FastQCFastQC Report
Thu 26 May 2016
SRR1544911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544911_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences729827
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT34670.47504408579019414Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTT27020.37022472448950233Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT25230.3456983641328698Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA21050.2884245170430801No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18130.2484150353439925No Hit
TGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTTG16830.230602594861522Illumina Single End Adapter 1 (95% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9350.12811255270084554No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8650.11852123859489988No Hit
ACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC8620.11811018227607364Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC8120.1112592436289696Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC7990.10947799958072255Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT7790.10673762412188094Illumina Single End Adapter 1 (95% over 22bp)
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT7570.10372321111715517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG551.8189894E-1246.00000419
ATTCGTC206.3099316E-446.00000435
ATAAGCG405.6061253E-946.00000432
ACCGTTA206.3099316E-446.00000425
CGGTCTA351.0188887E-746.00000430
ATAACGG351.0188887E-746.0000041
CGACTAA206.3099316E-446.00000413
GCCAACG206.3099316E-446.00000445
TAATCGT551.8189894E-1246.00000420
ATTCGAT301.8604624E-646.013
TATTGTC253.4159217E-546.06
CGTAGGG2550.046.02
GTCAAGC501.6370905E-1146.015
CGGTATT253.4159217E-546.043
AGTACGG501.6370905E-1146.01
TAGTCAA253.4159217E-546.011
TATCCGC253.4159217E-546.029
AACACGG253.4159217E-546.01
ATTGCGG1550.044.516131
ACGGGAT1200.044.0833324
Produced by FastQC (version 0.11.5)