##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544914_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	646828
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91788079674968	34.0	33.0	34.0	31.0	34.0
2	33.196809661919396	34.0	33.0	34.0	31.0	34.0
3	33.243877816050016	34.0	33.0	34.0	31.0	34.0
4	36.5365460369681	37.0	37.0	37.0	35.0	37.0
5	36.522539222173435	37.0	37.0	37.0	35.0	37.0
6	36.65191828430433	37.0	37.0	37.0	35.0	37.0
7	36.488592021371986	37.0	37.0	37.0	35.0	37.0
8	36.52344054369941	37.0	37.0	37.0	35.0	37.0
9	38.3175079000909	39.0	39.0	39.0	37.0	39.0
10	38.1026810836884	39.0	39.0	39.0	35.0	39.0
11	37.23584940664288	39.0	35.0	39.0	35.0	39.0
12	37.10799006845715	39.0	35.0	39.0	35.0	39.0
13	37.078476813001295	39.0	35.0	39.0	35.0	39.0
14	38.33081437414583	40.0	35.0	41.0	35.0	41.0
15	38.415694744197836	40.0	35.0	41.0	35.0	41.0
16	38.43236532741316	40.0	35.0	41.0	35.0	41.0
17	38.51321062167995	40.0	36.0	41.0	35.0	41.0
18	38.49789279375661	40.0	36.0	41.0	35.0	41.0
19	38.38658190430841	40.0	35.0	41.0	35.0	41.0
20	38.282582077461086	40.0	35.0	41.0	35.0	41.0
21	38.240493608811	40.0	35.0	41.0	35.0	41.0
22	38.20637634734427	40.0	35.0	41.0	35.0	41.0
23	38.20610734229192	40.0	35.0	41.0	35.0	41.0
24	38.143957899163304	40.0	35.0	41.0	35.0	41.0
25	38.106181241381016	40.0	35.0	41.0	35.0	41.0
26	38.07123995869072	40.0	35.0	41.0	35.0	41.0
27	38.08163066533916	40.0	35.0	41.0	35.0	41.0
28	38.09790392500015	40.0	35.0	41.0	35.0	41.0
29	38.1363623714496	40.0	35.0	41.0	35.0	41.0
30	38.03686142220188	40.0	35.0	41.0	35.0	41.0
31	37.93645296740401	40.0	35.0	41.0	35.0	41.0
32	37.85914957299313	40.0	35.0	41.0	35.0	41.0
33	37.78146276908235	40.0	35.0	41.0	35.0	41.0
34	37.664298700736516	40.0	35.0	41.0	35.0	41.0
35	37.6250193250756	40.0	35.0	41.0	35.0	41.0
36	37.563061586696925	40.0	35.0	41.0	35.0	41.0
37	37.501799551039845	40.0	35.0	41.0	35.0	41.0
38	37.46075927449028	39.0	35.0	41.0	35.0	41.0
39	37.3976250255091	39.0	35.0	41.0	34.0	41.0
40	37.34367250644684	39.0	35.0	41.0	34.0	41.0
41	37.30562220559407	39.0	35.0	41.0	34.0	41.0
42	37.18119963885299	39.0	35.0	41.0	34.0	41.0
43	37.090868051475816	38.0	35.0	41.0	34.0	41.0
44	37.010234560037595	38.0	35.0	41.0	34.0	41.0
45	36.977230422925416	38.0	35.0	41.0	34.0	41.0
46	36.99118467351444	38.0	35.0	41.0	34.0	41.0
47	36.95293957589962	38.0	35.0	41.0	34.0	41.0
48	36.99155416895991	38.0	35.0	41.0	34.0	41.0
49	36.89501536730012	38.0	35.0	41.0	34.0	41.0
50	36.806067455335885	37.0	35.0	41.0	34.0	41.0
51	36.726069372383385	37.0	35.0	41.0	34.0	41.0
52	36.54057956674726	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	8.0
15	12.0
16	9.0
17	35.0
18	68.0
19	98.0
20	180.0
21	289.0
22	464.0
23	836.0
24	1431.0
25	2226.0
26	3140.0
27	3683.0
28	3679.0
29	3900.0
30	4660.0
31	5816.0
32	7579.0
33	10904.0
34	32096.0
35	157984.0
36	21630.0
37	33085.0
38	60546.0
39	290377.0
40	2086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.40459596677942	22.250428243675287	24.854057029071097	20.49091876047419
2	26.752397855380412	24.277087571966582	26.898959228728504	22.071555343924505
3	26.008459745094527	24.826692722021928	26.435775816754997	22.729071716128555
