FastQCFastQC Report
Thu 26 May 2016
SRR1544915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544915_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences574677
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT30750.535083185859187No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTT26150.45503822147049555Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT22520.39187230392028916No Hit
TGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTTG15180.2641483824826816Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA13900.24187500108756743No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.22168974919824527No Hit
ACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC10110.17592491086297174No Hit
TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT9970.1734887597728811No Hit
GCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC7180.12493974876321828No Hit
ACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT6860.11937140341443976No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC6850.11919739262229044No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC6660.11589118757145318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG453.092282E-1046.01
CGTTGGA206.308808E-446.027
TAACGTT206.308808E-446.046
CACGACG206.308808E-446.025
ATATCGG405.6024874E-946.01
CTACGAA405.6024874E-946.011
CTTGCAC206.308808E-446.023
ACGGATC206.308808E-446.04
GCGATGC650.046.08
GGGTTCG453.092282E-1046.06
ACGTAGG1050.046.01
CCAACGG253.415008E-546.01
ACGAGTC405.6024874E-946.039
CGATCTA253.415008E-546.09
TCTGGTA206.308808E-446.021
CGATACG206.308808E-446.09
TCAACGG405.6024874E-946.01
CTAGCGG1850.046.01
ACCGGGT351.0183794E-745.9999963
CTTGCGG1150.044.0000041
Produced by FastQC (version 0.11.5)