##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544915_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	574677
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88268192393292	34.0	33.0	34.0	31.0	34.0
2	33.239837334711495	34.0	33.0	34.0	31.0	34.0
3	33.36971028943215	34.0	34.0	34.0	31.0	34.0
4	36.60576114930648	37.0	37.0	37.0	35.0	37.0
5	36.60580639211244	37.0	37.0	37.0	35.0	37.0
6	36.400435375001955	37.0	37.0	37.0	35.0	37.0
7	36.279957262949445	37.0	37.0	37.0	35.0	37.0
8	36.401373293171645	37.0	37.0	37.0	35.0	37.0
9	38.267687761995	39.0	39.0	39.0	37.0	39.0
10	38.22638630047835	39.0	39.0	39.0	35.0	39.0
11	37.759250848737636	39.0	37.0	39.0	35.0	39.0
12	37.75221385230312	39.0	37.0	39.0	35.0	39.0
13	37.74432768320291	39.0	37.0	39.0	35.0	39.0
14	39.05731393461023	41.0	38.0	41.0	35.0	41.0
15	39.05080767109176	41.0	38.0	41.0	35.0	41.0
16	39.08232450576585	41.0	38.0	41.0	35.0	41.0
17	39.15972450611387	41.0	38.0	41.0	35.0	41.0
18	39.16571917790341	41.0	38.0	41.0	35.0	41.0
19	39.12785964985549	41.0	38.0	41.0	35.0	41.0
20	39.07284787802539	41.0	38.0	41.0	35.0	41.0
21	39.056939811407105	41.0	38.0	41.0	35.0	41.0
22	39.032771452485484	41.0	38.0	41.0	35.0	41.0
23	38.999203030571955	41.0	37.0	41.0	35.0	41.0
24	38.98019931196133	41.0	37.0	41.0	35.0	41.0
25	38.933781933155494	41.0	37.0	41.0	35.0	41.0
26	38.85774965763377	41.0	37.0	41.0	35.0	41.0
27	38.94063273804241	41.0	37.0	41.0	35.0	41.0
28	38.94189257617757	41.0	37.0	41.0	35.0	41.0
29	38.9554636778573	41.0	37.0	41.0	35.0	41.0
30	38.90695468932983	41.0	37.0	41.0	35.0	41.0
31	38.84462576368986	41.0	37.0	41.0	35.0	41.0
32	38.79417133450617	41.0	37.0	41.0	35.0	41.0
33	38.70772277296638	41.0	37.0	41.0	35.0	41.0
34	38.606032606142236	41.0	37.0	41.0	35.0	41.0
35	38.529861121986784	41.0	36.0	41.0	35.0	41.0
36	38.52854908061398	41.0	36.0	41.0	35.0	41.0
37	38.44721121603962	40.0	36.0	41.0	35.0	41.0
38	38.42664662062341	40.0	36.0	41.0	35.0	41.0
39	38.38525467349485	40.0	36.0	41.0	35.0	41.0
40	38.3359539358631	40.0	36.0	41.0	35.0	41.0
41	38.296357780109524	40.0	36.0	41.0	35.0	41.0
42	38.24829947953372	40.0	35.0	41.0	35.0	41.0
43	38.18700417799912	40.0	35.0	41.0	35.0	41.0
44	37.995820260772575	40.0	35.0	41.0	35.0	41.0
45	38.01436807110777	40.0	35.0	41.0	35.0	41.0
46	37.98052297203473	40.0	35.0	41.0	35.0	41.0
47	37.89549955888264	40.0	35.0	41.0	35.0	41.0
48	37.840807966910106	40.0	35.0	41.0	35.0	41.0
49	37.80575871315539	40.0	35.0	41.0	35.0	41.0
50	37.73204600149301	40.0	35.0	41.0	35.0	41.0
51	37.633345340077994	40.0	35.0	41.0	35.0	41.0
52	37.152724051945704	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	3.0
15	3.0
16	8.0
17	19.0
18	32.0
19	70.0
20	133.0
21	199.0
22	317.0
23	516.0
24	825.0
25	1552.0
26	2201.0
27	2571.0
28	2621.0
29	2580.0
30	2965.0
31	3728.0
32	4789.0
33	6868.0
34	20544.0
35	85411.0
36	17012.0
37	27936.0
38	56725.0
39	332991.0
40	2056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.722127386340503	23.163620607750094	25.762123766916023	20.352128238993384
2	25.979985278686986	24.252928166604892	27.313429978927296	22.45365657578083
3	25.410099934397934	25.243571606311022	26.624695263600252	22.721633195690796
4	25.479008208089066	27.598807678052196	23.886809459922702	23.035374653936035
5	23.289778432058355	34.42055276268234	21.5928251870181	20.696843618241203
6	86.07965866043013	2.163302168000459	8.48389617124054	3.2731430003288806
7	87.11832907876946	1.7143543242551902	5.943860638236783	5.223455958738561
8	74.83038298035244	4.0551475002479656	12.76647577682768	8.347993742571914
9	40.098524910514946	16.81640295331116	17.349920042041006	25.73515209413288
10	32.89204196444263	18.395550892066325	31.374667856900484	17.337739286590555
11	32.79320383450182	18.296538751333358	27.209719546806294	21.700537867358534
12	20.94167680279531	27.12689040974321	31.84223485540573	20.089197932055747
13	16.224592249211295	30.093774415889275	32.27430365231251	21.407329682586916