4	25.838553680422	27.097157204078982	23.609985962265085	23.454303153233937
5	22.913201036442455	36.06383768173301	21.048872343188606	19.97408893863593
6	86.77453666198743	2.536532122913665	7.891742472496552	2.797188742602361
7	86.43410613022317	2.4944807584087267	5.774950991608279	5.296462119759812
8	76.01371616565764	4.221060312787944	12.662098734130248	7.103124787424169
9	52.851144353676716	16.178334889646088	15.113600524405252	15.856920232271948
10	44.961875490856926	18.561812413810163	24.162373923206786	12.313938172126129
11	42.156029114385895	15.479076354146699	24.611643280748513	17.753251250718893
12	20.204134638574704	35.03883567192515	27.880363867983455	16.876665821516692
13	12.819327549209373	39.02413006239681	29.549586597982767	18.606955790411053
14	11.33454334073355	21.793274255288885	49.07363317605299	17.79854922792458
15	13.071171934424608	16.6603795754049	50.04251516631933	20.225933323851166
16	14.074993661375204	19.73693161087646	28.978028162046172	37.210046565702164
17	18.450654578960712	21.97987718527955	35.38854223997724	24.180925995782495
18	24.45812488018453	26.684373589269484	27.949006536513572	20.908494994032417
19	27.982400267149842	21.365339781209226	25.118887865089327	25.533372086551605
20	18.907808567347114	31.250811653175187	26.976723332941678	22.86465644653602
21	19.10878935358395	23.52526483083602	25.367176436394217	31.99876937918581
22	16.691763498178805	31.28977100558417	22.71654906713995	29.30191642909707
23	17.740110199311097	21.66263674423494	38.314049484561586	22.283203571892372
24	17.51392951449226	21.405226737246995	36.042502798270945	25.038340949989795
25	14.823878991014613	30.80726251801097	30.25781196856042	24.111046522413996
26	18.253075006029423	29.477233514937513	27.170128689543432	25.09956278948963
27	18.235759738292096	26.372234968183193	36.48450592738719	18.90749936613752
28	15.477066546284329	24.853747827861504	32.408770183108956	27.260415442745213
29	21.00697557928847	27.41439764512359	28.290828473720993	23.287798301866957
30	27.559567613028502	23.890586059972666	22.213014897314277	26.336831429684555
31	31.48812358153945	26.45015367300117	21.788790837749758	20.272931907709623
32	26.44938066997718	24.93444934356583	24.609169671071754	24.007000315385234
33	21.15678356533731	25.82108381207987	26.230775414793424	26.791357207789396
34	17.664665104169888	26.284731025867774	30.108931586140365	25.941672283821976
35	29.68223391689908	21.92267496150445	29.396686599837977	18.998404521758488
36	22.897895576567496	28.11628439090454	29.91150661381387	19.074313418714095
37	21.045007328068667	29.644820570538073	27.71092160512532	21.599250496267942
38	18.219217473578755	28.734532209490006	30.219007216756232	22.827243100175007
39	26.190733858150857	21.270878811677914	30.565003370293187	21.973383959878053
40	16.451668758928186	19.232933639236396	32.87999282653194	31.435404775303482
41	23.131033288602225	23.69223348401739	24.88930596696494	28.28742726041544
42	21.40754574631896	20.733796310611165	29.38540075568776	28.473257187382117
43	23.27929526860309	26.614184914691386	28.828993179021317	21.277526637684208
