SRR1544913_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544913_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	396592
Sequences flagged as poor quality	0
Sequence length	52
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	2455	0.6190240852059548	Illumina PCR Primer Index 5 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT	1910	0.48160325977326823	Illumina PCR Primer Index 5 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	1567	0.3951163916569169	Illumina PCR Primer Index 5 (95% over 24bp)
TGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTTG	1115	0.2811453584540283	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	774	0.19516278694476943	No Hit
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	664	0.16742647355468593	Illumina PCR Primer Index 5 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	662	0.1669221769475935	No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	641	0.161627062573123	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	512	0.12909993141566142	Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	487	0.1227962238270061	Illumina PCR Primer Index 5 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	479	0.12077903739863638	Illumina PCR Primer Index 5 (95% over 24bp)
CTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTTGAAA	447	0.11271029168515755	Illumina PCR Primer Index 5 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	436	0.1099366603461492	Illumina PCR Primer Index 5 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	40	5.5952114E-9	46.000004	5
GAGCTTC	20	6.3064287E-4	46.000004	27
CAACGTT	20	6.3064287E-4	46.000004	19
CGGCCTA	20	6.3064287E-4	46.000004	44
TAACGCT	20	6.3064287E-4	46.000004	31
GTTACGG	20	6.3064287E-4	46.000004	1
GCCGATG	20	6.3064287E-4	46.000004	8
TGCGATG	20	6.3064287E-4	46.000004	28
GCCTCGA	40	5.5952114E-9	46.000004	13
GCGATAG	20	6.3064287E-4	46.000004	8
AACCTGG	20	6.3064287E-4	46.000004	1
CCGAAAT	20	6.3064287E-4	46.000004	44
CAACTGG	40	5.5952114E-9	46.000004	1
GGCGATA	40	5.5952114E-9	46.000004	7
ATGCACG	20	6.3064287E-4	46.000004	45
GACCTAC	20	6.3064287E-4	46.000004	20
GTGCGAT	20	6.3064287E-4	46.000004	27
GCCGTGA	20	6.3064287E-4	46.000004	31
GGTAGCC	20	6.3064287E-4	46.000004	7
CGGGTCC	25	3.4130804E-5	46.0	5