##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544913_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	396592
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91884102553758	34.0	33.0	34.0	31.0	34.0
2	33.246298462903944	34.0	33.0	34.0	31.0	34.0
3	33.368307984023886	34.0	34.0	34.0	31.0	34.0
4	36.6011518134506	37.0	37.0	37.0	35.0	37.0
5	36.590849033767704	37.0	37.0	37.0	35.0	37.0
6	36.41632710695122	37.0	37.0	37.0	35.0	37.0
7	36.24106890708839	37.0	37.0	37.0	35.0	37.0
8	36.38496490095615	37.0	37.0	37.0	35.0	37.0
9	38.26931456005164	39.0	39.0	39.0	37.0	39.0
10	38.13913795537984	39.0	39.0	39.0	35.0	39.0
11	37.35942479928995	39.0	35.0	39.0	35.0	39.0
12	37.308947230403035	39.0	35.0	39.0	35.0	39.0
13	37.29230039940291	39.0	35.0	39.0	35.0	39.0
14	38.499525961189335	41.0	35.0	41.0	35.0	41.0
15	38.543843547020614	41.0	35.0	41.0	35.0	41.0
16	38.587573627304636	41.0	35.0	41.0	35.0	41.0
17	38.68905575503288	41.0	36.0	41.0	35.0	41.0
18	38.68375559769234	40.0	36.0	41.0	35.0	41.0
19	38.60542824867874	41.0	35.0	41.0	35.0	41.0
20	38.51600385282608	40.0	35.0	41.0	35.0	41.0
21	38.479818049784164	40.0	35.0	41.0	35.0	41.0
22	38.443687719369024	40.0	35.0	41.0	35.0	41.0
23	38.42029087828297	40.0	35.0	41.0	35.0	41.0
24	38.38160124258684	40.0	35.0	41.0	35.0	41.0
25	38.34335790938798	40.0	35.0	41.0	35.0	41.0
26	38.28612528744907	40.0	35.0	41.0	35.0	41.0
27	38.387004780731836	40.0	35.0	41.0	35.0	41.0
28	38.39638721910679	40.0	35.0	41.0	35.0	41.0
29	38.43621908661799	40.0	36.0	41.0	35.0	41.0
30	38.344669080566426	40.0	35.0	41.0	35.0	41.0
31	38.25755184169121	40.0	35.0	41.0	35.0	41.0
32	38.182631016258526	40.0	35.0	41.0	35.0	41.0
33	38.139667466817286	40.0	35.0	41.0	35.0	41.0
34	38.03062593294872	40.0	35.0	41.0	35.0	41.0
35	37.985077863396135	40.0	35.0	41.0	35.0	41.0
36	37.93880360672934	40.0	35.0	41.0	35.0	41.0
37	37.88624077137209	40.0	35.0	41.0	35.0	41.0
38	37.83488572638883	40.0	35.0	41.0	35.0	41.0
39	37.80174587485375	40.0	35.0	41.0	35.0	41.0
40	37.762312401662165	40.0	35.0	41.0	35.0	41.0
41	37.74090501069109	40.0	35.0	41.0	35.0	41.0
42	37.65847016581272	40.0	35.0	41.0	35.0	41.0
43	37.57894511235728	40.0	35.0	41.0	35.0	41.0
44	37.43870779037399	39.0	35.0	41.0	35.0	41.0
45	37.43701083229112	39.0	35.0	41.0	35.0	41.0
46	37.3689988703756	39.0	35.0	41.0	35.0	41.0
47	37.30693508694073	39.0	35.0	41.0	35.0	41.0
48	37.2812714326058	39.0	35.0	41.0	35.0	41.0
49	37.20060162585226	39.0	35.0	41.0	35.0	41.0
50	37.11812139427926	38.0	35.0	41.0	35.0	41.0
51	37.03211612942268	38.0	35.0	41.0	34.0	41.0
52	36.61409710735466	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	2.0
15	5.0
16	7.0
17	14.0
18	24.0
19	59.0
20	79.0
21	139.0
22	228.0
23	408.0
24	706.0
25	1223.0
26	1674.0
27	2007.0
28	1892.0
29	1883.0
30	2135.0
31	2482.0
32	3492.0
33	5266.0
34	17467.0
35	92164.0
36	12345.0
37	19394.0
38	37930.0
39	191971.0
40	1593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.52156372291927	21.930850849235487	23.886765239843466	22.660820188001775
2	26.55096421511276	24.675232985032476	26.615766329124135	22.158036470730625
3	26.01565336668415	24.70498648485093	26.551468511719854	22.727891636745067
