##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544910_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	444824
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92643832167329	34.0	33.0	34.0	31.0	34.0
2	33.219689135478305	34.0	33.0	34.0	31.0	34.0
3	33.24955712821251	34.0	34.0	34.0	31.0	34.0
4	36.54069474668633	37.0	37.0	37.0	35.0	37.0
5	36.5296971386436	37.0	37.0	37.0	35.0	37.0
6	36.6573566174487	37.0	37.0	37.0	35.0	37.0
7	36.496023146233114	37.0	37.0	37.0	35.0	37.0
8	36.53030861644156	37.0	37.0	37.0	35.0	37.0
9	38.29445128859954	39.0	39.0	39.0	37.0	39.0
10	38.02324065248278	39.0	39.0	39.0	35.0	39.0
11	36.87807087747064	39.0	35.0	39.0	35.0	39.0
12	36.692024261280864	39.0	35.0	39.0	35.0	39.0
13	36.661524558027445	39.0	35.0	39.0	35.0	39.0
14	37.798039224502276	40.0	35.0	41.0	35.0	41.0
15	37.90934616837221	40.0	35.0	41.0	35.0	41.0
16	37.9372470909843	40.0	35.0	41.0	35.0	41.0
17	38.0431024405158	40.0	35.0	41.0	35.0	41.0
18	38.02669145549701	39.0	35.0	41.0	35.0	41.0
19	37.89011384277827	39.0	35.0	41.0	35.0	41.0
20	37.75627214358938	39.0	35.0	41.0	35.0	41.0
21	37.70706841357481	39.0	35.0	41.0	35.0	41.0
22	37.70288923259536	39.0	35.0	41.0	35.0	41.0
23	37.678216553063685	39.0	35.0	41.0	35.0	41.0
24	37.64175494128015	39.0	35.0	41.0	35.0	41.0
25	37.6040141718972	38.0	35.0	41.0	35.0	41.0
26	37.56026653238135	38.0	35.0	41.0	35.0	41.0
27	37.586135190547274	38.0	35.0	41.0	35.0	41.0
28	37.600974767548514	38.0	35.0	41.0	35.0	41.0
29	37.619550653741705	38.0	35.0	41.0	35.0	41.0
30	37.487475945542506	38.0	35.0	41.0	35.0	41.0
31	37.327864503713826	38.0	35.0	41.0	35.0	41.0
32	37.26501267917199	38.0	35.0	41.0	35.0	41.0
33	37.15216130424617	38.0	35.0	41.0	35.0	41.0
34	37.000499073790984	38.0	35.0	41.0	34.0	41.0
35	36.92362372533856	37.0	35.0	41.0	35.0	41.0
36	36.93065572001511	37.0	35.0	41.0	34.0	41.0
37	36.881024854774026	37.0	35.0	41.0	34.0	41.0
38	36.87523829649479	37.0	35.0	41.0	34.0	41.0
39	36.76989326115498	37.0	35.0	41.0	34.0	41.0
40	36.778802402748056	37.0	35.0	41.0	34.0	41.0
41	36.71653957520277	36.0	35.0	41.0	34.0	41.0
42	36.56811233206842	36.0	35.0	41.0	34.0	41.0
43	36.45436172508678	36.0	35.0	40.0	34.0	41.0
44	36.38140927647789	36.0	35.0	40.0	34.0	41.0
45	36.37167733755373	36.0	35.0	40.0	34.0	41.0
46	36.409822761361795	36.0	35.0	41.0	34.0	41.0
47	36.36209377191878	36.0	35.0	40.0	34.0	41.0
48	36.41358604751542	36.0	35.0	40.0	34.0	41.0
49	36.30375834037732	35.0	35.0	40.0	34.0	41.0
50	36.20792718018812	35.0	35.0	40.0	34.0	41.0
51	36.1113046957898	36.0	35.0	40.0	33.0	41.0
52	35.89708738737119	35.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	3.0
14	7.0
15	9.0
16	9.0
17	28.0
18	54.0
19	88.0
20	136.0
21	247.0
22	425.0
23	711.0
24	1225.0
25	1957.0
26	2552.0
27	3067.0
28	2838.0
29	3042.0
30	3446.0
31	4301.0
32	5749.0
33	8261.0
34	25796.0
35	141535.0
36	14231.0
37	19737.0
38	37418.0
39	166913.0
40	1035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.837526752153664	22.506879125227055	25.89338704746147	23.762207075157814
