FastQCFastQC Report
Thu 26 May 2016
SRR1544913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544913_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences396592
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT24550.6190240852059548Illumina PCR Primer Index 5 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT19100.48160325977326823Illumina PCR Primer Index 5 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT15670.3951163916569169Illumina PCR Primer Index 5 (95% over 24bp)
TGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTTG11150.2811453584540283Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.19516278694476943No Hit
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC6640.16742647355468593Illumina PCR Primer Index 5 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6620.1669221769475935No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT6410.161627062573123Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC5120.12909993141566142Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC4870.1227962238270061Illumina PCR Primer Index 5 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT4790.12077903739863638Illumina PCR Primer Index 5 (95% over 24bp)
CTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTTGAAA4470.11271029168515755Illumina PCR Primer Index 5 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC4360.1099366603461492Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT405.5952114E-946.0000045
GAGCTTC206.3064287E-446.00000427
CAACGTT206.3064287E-446.00000419
CGGCCTA206.3064287E-446.00000444
TAACGCT206.3064287E-446.00000431
GTTACGG206.3064287E-446.0000041
GCCGATG206.3064287E-446.0000048
TGCGATG206.3064287E-446.00000428
GCCTCGA405.5952114E-946.00000413
GCGATAG206.3064287E-446.0000048
AACCTGG206.3064287E-446.0000041
CCGAAAT206.3064287E-446.00000444
CAACTGG405.5952114E-946.0000041
GGCGATA405.5952114E-946.0000047
ATGCACG206.3064287E-446.00000445
GACCTAC206.3064287E-446.00000420
GTGCGAT206.3064287E-446.00000427
GCCGTGA206.3064287E-446.00000431
GGTAGCC206.3064287E-446.0000047
CGGGTCC253.4130804E-546.05