FastQCFastQC Report
Thu 26 May 2016
SRR1544909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544909_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences312704
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT21300.6811553417928776No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT13360.4272410970118707Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT11740.37543491608677854No Hit
TGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTTG8650.27661942284076957Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT5620.17972267703643063No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.17844351207531722No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC4770.15254042161277118No Hit
GCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC4380.1400685632419157No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC3860.12343941874744166No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC3530.11288630781825623No Hit
ACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT3380.10808943921408105No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG3150.10073424068767908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAGG750.046.0000041
AGGGTAT750.046.0000045
GTACGGG750.046.0000042
TTGATGG253.4114135E-546.01
AACGTGC206.304374E-446.042
GACAGTA253.4114135E-546.08
AAGTAGG253.4114135E-546.01
CGGGTAC253.4114135E-546.05
TTGGCGG453.092282E-1046.01
CTCACGG206.304374E-446.01
CAAACGT206.304374E-446.040
AAATCGA206.304374E-446.014
GTACATT206.304374E-446.026
GTATTCA206.304374E-446.08
AGTCAGG206.304374E-446.01
CACGGGT405.5879354E-946.03
CATGCGG600.046.01
TTTGTAT405.5879354E-946.040
GTTTACG253.4114135E-546.046
ACACCCG206.304374E-446.027
Produced by FastQC (version 0.11.5)