2	25.266622304551912	25.247963239393556	26.839603978202614	22.645810477851914
3	24.582756326097513	26.144947215078325	27.123986115857058	22.148310342967108
4	25.669703073575167	27.050249087279465	24.61108213585598	22.66896570328939
5	23.14510907684837	34.66539575202777	21.514126935596998	20.67536823552686
6	87.3188047407514	2.92879880581983	7.2581515385860484	2.4942449148427244
7	87.29497509127205	1.8850151970217432	5.549610632519828	5.270399079186375
8	78.63064942539071	3.3129957016707734	11.778366275200979	6.277988597737533
9	58.039809003111344	13.544233224826	14.738638202974661	13.677319569087999
10	53.290739708289124	14.546202543028253	22.333552146466918	9.829505602215708
11	48.60236857723504	14.72649857022103	21.226597485747174	15.444535366796755
12	19.087998848982966	42.07461827599231	25.32080103591533	13.516581839109401
13	10.963886840638096	45.761694512885995	27.015179037102317	16.259239609373594
14	9.802753448554933	19.44319551103358	55.909977878891425	14.844073161520061
15	9.454301026923009	14.108276531841806	57.01963023577864	19.417792205456543
16	11.314587342409583	17.435659946405767	28.43191914105354	42.81783357013111
17	18.288806359369097	20.628608168623998	38.272665143967046	22.809920328039855
18	25.228854558207292	26.145621639120193	27.36363145873424	21.26189234393828
19	28.914581947017247	20.861734079096454	24.985162671078896	25.238521302807403
20	16.318364117044045	32.872776648741976	28.16934338075284	22.639515853461145
21	19.477591137168858	23.45039835980073	24.672229915652032	32.39978058737838
22	14.695924680323005	32.81185367695988	21.536607736992607	30.955613905724512
23	16.26238692156898	21.249752711184648	41.99188892685646	20.49597144038991
24	15.071129255615704	20.608375447367948	39.01677967016168	25.303715626854668
25	12.291827779076668	33.48695214286999	30.193514738413395	24.027705339639947
26	16.152230994730502	32.09966188874701	27.14893980540618	24.599167311116307
27	16.10569573584159	27.29821232667302	40.537605884574575	16.058486052910816
28	13.923484344369907	22.719772314443464	36.30177328561409	27.054970055572543
29	17.193766523389026	28.090210959840295	29.237181447044225	25.478841069726453
30	32.00928906713666	23.288311781738397	20.620739888135535	24.081659262989408
31	30.14248331924537	22.217326403251622	24.369413520853193	23.270776756649823
32	27.170746182759924	25.767269751632107	21.048099922666044	26.01388414294193
33	20.591290038307285	23.35395572181357	27.73793680197112	28.316817437908025
34	18.38345053324461	20.97144038990702	30.29602719277737	30.349081884071005
35	32.16755390896175	18.826097512724132	26.95470568134813	22.05164289696599
36	21.341699188892687	24.741021167922593	29.03732712263727	24.87995252054745
37	20.799687067244573	30.09077747603547	23.427692750391166	25.681842706328794
38	17.668785856878223	28.716526086721938	28.33255399888495	25.28213405751488
39	23.974650648346312	23.656772116612412	26.40977105551859	25.958806179522686