14	15.657839099180931	24.093882302580404	39.6969080022343	20.551370596004364
15	17.721259072487676	19.781198829951432	41.251172397712104	21.246369699848785
16	17.609718154719957	23.58698886504941	29.211365688900027	29.59192729133061
17	18.815612944314804	23.32005630989234	33.36500329750451	24.499327448288344
18	21.230882739347496	28.747800938614215	29.935772616617683	20.085543705420612
19	23.404103522500467	24.161050468350048	27.145335553711043	25.289510455438446
20	19.649298649502242	31.322290608463536	27.725835556321204	21.302575185713017
21	19.54106393678536	23.860359819516006	28.665841855511882	27.932734388186752
22	17.17764935781317	28.32947899428723	26.573188069124047	27.919683578775555
23	17.323470401634307	23.287864313344713	36.03502489224382	23.353640392777162
24	20.548586423329976	23.498591382637553	31.3069776587544	24.645844535278076
25	17.085075616389727	28.716130974443033	30.042093210620923	24.156700198546314
26	18.144453318994845	26.625913339145296	28.695771711761566	26.5338616300983
27	20.986223565585536	26.67881261995869	31.88382343472942	20.45114037972635
28	18.211447473972335	25.440203801439765	28.98741379940384	27.36093492518406
29	19.62389307384844	29.916805440273407	26.809494724862837	23.64980676101532
30	26.61651675636923	26.393608931625938	22.729985713713965	24.259888598290864
31	24.9256538890542	27.250438072169235	22.883115210805375	24.940792827971194
32	23.670862066865386	29.810136824685866	23.175801363200545	23.3431997452482
33	20.08745782413425	28.95591784602481	24.850481226845687	26.10614310299525
34	22.979169168071802	25.29090254177564	26.802882314761163	24.927045975391394
35	23.639888145862805	28.135804982625025	23.320926363853086	24.903380507659083
36	26.008175027015174	29.82196955855202	22.99047986956151	21.17937554487129
37	22.042469073931965	29.127318476291897	24.576588240002646	24.253624209773488
38	22.57006979572873	26.784437170793336	23.023019887693437	27.622473145784504
39	26.326440765856297	24.283728076815326	24.95366962659024	24.436161530738136
40	20.772016280449712	22.44530405775766	28.60493807825961	28.177741583533013
41	22.511428158774407	25.817111177235212	23.23235487064908	28.4391057933413
42	22.014975368772372	21.98434946935409	28.123798237966717	27.876876923906817
43	23.988953794914362	25.956841843331123	26.26710308573338	23.78710127602114
44	19.412469961387004	29.106611192026126	26.645576558658167	24.835342287928697
45	22.024197940756284	26.59041513754683	25.899244271129696	25.48614265056719
46	25.020141749191282	22.229878697076792	26.21455182650428	26.535427727227646
47	21.785455133927407	21.080537414930475	30.737440336049644	26.396567115092477
48	22.53439758333812	20.264426799750122	27.08303969012158	30.11813592679018
49	21.181985706753533	22.897906128138068	29.988845908223226	25.931262256885173
50	20.807688492840327	23.398535177151686	28.635737988470044	27.158038341537942
51	20.071448831256514	20.93402032794074	29.48038637356289	29.514144467239856
52	21.4242087294254	20.229624641320253	34.01301948746862	24.333147141785734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	51.0
1	152.5
2	254.0
3	1482.0
4	2710.0
5	1817.0
6	924.0
7	938.0
8	952.0
9	973.5
10	995.0
11	959.5
12	924.0
13	933.5
14	855.5
15	768.0
16	730.5
17	693.0
18	688.5
19	684.0
20	790.0
21	896.0
22	1046.5
23	1197.0
24	1378.0
25	1559.0
26	1915.0
27	2271.0
28	2600.5
29	2930.0
30	3746.5
31	4563.0
32	5306.0
33	6049.0
34	7056.0
35	8063.0
36	8824.5
37	9586.0
38	11413.0
39	14870.0
40	16500.0
41	19985.5
42	23471.0
43	30148.5
44	36826.0
45	40183.0
46	43540.0
47	48921.0
48	54302.0
49	64204.0
50	74106.0
51	75318.0
52	76530.0
53	68092.5
54	59655.0
55	49790.0
56	39925.0
57	33483.5
58	27042.0
59	23851.0
60	20660.0
61	17821.0
62	14982.0
63	13241.0
64	8758.5
65	6017.0
66	4879.0
67	3741.0
68	3089.0
69	2437.0
70	1876.0
71	1315.0
72	1058.5
73	802.0
74	646.0
75	490.0
76	448.0
77	406.0
78	249.5
79	93.0
80	67.0
81	41.0
82	33.0
83	25.0
84	13.5
85	2.0
86	9.5
