##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544911_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	729827
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90731776160652	34.0	33.0	34.0	31.0	34.0
2	33.279355244462046	34.0	34.0	34.0	31.0	34.0
3	33.40374362691433	34.0	34.0	34.0	31.0	34.0
4	36.61420035159017	37.0	37.0	37.0	35.0	37.0
5	36.6257099285173	37.0	37.0	37.0	35.0	37.0
6	36.40966694846861	37.0	37.0	37.0	35.0	37.0
7	36.2081260353481	37.0	37.0	37.0	35.0	37.0
8	36.3553869615676	37.0	37.0	37.0	35.0	37.0
9	38.24978111251023	39.0	39.0	39.0	37.0	39.0
10	38.142855772669414	39.0	39.0	39.0	35.0	39.0
11	37.61874526428866	39.0	35.0	39.0	35.0	39.0
12	37.59584120620366	39.0	35.0	39.0	35.0	39.0
13	37.597703291327946	39.0	35.0	39.0	35.0	39.0
14	38.86786046556239	41.0	36.0	41.0	35.0	41.0
15	38.9043129399159	41.0	36.0	41.0	35.0	41.0
16	38.92978884036902	41.0	36.0	41.0	35.0	41.0
17	39.00633574806084	41.0	36.0	41.0	35.0	41.0
18	38.994953598592545	41.0	36.0	41.0	35.0	41.0
19	38.943545525172404	41.0	36.0	41.0	35.0	41.0
20	38.874779913595965	41.0	35.0	41.0	35.0	41.0
21	38.812465145849636	41.0	35.0	41.0	35.0	41.0
22	38.776241492846935	41.0	35.0	41.0	35.0	41.0
23	38.73966022084686	41.0	35.0	41.0	35.0	41.0
24	38.69487426472301	41.0	35.0	41.0	35.0	41.0
25	38.64382381030025	41.0	35.0	41.0	35.0	41.0
26	38.56947468372642	40.0	35.0	41.0	35.0	41.0
27	38.64621752826355	41.0	36.0	41.0	35.0	41.0
28	38.66461777928194	41.0	36.0	41.0	35.0	41.0
29	38.67913491827515	41.0	36.0	41.0	35.0	41.0
30	38.62824066525355	41.0	35.0	41.0	35.0	41.0
31	38.55474242525968	41.0	35.0	41.0	35.0	41.0
32	38.506117203118	41.0	35.0	41.0	35.0	41.0
33	38.42478559987504	40.0	35.0	41.0	35.0	41.0
34	38.310725692527136	40.0	35.0	41.0	35.0	41.0
35	38.24778885955165	40.0	35.0	41.0	35.0	41.0
36	38.140600443666784	40.0	35.0	41.0	35.0	41.0
37	38.10631697648895	40.0	35.0	41.0	35.0	41.0
38	38.00279107240483	40.0	35.0	41.0	35.0	41.0
39	37.91844916672033	40.0	35.0	41.0	35.0	41.0
40	37.862334498449634	40.0	35.0	41.0	35.0	41.0
41	37.831273712811395	40.0	35.0	41.0	35.0	41.0
42	37.76776140098955	40.0	35.0	41.0	35.0	41.0
43	37.70227601883734	40.0	35.0	41.0	35.0	41.0
44	37.58587035009667	40.0	35.0	41.0	35.0	41.0
45	37.57365512648888	40.0	35.0	41.0	35.0	41.0
46	37.503716634216055	39.0	35.0	41.0	35.0	41.0
47	37.41716735609946	39.0	35.0	41.0	35.0	41.0
48	37.349166309276036	39.0	35.0	41.0	35.0	41.0
49	37.30119466668128	39.0	35.0	41.0	35.0	41.0
50	37.23593125494124	39.0	35.0	41.0	35.0	41.0
51	37.1535898233417	39.0	35.0	41.0	34.0	41.0
52	36.72132710902721	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	4.0
14	2.0
15	7.0
16	14.0
17	26.0
18	48.0
19	104.0
20	159.0
21	273.0
22	406.0
23	670.0
24	1176.0
25	1977.0
26	2680.0
27	3390.0
28	3407.0
29	3296.0
30	3706.0
31	4676.0
32	6068.0
33	8903.0
34	32042.0
35	131496.0
36	28009.0
37	46505.0
38	80693.0
39	366972.0
40	3115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.02892329278034	22.182380207912285	22.772109006655004	22.016587492652366
2	28.343703370798835	23.750971120553228	25.107868028998652	22.797457479649285
3	28.25066762397116	24.102972348241433	25.15911305007899	22.487246977708416