40	15.053819038541086	18.33444238620218	32.4948743772818	34.11686419797493
41	22.815315720374798	22.025565167347086	24.23880006474471	30.920319047533408
42	23.257737891840367	20.886687768645576	27.996016401992698	27.859557937521355
43	22.472708307105734	26.859162275416793	28.134722946603603	22.533406470873874
44	14.897577468841607	34.16677157707318	24.63805909753071	26.297591856554504
45	24.498453320863984	28.19339783824614	24.94222434041329	22.36592450047659
46	23.018317356977143	22.28701688757801	26.834658201895582	27.86000755354927
47	23.519639228099205	18.662886894592017	31.101289498768054	26.716184378540724
48	21.388234447781596	15.398449723935759	30.952241785515174	32.261074042767476
49	19.92023811664838	25.015736560976926	30.13866158300811	24.92536373936658
50	19.177022822509578	27.770309155980794	25.680943474272972	27.37172454723666
51	19.522552739960076	20.173147132348973	32.930552308328686	27.373747819362265
52	22.84004460190997	17.957664154811788	34.22477204467385	24.97751919860439
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117.0
1	154.5
2	192.0
3	874.0
4	1556.0
5	1121.5
6	687.0
7	814.0
8	941.0
9	998.0
10	1055.0
11	1012.5
12	970.0
13	982.0
14	933.5
15	873.0
16	761.0
17	649.0
18	609.0
19	569.0
20	573.5
21	578.0
22	552.0
23	526.0
24	663.5
25	801.0
26	826.0
27	851.0
28	1280.0
29	1709.0
30	1768.0
31	1827.0
32	2035.0
33	2243.0
34	3036.0
35	3829.0
36	4644.5
37	5460.0
38	5708.0
39	7699.0
40	9442.0
41	16499.5
42	23557.0
43	34921.0
44	46285.0
45	49193.0
46	52101.0
47	50012.0
48	47923.0
49	50266.5
50	52610.0
51	53404.0
52	54198.0
53	47453.5
54	40709.0
55	33070.0
56	25431.0
57	21398.0
58	17365.0
59	14942.5
60	12520.0
61	11214.0
62	9908.0
63	9015.0
64	6240.5
65	4359.0
66	3679.5
67	3000.0
68	2326.5
69	1653.0
70	1384.5
71	1116.0
72	946.5
73	777.0
74	707.5
75	638.0
76	515.5
77	393.0
78	263.5
79	134.0
80	134.5
81	135.0
82	95.5
83	56.0
84	29.5
85	3.0
86	2.0
87	1.0
88	2.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	444824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.958521714548425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.52337230449655	15.502368092923216
2	13.089963132456148	6.010524056382656
3	5.19933792358364	3.581073378596103
4	2.5131188414871555	2.3078997397409458
5	1.4136243216317712	1.6227362342198404
6	0.9044662918859742	1.245912540140474
7	0.5690341274328997	0.9144927659691141
8	0.4542289115195732	0.8342739462798253
9	0.35040515302848785	0.7240305883224784
>10	6.641725005888335	42.52115431737163
>50	1.1440578470823064	16.85462528190348
>100	0.18967800257036493	6.248344997623714
>500	0.003993221106744525	0.5922428095466098
>1k	0.002994915830058394	1.0403212509799
