FastQCFastQC Report
Thu 26 May 2016
SRR1544912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544912_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1158209
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT43180.37281699589625017Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTT32670.2820734427033463Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT30220.26092009300566654Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.23622679499123214No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA15040.12985566508289956No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14760.1274381394031647No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG13740.11863143871270211No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT13690.11819973769846374No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13340.1151778305987952No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT13000.11224226370197435No Hit
ACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC12470.1076662329510477Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT551.8189894E-1246.0000043
AGGTACG206.311474E-446.010
CTATATG301.8614155E-646.041
TTCGTAC206.311474E-446.028
ATTCGAT301.8614155E-646.013
TTATCGG206.311474E-446.01
ATACTCG301.8614155E-646.043
CTAACGA206.311474E-446.025
TCGTGCA206.311474E-446.018
GTCGTGC206.311474E-446.017
TCGTACT206.311474E-446.029
TCGTAAT206.311474E-446.045
ATACGGT206.311474E-446.021
AGTACGG850.046.01
TACACCG253.4171724E-546.018
AGTCGAC206.311474E-446.012
GTTGCGA206.311474E-446.08
TACCGAA405.6115823E-946.08
GCGAACT351.01957994E-745.99999622
CTAACGG1200.044.0833321
Produced by FastQC (version 0.11.5)