4	26.960361839175583	26.04178798537188	22.14976973995207	24.84808043550047
5	24.790395532091853	35.762584831747795	19.987339465380153	19.4596801707802
6	85.52698105167389	3.2707751289004108	7.87090639288489	3.3313374265408107
7	85.12072038990061	3.248167031364967	6.299027029693338	5.332085549041074
8	73.0812918677988	6.287928509085029	12.464460755768147	8.166318867348016
9	49.43746942768629	19.862515363229917	15.627676147909025	15.072339061174771
10	38.26139619389253	20.705180816823713	25.912305244941606	15.121117744342152
11	36.34244827883869	17.676928916030786	25.132805445674112	20.847817359456418
12	20.942223294013512	29.87639536492895	29.64414854479212	19.537232796265418
13	15.758940132387538	32.17228192434645	30.49092456157418	21.577853381691824
14	14.034284837365568	24.166138002567735	40.63716469793526	21.162412462131435
15	16.2224746412506	18.20856175504606	43.21859838016407	22.350365223539274
16	17.285603300508203	22.01672451142531	28.303693889099748	32.39397829896674
17	19.257577480690628	23.466383129152526	31.572002680087195	25.704036710069644
18	23.776593631093395	26.23580656785786	27.672037345836753	22.31556245521199
19	25.97875934981852	22.745253327158355	25.772409077767744	25.503578245255383
20	21.351087312472682	29.588518922977634	27.08176047200227	21.97863329254741
21	21.3879453623941	23.847021280385626	25.3268240281601	29.43820932906017
22	19.44597829348599	29.01906890263035	23.91292731017077	27.622025493712894
23	19.06122957906463	22.258836683213968	35.38646830002178	23.29346543769962
24	19.867448039055834	22.597547089926792	32.08733028512236	25.447674585895015
25	17.96686063957623	28.29232133094555	28.741057812330865	24.99976021714735
26	19.26634668215892	26.932547028268345	27.533237328846425	26.26786896072631
27	21.149258659928996	24.34179606947948	33.309400720992784	21.19954454959874
28	19.33403395599231	23.341833064548172	29.799116776989614	27.5250162024699
29	20.528974674820198	25.12458431929759	28.686798378245804	25.659642627636416
30	25.179802884793247	23.92621813114615	23.051216247138022	27.842762736922587
31	28.406869025125133	23.591070212529818	24.679821382327592	23.322239380017457
32	23.67999539616923	23.737132224486075	25.46452789496689	27.118344484377804
33	20.08804826349258	21.94958531268369	30.131661338920047	27.830705084903684
34	21.802290131770956	22.99723085059884	28.212302367547377	26.988176650082828
35	29.984229139234365	20.357838227415538	25.705406897799065	23.952525735551028
36	21.627043121178033	26.233477248717847	27.869618416419232	24.269861213684887
37	23.068206574982838	26.856227571739606	27.65230664253309	22.423259210744465
38	22.71921976029936	25.692527133142512	26.050283149294284	25.537969957263844
39	27.10012098757651	21.45960618064281	29.15636171311831	22.283911118662367
40	17.96713467712211	19.93773866957512	32.881628111867606	29.21349854143516
41	22.230884853533784	23.80920409905361	25.52878969947672	28.43112134793588
42	22.817188182952947	20.730118233499173	28.54758730493665	27.905106278611232
43	23.526671389247042	24.799164733560144	28.193530795654315	23.480633081538503