4	25.943286803566384	26.78218420946464	23.868106265381048	23.40642272158793
5	22.940452656634527	35.42154153386856	21.2805603743898	20.357445435107113
6	85.27680840763303	2.5144228829628434	8.69079557832735	3.5179731310767743
7	84.81487271553637	2.389609472707468	6.698319683705169	6.097198128050994
8	73.86230685439948	4.314761770282809	13.830082301206279	7.9928490741114295
9	51.45666074958648	15.714386573607134	15.94232864001291	16.886624036793478
10	43.034150966232296	18.203090329608262	26.036581675878484	12.726177028280954
11	40.42845039738573	15.450135151490702	25.320732642110784	18.80068180901279
12	19.71295437124299	33.150441763827814	29.385363295275752	17.751240569653447
13	12.756182676402952	36.98107879130189	30.58861499979828	19.674123532496875
14	11.32196312583209	21.553889135433895	48.22462379473111	18.899523944002905
15	13.015895429055554	16.608504457982008	49.378454431758584	20.997145681203857
16	14.079961270020574	20.409892282244723	29.41385605357647	36.096290394158224
17	17.84957840803647	22.171400330818575	35.639649816436034	24.33937144470892
18	23.756404566910074	27.213357808528666	28.104449913260982	20.925787711300277
19	26.979364182837777	21.39200992455723	25.50001008593214	26.12861580667285
20	18.42094646387219	31.907854923952073	26.620557146891517	23.050641465284222
21	18.71066486464679	23.603350546657524	25.415288255940617	32.27069633275507
22	16.68011457618913	31.528876023722113	22.648212772824465	29.14279662726429
23	17.252491225239037	22.05112559002703	37.809638116754755	22.886745067979184
24	17.98825997498689	21.801246621212734	34.98986363819744	25.220629765602936
25	15.091580263847984	30.117854117077503	30.402781700084724	24.387783918989793
26	18.624177996530438	28.26355549279864	26.91405777221931	26.198208738451605
27	19.036188324524954	25.594313551458427	36.02795820389721	19.34153992011942
28	16.217170290878283	24.52394400290475	31.967361923588978	27.29152378262799
29	21.593224270787108	26.893633759632063	27.86818695283818	23.644955016742646
30	27.148051397910194	23.22790172267721	22.12929156412636	27.49475531528624
31	31.775981361197402	26.275870415943842	21.286107637067815	20.66204058579094
32	25.798553677330858	23.952575947069025	25.197432121676684	25.05143825392343
33	21.449247589462217	25.151541130431276	26.46599023681769	26.933221043288817
34	17.969348852220925	25.641465284221564	30.475400411506033	25.91378545205148
35	29.11455601726712	22.083148424577402	29.708113123814904	19.09418243434058
36	23.05795376608706	27.836164118287808	29.851585508532697	19.254296607092428
37	21.338806632508977	29.331151410013312	28.289274619760356	21.04076733771735
38	18.079033364263523	28.800127082744986	30.22350425626336	22.897335296728123
39	26.157612861580663	20.764160648727156	31.277988461693628	21.800238027998546
40	16.75475047403881	18.606527615282204	33.20414935248316	31.43457255819583
41	22.95079073707992	23.659831766651873	25.019415419373058	28.369962076895145
42	21.042784524145723	20.325926897163836	29.88259974986888	28.74868882882156
43	24.082684471698872	25.712318957518054	28.87577157380885	21.32922499697422
