FastQCFastQC Report
Thu 26 May 2016
SRR1544914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544914_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences646828
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT27710.4283982758940553Illumina PCR Primer Index 5 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT23530.3637752230886727Illumina PCR Primer Index 5 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT23400.3617654152263044Illumina PCR Primer Index 5 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.18227411305633026No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT9720.1502717878632341Illumina PCR Primer Index 5 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC9270.14331476064734364Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC7370.11394064573580613Illumina PCR Primer Index 5 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC6990.1080658227534986Illumina PCR Primer Index 5 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC6710.10373700581916676Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCG206.3093967E-446.00000440
ATCTCCG206.3093967E-446.00000437
ATGATGC405.6043064E-946.00000439
CGTTGAT206.3093967E-446.00000424
TGCGGTC206.3093967E-446.00000416
ATATGCG206.3093967E-446.00000418
GGATACG405.6043064E-946.0000048
TATGCGT206.3093967E-446.00000419
GTAATCG206.3093967E-446.0000049
ATACTAG206.3093967E-446.00000444
GACTTAG206.3093967E-446.0000049
TTGGTCA206.3093967E-446.00000428
ATAAGTC206.3093967E-446.00000411
GTCCGCC206.3093967E-446.0000048
TCGTATT206.3093967E-446.00000422
ATGACGG405.6043064E-946.0000041
GCGATGC1100.046.0000048
TGCTATA206.3093967E-446.00000421
GGTTGCC206.3093967E-446.0000046
GGTCTAA206.3093967E-446.00000431
Produced by FastQC (version 0.11.5)