44	17.070844181142437	31.542388393823394	26.873759330146502	24.513008094887667
45	23.587723475174236	27.176931116154528	26.237887042614112	22.997458366057128
46	23.31176139561058	23.341753912941307	28.64331785265944	24.703166838788672
47	21.89840266655123	18.487913324716924	34.23661313363058	25.377070875101264
48	20.535134533446296	18.194481376811147	30.76613875713482	30.504245332607745
49	20.317302281286526	24.664207486379684	31.803045013512094	23.215445218821696
50	19.1627449646583	25.79928512680342	28.485470635161125	26.552499273377155
51	19.27776781462769	21.13111986494091	32.768989592287284	26.82212272814411
52	22.26109568540632	18.267762063485193	35.16514436604476	24.305997885063725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	187.5
2	229.0
3	1203.0
4	2177.0
5	1538.0
6	899.0
7	1004.0
8	1109.0
9	1176.5
10	1244.0
11	1194.5
12	1145.0
13	1132.5
14	1061.0
15	1002.0
16	930.0
17	858.0
18	845.5
19	833.0
20	797.5
21	762.0
22	794.0
23	826.0
24	901.5
25	977.0
26	1343.0
27	1709.0
28	2107.5
29	2506.0
30	3144.5
31	3783.0
32	3941.0
33	4099.0
34	5548.5
35	6998.0
36	7583.5
37	8169.0
38	9822.0
39	14467.5
40	17460.0
41	28344.5
42	39229.0
43	56984.0
44	74739.0
45	85904.0
46	97069.0
47	94862.0
48	92655.0
49	79277.0
50	65899.0
51	57040.5
52	48182.0
53	40751.0
54	33320.0
55	29215.5
56	25111.0
57	23829.5
58	22548.0
59	22123.5
60	21699.0
61	19799.5
62	17900.0
63	16012.5
64	11693.0
65	9261.0
66	7515.5
67	5770.0
68	4663.0
69	3556.0
70	3132.0
71	2708.0
72	2060.0
73	1412.0
74	1149.5
75	887.0
76	748.5
77	610.0
78	485.5
79	361.0
80	247.0
81	133.0
82	112.5
83	92.0
84	61.5
85	31.0
86	16.5
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	646828.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.252567408425513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.9050764690787	16.711250118490454
2	12.725618998970248	6.172578651729326
3	5.080343625706607	3.6963412872124297
4	2.4697981041442727	2.3959578002384205
5	1.4298438565088705	1.733869225675224
6	0.8751241158882097	1.273440396679055
7	0.616341168019064	1.0463499006779045
8	0.45968416521169314	0.8918816962705918
9	0.3523006419264625	0.76897755597178
>10	5.710901814170586	37.92760232365815
>50	1.1712445666736382	17.813340022581116
>100	0.19605893237121422	7.331269501429033
>500	0.005109027553647276	0.8984808885323409
>1k	0.002554513776823638	1.3386606308541669
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	2771	0.4283982758940553	Illumina PCR Primer Index 5 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	2353	0.3637752230886727	Illumina PCR Primer Index 5 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT	2340	0.3617654152263044	Illumina PCR Primer Index 5 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1179	0.18227411305633026	No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	972	0.1502717878632341	Illumina PCR Primer Index 5 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	927	0.14331476064734364	Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	737	0.11394064573580613	Illumina PCR Primer Index 5 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	699	0.1080658227534986	Illumina PCR Primer Index 5 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	671	0.10373700581916676	Illumina PCR Primer Index 5 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5460060479756597E-4	0.0	0.0	0.37088685090936074	0.0