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	2232	0.5017714871499739	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT	1275	0.28663021779400394	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	1032	0.2320018704026761	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	882	0.1982806683092639	No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	615	0.13825692858299013	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	559	0.12566767980144955	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	528	0.11869863136881104	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29359926622664245	0.0
2	0.0	0.0	0.0	1.2400410049817456	0.0
3	0.0	0.0	0.0	2.033388486232757	0.0
4	0.0	0.0	0.0	2.5187489883639373	0.0
5	0.0	0.0	0.0	5.2829883279679155	0.0
6	0.0	0.0	0.0	5.870411668435156	0.0
7	0.0	0.0	0.0	9.138445767314714	0.0
8	0.0	0.0	0.0	12.28823085085337	0.0
9	0.0	0.0	0.0	15.665521644515584	0.0
10	0.0	0.0	0.0	16.763483984677087	0.0
11	2.248080139560815E-4	0.0	0.0	17.8859503983598	0.0
12	2.248080139560815E-4	0.0	0.0	18.97716849810262	0.0
13	2.248080139560815E-4	0.0	0.0	19.285155477222453	0.0
14	2.248080139560815E-4	0.0	0.0	19.534017948671835	0.0
15	2.248080139560815E-4	0.0	0.0	20.471017750840783	0.0
16	2.248080139560815E-4	0.0	0.0	21.433420858586768	0.0
17	2.248080139560815E-4	0.0	0.0	23.25638904375663	0.0
18	2.248080139560815E-4	0.0	0.0	23.811440030214197	0.0
19	2.248080139560815E-4	0.0	0.0	24.601415391255866	0.0
20	2.248080139560815E-4	0.0	0.0	25.499523407010415	0.0
21	2.248080139560815E-4	0.0	0.0	26.124939301836232	0.0
22	2.248080139560815E-4	0.0	0.0	26.960101073683074	0.0
23	2.248080139560815E-4	0.0	0.0	27.47828354585184	0.0
24	2.248080139560815E-4	0.0	0.0	27.857984281423665	0.0
25	2.248080139560815E-4	0.0	0.0	28.316367821880114	0.0
26	2.248080139560815E-4	0.0	0.0	28.693370861284464	0.0
27	2.248080139560815E-4	0.0	0.0	29.09330431811233	0.0
28	2.248080139560815E-4	0.0	0.0	29.456369260651403	0.0
29	2.248080139560815E-4	0.0	0.0	30.082909195547003	0.0
30	2.248080139560815E-4	0.0	0.0	30.69056525727029	0.0
31	2.248080139560815E-4	0.0	0.0	31.09162275416794	0.0
32	2.248080139560815E-4	0.0	0.0	31.495827563260974	0.0
33	2.248080139560815E-4	0.0	0.0	31.88924158768412	0.0
34	2.248080139560815E-4	0.0	0.0	32.556022157077855	0.0
35	2.248080139560815E-4	0.0	0.0	32.9678704386454	0.0
36	2.248080139560815E-4	0.0	0.0	33.37949391219898	0.0
37	2.248080139560815E-4	0.0	0.0	33.76796216031509	0.0
38	2.248080139560815E-4	0.0	0.0	34.11866266208658	0.0
39	2.248080139560815E-4	0.0	0.0	34.570976386166215	0.0
40	4.49616027912163E-4	0.0	0.0	34.99968526878046	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.01767E-7	46.000004	5
AATCAGG	35	1.01767E-7	46.000004	1
ACATTGG	35	1.01767E-7	46.000004	1
GTAATGG	70	0.0	46.000004	1
TACGCGG	35	1.01767E-7	46.000004	1
CTCGCGG	35	1.01767E-7	46.000004	1
CTCGAGG	35	1.01767E-7	46.000004	1
TACACAA	35	1.01767E-7	46.000004	29
GCATCGG	35	1.01767E-7	46.000004	1
CGGGTCA	20	6.3072616E-4	46.0	5
AAGTAGG	80	0.0	46.0	1
AGGTAGT	20	6.3072616E-4	46.0	20
CAGCGAT	130	0.0	46.0	20
CGGAAAT	20	6.3072616E-4	46.0	30
CGGCGAT	20	6.3072616E-4	46.0	5
CTCACGG	20	6.3072616E-4	46.0	1
AGTCCGG	25	3.4137556E-5	46.0	1
TCTTGCG	20	6.3072616E-4	46.0	20
CGTATTA	20	6.3072616E-4	46.0	17
CACGGGT	45	3.092282E-10	46.0	3
>>END_MODULE