87	17.0
88	8.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	574677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.85187046338137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.44694884499283	23.47165908459539
2	12.059880789697132	7.92379283013903
3	4.230079747035393	4.168980956981391
4	1.9987178281699578	2.6264647673556154
5	1.192318088261104	1.9584939693350147
6	0.840931435864487	1.657570235976325
7	0.6584260301820906	1.5141368657282326
8	0.5616599598534705	1.4761264196459352
9	0.4991085919921102	1.4756985730156922
>10	6.2334580593332545	42.088158479153385
>50	0.20247577858693602	4.420505192356668
>100	0.06908622366482617	4.178180500137068
>500	0.003188594938376593	0.7470514106671617
>1k	0.003720027428106025	2.2931807149131034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	3075	0.535083185859187	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTT	2615	0.45503822147049555	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	2252	0.39187230392028916	No Hit
TGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTTG	1518	0.2641483824826816	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1390	0.24187500108756743	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1274	0.22168974919824527	No Hit
ACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1011	0.17592491086297174	No Hit
TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	997	0.1734887597728811	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	718	0.12493974876321828	No Hit
ACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	686	0.11937140341443976	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	685	0.11919739262229044	No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	666	0.11589118757145318	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4621726639486181	0.0
2	0.0	0.0	0.0	1.7053057630634252	0.0
3	0.0	0.0	0.0	2.7128282496080405	0.0
4	0.0	0.0	0.0	3.309337245095941	0.0
5	0.0	0.0	0.0	6.794077368678406	0.0
6	0.0	0.0	0.0	7.385888072778274	0.0
7	0.0	0.0	0.0	10.083751394261473	0.0
8	0.0	0.0	0.0	12.87244834924662	0.0
9	0.0	0.0	0.0	15.514454206449884	0.0
10	0.0	0.0	0.0	17.00416059804029	0.0
11	0.0	0.0	0.0	18.31689801401483	0.0
12	0.0	0.0	0.0	19.33642724521775	0.0
13	0.0	0.0	0.0	19.743786509639328	0.0
14	0.0	0.0	0.0	20.06309631323335	0.0
15	0.0	0.0	0.0	21.27595153451417	0.0
16	0.0	0.0	0.0	22.700577889840726	0.0
17	0.0	0.0	0.0	24.25588635007143	0.0
18	0.0	0.0	0.0	25.119153889924252	0.0
19	0.0	0.0	0.0	25.98120335423203	0.0
20	0.0	0.0	0.0	27.15090389905982	0.0
21	0.0	0.0	0.0	28.2059313318612	0.0
22	0.0	0.0	0.0	29.318382326071863	0.0
23	0.0	0.0	0.0	30.149805890961357	0.0
24	0.0	0.0	0.0	30.872298699965373	0.0
25	0.0	0.0	0.0	31.546590519544022	0.0
26	0.0	0.0	0.0	32.186080180692805	0.0
27	0.0	0.0	0.0	32.829050057684576	0.0
28	0.0	0.0	0.0	33.49324925131857	0.0
29	0.0	0.0	0.0	34.26081085548926	0.0
30	0.0	0.0	0.0	34.93771283695015	0.0
31	0.0	0.0	0.0	35.52186706619545	0.0
32	0.0	0.0	0.0	36.146217788427236	0.0
33	0.0	0.0	0.0	36.74429288104448	0.0
34	0.0	0.0	0.0	37.46591563608775	0.0
35	0.0	0.0	0.0	38.05111393008595	0.0
36	0.0	0.0	0.0	38.643794688146556	0.0
37	0.0	0.0	0.0	39.233691273532784	0.0
38	0.0	0.0	0.0	39.83054829060498	0.0
39	0.0	0.0	0.0	40.47160404888311	0.0
40	0.0	0.0	0.0	41.09108246893472	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	45	3.092282E-10	46.0	1
CGTTGGA	20	6.308808E-4	46.0	27
TAACGTT	20	6.308808E-4	46.0	46
CACGACG	20	6.308808E-4	46.0	25
ATATCGG	40	5.6024874E-9	46.0	1
CTACGAA	40	5.6024874E-9	46.0	11
CTTGCAC	20	6.308808E-4	46.0	23
ACGGATC	20	6.308808E-4	46.0	4
GCGATGC	65	0.0	46.0	8
GGGTTCG	45	3.092282E-10	46.0	6
ACGTAGG	105	0.0	46.0	1
CCAACGG	25	3.415008E-5	46.0	1
ACGAGTC	40	5.6024874E-9	46.0	39
CGATCTA	25	3.415008E-5	46.0	9
TCTGGTA	20	6.308808E-4	46.0	21
CGATACG	20	6.308808E-4	46.0	9
TCAACGG	40	5.6024874E-9	46.0	1
CTAGCGG	185	0.0	46.0	1
ACCGGGT	35	1.0183794E-7	45.999996	3
CTTGCGG	115	0.0	44.000004	1
>>END_MODULE