2	1.5460060479756597E-4	0.0	0.0	1.4363942191741854	0.0
3	1.5460060479756597E-4	0.0	0.0	2.3100422368852307	0.0
4	1.5460060479756597E-4	0.0	0.0	2.8418683173888577	0.0
5	1.5460060479756597E-4	0.0	0.0	5.841738452880827	0.0
6	1.5460060479756597E-4	0.0	0.0	6.401083441038422	0.0
7	1.5460060479756597E-4	0.0	0.0	9.372197864038045	0.0
8	1.5460060479756597E-4	0.0	0.0	12.344703692480845	0.0
9	1.5460060479756597E-4	0.0	0.0	15.276085760047494	0.0
10	1.5460060479756597E-4	0.0	0.0	16.457079780096098	0.0
11	1.5460060479756597E-4	0.0	0.0	17.604525468903635	0.0
12	3.0920120959513194E-4	0.0	0.0	18.60200857105753	0.0
13	3.0920120959513194E-4	0.0	0.0	18.954034148181588	0.0
14	3.0920120959513194E-4	0.0	0.0	19.232315236817207	0.0
15	3.0920120959513194E-4	0.0	0.0	20.30106921778278	0.0
16	3.0920120959513194E-4	0.0	0.0	21.33581106569289	0.0
17	4.638018143926979E-4	0.0	0.0	23.11170821300253	0.0
18	4.638018143926979E-4	0.0	0.0	23.71001255356911	0.0
19	4.638018143926979E-4	0.0	0.0	24.60746906441898	0.0
20	4.638018143926979E-4	0.0	0.0	25.52533285510213	0.0
21	4.638018143926979E-4	0.0	0.0	26.24175205773405	0.0
22	4.638018143926979E-4	0.0	0.0	27.128850328062484	0.0
23	4.638018143926979E-4	0.0	0.0	27.704119178514226	0.0
24	6.184024191902639E-4	0.0	0.0	28.200232519309615	0.0
25	7.730030239878298E-4	0.0	0.0	28.711032917560775	0.0
26	7.730030239878298E-4	0.0	0.0	29.14948023276667	0.0
27	9.276036287853958E-4	0.0	0.0	29.627350702195947	0.0
28	9.276036287853958E-4	0.0	0.0	30.079557471228828	0.0
29	9.276036287853958E-4	0.0	0.0	30.729962215612186	0.0
30	9.276036287853958E-4	0.0	0.0	31.347282430568868	0.0
31	9.276036287853958E-4	0.0	0.0	31.823143092135776	0.0
32	9.276036287853958E-4	0.0	0.0	32.31400001236805	0.0
33	9.276036287853958E-4	0.0	0.0	32.76651598261052	0.0
34	9.276036287853958E-4	0.0	0.0	33.44722244553421	0.0
35	9.276036287853958E-4	0.0	0.0	33.93127693915539	0.0
36	9.276036287853958E-4	0.0	0.0	34.40358178681195	0.0
37	9.276036287853958E-4	0.0	0.0	34.83476287359236	0.0
38	9.276036287853958E-4	0.0	0.0	35.26594396037277	0.0
39	9.276036287853958E-4	0.0	0.0	35.75911988967701	0.0
40	9.276036287853958E-4	0.0	0.0	36.27347610183851	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACCG	20	6.3093967E-4	46.000004	40
ATCTCCG	20	6.3093967E-4	46.000004	37
ATGATGC	40	5.6043064E-9	46.000004	39
CGTTGAT	20	6.3093967E-4	46.000004	24
TGCGGTC	20	6.3093967E-4	46.000004	16
ATATGCG	20	6.3093967E-4	46.000004	18
GGATACG	40	5.6043064E-9	46.000004	8
TATGCGT	20	6.3093967E-4	46.000004	19
GTAATCG	20	6.3093967E-4	46.000004	9
ATACTAG	20	6.3093967E-4	46.000004	44
GACTTAG	20	6.3093967E-4	46.000004	9
TTGGTCA	20	6.3093967E-4	46.000004	28
ATAAGTC	20	6.3093967E-4	46.000004	11
GTCCGCC	20	6.3093967E-4	46.000004	8
TCGTATT	20	6.3093967E-4	46.000004	22
ATGACGG	40	5.6043064E-9	46.000004	1
GCGATGC	110	0.0	46.000004	8
TGCTATA	20	6.3093967E-4	46.000004	21
GGTTGCC	20	6.3093967E-4	46.000004	6
GGTCTAA	20	6.3093967E-4	46.000004	31
>>END_MODULE