FastQCFastQC Report
Thu 26 May 2016
SRR1544910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544910_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences444824
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT22320.5017714871499739No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT12750.28663021779400394Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT10320.2320018704026761No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.1982806683092639No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC6150.13825692858299013No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC5590.12566767980144955No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT5280.11869863136881104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT351.01767E-746.0000045
AATCAGG351.01767E-746.0000041
ACATTGG351.01767E-746.0000041
GTAATGG700.046.0000041
TACGCGG351.01767E-746.0000041
CTCGCGG351.01767E-746.0000041
CTCGAGG351.01767E-746.0000041
TACACAA351.01767E-746.00000429
GCATCGG351.01767E-746.0000041
CGGGTCA206.3072616E-446.05
AAGTAGG800.046.01
AGGTAGT206.3072616E-446.020
CAGCGAT1300.046.020
CGGAAAT206.3072616E-446.030
CGGCGAT206.3072616E-446.05
CTCACGG206.3072616E-446.01
AGTCCGG253.4137556E-546.01
TCTTGCG206.3072616E-446.020
CGTATTA206.3072616E-446.017
CACGGGT453.092282E-1046.03
Produced by FastQC (version 0.11.5)