44	17.415126881026342	29.77089805139791	28.07167063379998	24.742304433775768
45	23.26118529874531	26.4982652196716	26.470024609674425	23.770524871908663
46	23.35574091257514	21.891767862185823	29.546233912938234	25.2062573123008
47	21.56750514382539	17.675343930286036	34.72082139831363	26.036329527574935
48	20.195566224230443	17.609028926453384	30.66274660104087	31.532658248275307
49	20.177411546375115	23.489127365151088	32.173871384193326	24.15958970428047
50	19.76414047686287	24.534786379957236	29.125398394319603	26.57567474886029
51	18.99710533747529	20.171359987090007	33.17010933150441	27.661425343930286
52	22.0564207044015	17.773933916972606	35.659065235809095	24.5105801428168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	120.0
2	164.0
3	779.5
4	1395.0
5	994.5
6	594.0
7	667.5
8	741.0
9	783.5
10	826.0
11	777.0
12	728.0
13	712.0
14	651.0
15	606.0
16	562.0
17	518.0
18	529.0
19	540.0
20	519.5
21	499.0
22	504.5
23	510.0
24	552.5
25	595.0
26	786.0
27	977.0
28	1172.0
29	1367.0
30	1672.0
31	1977.0
32	2106.5
33	2236.0
34	2953.0
35	3670.0
36	4143.0
37	4616.0
38	5455.5
39	8015.5
40	9736.0
41	15874.0
42	22012.0
43	32919.0
44	43826.0
45	52326.0
46	60826.0
47	59735.0
48	58644.0
49	50582.5
50	42521.0
51	36532.5
52	30544.0
53	25690.0
54	20836.0
55	18136.5
56	15437.0
57	14586.0
58	13735.0
59	13403.0
60	13071.0
61	12029.0
62	10987.0
63	10043.0
64	7459.5
65	5820.0
66	4713.0
67	3606.0
68	2912.0
69	2218.0
70	1982.5
71	1747.0
72	1338.5
73	930.0
74	776.0
75	622.0
76	506.0
77	390.0
78	300.5
79	211.0
80	147.0
81	83.0
82	63.5
83	44.0
84	32.5
85	21.0
86	10.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	396592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.229851417322372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.99579829565336	20.751966354361365
2	11.584704383123725	6.772383756646194
3	4.126511854857186	3.6185198516788466
4	2.0172410815458823	2.3585462834601927
5	1.1578113456135009	1.6921326800786354
6	0.7907753081657842	1.3868546857303912
7	0.5943604213492379	1.2161146763062742
8	0.4870785537917317	1.1389787004717264
9	0.40229357825085266	1.0583083366573838
>10	7.451513463958749	47.663060285799105
>50	0.30626213387694556	5.896685262820507
>100	0.07786325437549463	3.841772129375124
>500	0.004325736354194146	0.8226227446588519
>1k	0.003460589083355317	1.7820542519554041
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	2455	0.6190240852059548	Illumina PCR Primer Index 5 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT	1910	0.48160325977326823	Illumina PCR Primer Index 5 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	1567	0.3951163916569169	Illumina PCR Primer Index 5 (95% over 24bp)
TGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTTG	1115	0.2811453584540283	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	774	0.19516278694476943	No Hit
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	664	0.16742647355468593	Illumina PCR Primer Index 5 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	662	0.1669221769475935	No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	641	0.161627062573123	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	512	0.12909993141566142	Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	487	0.1227962238270061	Illumina PCR Primer Index 5 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	479	0.12077903739863638	Illumina PCR Primer Index 5 (95% over 24bp)
CTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTTGAAA	447	0.11271029168515755	Illumina PCR Primer Index 5 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	436	0.1099366603461492	Illumina PCR Primer Index 5 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4896720054867471	0.0
2	0.0	0.0	0.0	1.8129463024972767	0.0
3	0.0	0.0	0.0	2.8170008472183	0.0
4	0.0	0.0	0.0	3.4355206358171624	0.0
5	0.0	0.0	0.0	6.782032920482511	0.0
6	0.0	0.0	0.0	7.435853471577843	0.0
7	0.0	0.0	0.0	10.811867107758099	0.0
8	0.0	0.0	0.0	14.089795053858877	0.0
9	0.0	0.0	0.0	17.563390083511518	0.0
10	0.0	0.0	0.0	18.929529592124904	0.0
11	0.0	0.0	0.0	20.215485940210595	0.0
12	0.0	0.0	0.0	21.401339411788438	0.0
13	0.0	0.0	0.0	21.81208899826522	0.0
14	0.0	0.0	0.0	22.12525719126962	0.0
15	0.0	0.0	0.0	23.254125146246015	0.0
16	0.0	0.0	0.0	24.380975914794046	0.0
17	0.0	0.0	0.0	26.090541412837375	0.0
18	0.0	0.0	0.0	26.73881470125469	0.0
19	0.0	0.0	0.0	27.602170492596926	0.0
20	0.0	0.0	0.0	28.606477185621497	0.0
21	0.0	0.0	0.0	29.38813692661476	0.0
22	2.521483035462137E-4	0.0	0.0	30.335458103037883	0.0
23	2.521483035462137E-4	0.0	0.0	30.960029450921855	0.0
24	7.564449106386412E-4	0.0	0.0	31.47844636301287	0.0
25	7.564449106386412E-4	0.0	0.0	32.00165409287126	0.0
26	7.564449106386412E-4	0.0	0.0	32.48502239076935	0.0
27	7.564449106386412E-4	0.0	0.0	32.99310122241498	0.0
28	7.564449106386412E-4	0.0	0.0	33.464618550046396	0.0
29	7.564449106386412E-4	0.0	0.0	34.08187759712753	0.0
30	7.564449106386412E-4	0.0	0.0	34.70241457215476	0.0
31	7.564449106386412E-4	0.0	0.0	35.16687174728688	0.0
32	7.564449106386412E-4	0.0	0.0	35.63738048170412	0.0
33	7.564449106386412E-4	0.0	0.0	36.08393512728446	0.0
34	7.564449106386412E-4	0.0	0.0	36.74355508936136	0.0
35	7.564449106386412E-4	0.0	0.0	37.219358938153064	0.0
36	7.564449106386412E-4	0.0	0.0	37.683563964981644	0.0
37	7.564449106386412E-4	0.0	0.0	38.121041271634326	0.0
38	7.564449106386412E-4	0.0	0.0	38.57062169685722	0.0
39	7.564449106386412E-4	0.0	0.0	39.058024367612056	0.0
40	7.564449106386412E-4	0.0	0.0	39.552235042562636	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	40	5.5952114E-9	46.000004	5
GAGCTTC	20	6.3064287E-4	46.000004	27
CAACGTT	20	6.3064287E-4	46.000004	19
CGGCCTA	20	6.3064287E-4	46.000004	44
TAACGCT	20	6.3064287E-4	46.000004	31
GTTACGG	20	6.3064287E-4	46.000004	1
GCCGATG	20	6.3064287E-4	46.000004	8
TGCGATG	20	6.3064287E-4	46.000004	28
GCCTCGA	40	5.5952114E-9	46.000004	13
GCGATAG	20	6.3064287E-4	46.000004	8
AACCTGG	20	6.3064287E-4	46.000004	1
CCGAAAT	20	6.3064287E-4	46.000004	44
CAACTGG	40	5.5952114E-9	46.000004	1
GGCGATA	40	5.5952114E-9	46.000004	7
ATGCACG	20	6.3064287E-4	46.000004	45
GACCTAC	20	6.3064287E-4	46.000004	20
GTGCGAT	20	6.3064287E-4	46.000004	27
GCCGTGA	20	6.3064287E-4	46.000004	31
GGTAGCC	20	6.3064287E-4	46.000004	7
CGGGTCC	25	3.4130804E-5	46.0	5
>>END_MODULE