44	18.90859066600715	26.877465481545627	27.85687567053562	26.357068181911604
45	22.416271253324418	25.637993661511565	27.00653716565707	24.939197919506952
46	23.777004687412223	21.46823836333816	28.894518838025995	25.860238111223623
47	22.922281581799524	19.33143059930641	32.2424355360928	25.503852282801265
48	21.191186404449276	19.19386375127256	29.668811923921695	29.94613792035647
49	21.015939393856353	22.734017787777102	31.13628298213138	25.11375983623516
50	20.636945467898556	24.028297116988	28.668437862671563	26.66631955244188
51	20.65558002101868	21.094451150752164	30.63643849843867	27.613530329790485
52	23.02860814960258	18.6958005116281	32.94767116042569	25.327920178343632
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	286.5
2	465.0
3	1853.0
4	3241.0
5	2243.0
6	1245.0
7	1236.0
8	1227.0
9	1219.5
10	1212.0
11	1200.0
12	1188.0
13	1151.5
14	1051.5
15	988.0
16	987.0
17	986.0
18	1100.0
19	1214.0
20	1201.0
21	1188.0
22	1390.5
23	1593.0
24	1536.0
25	1479.0
26	2044.0
27	2609.0
28	3727.0
29	4845.0
30	5581.5
31	6318.0
32	6647.0
33	6976.0
34	8343.5
35	9711.0
36	10109.0
37	10507.0
38	12359.0
39	17691.0
40	21171.0
41	26852.5
42	32534.0
43	42263.0
44	51992.0
45	59308.0
46	66624.0
47	74381.0
48	82138.0
49	83318.0
50	84498.0
51	75806.0
52	67114.0
53	57705.5
54	48297.0
55	41932.5
56	35568.0
57	32364.0
58	29160.0
59	28402.0
60	27644.0
61	27842.0
62	28040.0
63	27266.0
64	21736.0
65	16980.0
66	14255.0
67	11530.0
68	10035.5
69	8541.0
70	7358.5
71	6176.0
72	5401.5
73	4627.0
74	4125.5
75	3624.0
76	3036.5
77	2449.0
78	1804.0
79	1159.0
80	899.5
81	640.0
82	433.0
83	226.0
84	176.0
85	126.0
86	70.5
87	15.0
88	12.0
89	16.0
90	23.0
91	13.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	729827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.39915594200125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.46311278469042	18.865658585038656
2	11.115247825556459	5.868663213617104
3	4.0298246698947455	3.191519096384252
4	1.9263160666677073	2.0341247295017313
5	1.12369447208159	1.4832292799823332
6	0.7307410851512228	1.1574568716080607
7	0.5595740812106758	1.034059840168789
8	0.4442772142837636	0.9382834769083983
9	0.38085510892441393	0.9048828070623127
>10	7.7502067108247	49.80000623171977
>50	0.35997772064943984	6.292130194521052
>100	0.10366941593227483	4.772183024781267
>500	0.009377133099401743	1.6921308339166778
>1k	0.0031257110331339138	1.9656718147895866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	3467	0.47504408579019414	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTT	2702	0.37022472448950233	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	2523	0.3456983641328698	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2105	0.2884245170430801	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1813	0.2484150353439925	No Hit
TGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTTG	1683	0.230602594861522	Illumina Single End Adapter 1 (95% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	935	0.12811255270084554	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	865	0.11852123859489988	No Hit
ACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	862	0.11811018227607364	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	812	0.1112592436289696	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	799	0.10947799958072255	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	779	0.10673762412188094	Illumina Single End Adapter 1 (95% over 22bp)
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	757	0.10372321111715517	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3759795129530697	0.0
2	0.0	0.0	0.0	1.4096491360281271	0.0
3	0.0	0.0	0.0	2.1913412356626982	0.0
4	0.0	0.0	0.0	2.682964592978884	0.0
5	0.0	0.0	0.0	5.34099176928231	0.0
6	0.0	0.0	0.0	5.846317003892703	0.0
7	0.0	0.0	0.0	8.120280559639475	0.0
8	0.0	0.0	0.0	10.500433664416361	0.0
9	0.0	0.0	0.0	12.755899685815953	0.0
10	0.0	0.0	0.0	13.845061911932554	0.0
11	0.0	0.0	0.0	14.90956075891958	0.0
12	0.0	0.0	0.0	15.838136983148061	0.0
13	1.370187729420808E-4	0.0	0.0	16.219734265791757	0.0
14	1.370187729420808E-4	0.0	0.0	16.483084347386434	0.0
15	2.740375458841616E-4	0.0	0.0	17.419607660445557	0.0
16	2.740375458841616E-4	0.0	0.0	18.38106839018014	0.0
17	2.740375458841616E-4	0.0	0.0	19.581078803606882	0.0
18	2.740375458841616E-4	0.0	0.0	20.159435044195405	0.0
19	2.740375458841616E-4	0.0	0.0	20.834389519708097	0.0
20	2.740375458841616E-4	0.0	0.0	21.626495046086266	0.0
21	2.740375458841616E-4	0.0	0.0	22.328305201095603	0.0
22	2.740375458841616E-4	0.0	0.0	23.075468569948768	0.0
23	2.740375458841616E-4	0.0	0.0	23.650673378759624	0.0
24	2.740375458841616E-4	0.0	0.0	24.145722205399363	0.0
25	2.740375458841616E-4	0.0	0.0	24.61583361536364	0.0
26	2.740375458841616E-4	0.0	0.0	25.070461903985464	0.0
27	2.740375458841616E-4	0.0	0.0	25.507688808443646	0.0
28	2.740375458841616E-4	0.0	0.0	25.943271487626518	0.0
29	4.110563188262424E-4	0.0	0.0	26.460654374255817	0.0
30	4.110563188262424E-4	0.0	0.0	26.997082870324064	0.0
31	4.110563188262424E-4	0.0	0.0	27.462946698327137	0.0
32	4.110563188262424E-4	0.0	0.0	27.919493249770152	0.0
33	5.480750917683232E-4	0.0	0.0	28.33096062491522	0.0
34	5.480750917683232E-4	0.0	0.0	28.90123275790016	0.0
35	5.480750917683232E-4	0.0	0.0	29.32270250347	0.0
36	5.480750917683232E-4	0.0	0.0	29.7837706744201	0.0
37	5.480750917683232E-4	0.0	0.0	30.201129856801682	0.0
38	5.480750917683232E-4	0.0	0.0	30.597936223241945	0.0
39	5.480750917683232E-4	0.0	0.0	31.040232822298982	0.0
40	5.480750917683232E-4	0.0	0.0	31.492942848099617	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	55	1.8189894E-12	46.000004	19
ATTCGTC	20	6.3099316E-4	46.000004	35
ATAAGCG	40	5.6061253E-9	46.000004	32
ACCGTTA	20	6.3099316E-4	46.000004	25
CGGTCTA	35	1.0188887E-7	46.000004	30
ATAACGG	35	1.0188887E-7	46.000004	1
CGACTAA	20	6.3099316E-4	46.000004	13
GCCAACG	20	6.3099316E-4	46.000004	45
TAATCGT	55	1.8189894E-12	46.000004	20
ATTCGAT	30	1.8604624E-6	46.0	13
TATTGTC	25	3.4159217E-5	46.0	6
CGTAGGG	255	0.0	46.0	2
GTCAAGC	50	1.6370905E-11	46.0	15
CGGTATT	25	3.4159217E-5	46.0	43
AGTACGG	50	1.6370905E-11	46.0	1
TAGTCAA	25	3.4159217E-5	46.0	11
TATCCGC	25	3.4159217E-5	46.0	29
AACACGG	25	3.4159217E-5	46.0	1
ATTGCGG	155	0.0	44.51613	1
ACGGGAT	120	0.0	44.083332	4
>>END_MODULE