347.0
14	1217.0
15	1124.0
16	1136.5
17	1149.0
18	1077.0
19	1005.0
20	1245.0
21	1485.0
22	1745.5
23	2006.0
24	2341.5
25	2677.0
26	3306.5
27	3936.0
28	4628.5
29	5321.0
30	6736.5
31	8152.0
32	9304.0
33	10456.0
34	12250.5
35	14045.0
36	15217.5
37	16390.0
38	19315.0
39	25281.0
40	28322.0
41	34417.0
42	40512.0
43	50878.5
44	61245.0
45	66513.5
46	71782.0
47	78068.0
48	84354.0
49	94965.5
50	105577.0
51	106200.5
52	106824.0
53	95379.0
54	83934.0
55	70411.0
56	56888.0
57	48457.0
58	40026.0
59	35890.0
60	31754.0
61	27338.0
62	22922.0
63	19601.0
64	12641.5
65	9003.0
66	7390.5
67	5778.0
68	4736.5
69	3695.0
70	2820.5
71	1946.0
72	1547.5
73	1149.0
74	940.5
75	732.0
76	684.0
77	636.0
78	391.0
79	146.0
80	106.5
81	67.0
82	56.0
83	45.0
84	24.0
85	3.0
86	5.5
87	8.0
88	4.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	875244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.97078996368813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.6274307902065	19.47534242344098
2	12.921356833657471	7.22841116080199
3	5.046024973158974	4.234239140272641
4	2.3629068378367677	2.6436948345957885
5	1.3492633950025927	1.8869981513655147
6	0.8461121924866459	1.419985585305578
7	0.5755491700074225	1.126899546363685
8	0.4700844511296706	1.0518906758194906
9	0.3683539760621158	0.9272836527050563
>10	5.5814127903745145	39.28218299602598
>50	0.7304453407762626	12.770144615828757
>100	0.1136975381949465	5.3698645950932
>500	0.004907807404098409	0.9650450080783588
>1k	0.0024539037020492047	1.6180176143030234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	3317	0.37898003299651295	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTT	3147	0.35955687785349	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	2947	0.3367061070969924	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1963	0.2242803149750241	No Hit
ACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1397	0.15961263373413584	No Hit
TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	1373	0.15687054124335614	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	963	0.11002646119253603	No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	931	0.10637033787149641	No Hit
ACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	901	0.10294272225802176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3654980782501794	0.0
2	0.0	0.0	0.0	1.3589353368889132	0.0
3	0.0	0.0	0.0	2.198015639067506	0.0
4	0.0	0.0	0.0	2.694334379898634	0.0
5	0.0	0.0	0.0	5.695554611057031	0.0
6	0.0	0.0	0.0	6.198043059992414	0.0
7	0.0	0.0	0.0	8.524822792272783	0.0
8	0.0	0.0	0.0	10.947233000169096	0.0
9	0.0	0.0	0.0	13.134851538542396	0.0
10	0.0	0.0	0.0	14.380675560186646	0.0
11	0.0	0.0	0.0	15.524699398110698	0.0
12	0.0	0.0	0.0	16.386401963338223	0.0
13	0.0	0.0	0.0	16.73247688644538	0.0
14	0.0	0.0	0.0	16.996403288682927	0.0
15	0.0	0.0	0.0	18.062163236765976	0.0
16	0.0	0.0	0.0	19.306044942895923	0.0
17	0.0	0.0	0.0	20.759125455301607	0.0
18	0.0	0.0	0.0	21.516057236610592	0.0
19	0.0	0.0	0.0	22.33160124490999	0.0
20	0.0	0.0	0.0	23.384793269076965	0.0
21	0.0	0.0	0.0	24.336299363377528	0.0
22	0.0	0.0	0.0	25.361727701075356	0.0
23	0.0	0.0	0.0	26.140253460749232	0.0
24	1.1425385378248809E-4	0.0	0.0	26.81526522889617	0.0
25	1.1425385378248809E-4	0.0	0.0	27.473595934390868	0.0
26	1.1425385378248809E-4	0.0	0.0	28.072857397480018	0.0
27	1.1425385378248809E-4	0.0	0.0	28.680002376480157	0.0
28	1.1425385378248809E-4	0.0	0.0	29.304513941255237	0.0
29	1.1425385378248809E-4	0.0	0.0	30.077212754386206	0.0
30	1.1425385378248809E-4	0.0	0.0	30.7473116068205	0.0
31	1.1425385378248809E-4	0.0	0.0	31.307498251916037	0.0
32	1.1425385378248809E-4	0.0	0.0	31.909958822911097	0.0
33	1.1425385378248809E-4	0.0	0.0	32.49379601573961	0.0
34	1.1425385378248809E-4	0.0	0.0	33.20651155563477	0.0
35	1.1425385378248809E-4	0.0	0.0	33.77606701674048	0.0
36	1.1425385378248809E-4	0.0	0.0	34.34607949326131	0.0
37	1.1425385378248809E-4	0.0	0.0	34.9411135637605	0.0
38	1.1425385378248809E-4	0.0	0.0	35.52894964147141	0.0
39	1.1425385378248809E-4	0.0	0.0	36.16911398421469	0.0
40	1.1425385378248809E-4	0.0	0.0	36.785742033078776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	155	0.0	46.000004	1
GCGAACG	20	6.310626E-4	46.0	8
GCAAACG	20	6.310626E-4	46.0	33
ACGGGAC	180	0.0	46.0	4
CGGATCC	30	1.8608898E-6	46.0	9
ACTACGG	30	1.8608898E-6	46.0	1
GCTAGTT	20	6.310626E-4	46.0	8
ACGTAGG	105	0.0	46.0	1
CGGTCAT	20	6.310626E-4	46.0	15
CCAAATC	20	6.310626E-4	46.0	12
CCACGAT	20	6.310626E-4	46.0	43
GCCACGA	20	6.310626E-4	46.0	42
TATCGGC	20	6.310626E-4	46.0	43
GTGCGGC	25	3.4164827E-5	46.0	9
TTCGAAC	20	6.310626E-4	46.0	10
GCGTATT	20	6.310626E-4	46.0	42
TACACGG	85	0.0	46.0	1
TCAACGG	110	0.0	46.0	1
TACGTTC	20	6.310626E-4	46.0	16
TACGTAG	20	6.310626E-4	46.0	18
>>END_MODULE