##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544909_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	312704
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9221084476054	34.0	33.0	34.0	31.0	34.0
2	33.248503376995494	34.0	33.0	34.0	31.0	34.0
3	33.359675603765865	34.0	34.0	34.0	31.0	34.0
4	36.601546510437984	37.0	37.0	37.0	35.0	37.0
5	36.59463901964797	37.0	37.0	37.0	35.0	37.0
6	36.41097011870651	37.0	37.0	37.0	35.0	37.0
7	36.24732014940647	37.0	37.0	37.0	35.0	37.0
8	36.39153640503479	37.0	37.0	37.0	35.0	37.0
9	38.258589592713875	39.0	39.0	39.0	37.0	39.0
10	38.07569138866148	39.0	39.0	39.0	35.0	39.0
11	37.08718148792468	39.0	35.0	39.0	35.0	39.0
12	36.985852435530084	39.0	35.0	39.0	35.0	39.0
13	36.95918504400328	39.0	35.0	39.0	35.0	39.0
14	38.06987758391322	40.0	35.0	41.0	35.0	41.0
15	38.14277399713467	40.0	35.0	41.0	35.0	41.0
16	38.20327210397053	40.0	35.0	41.0	35.0	41.0
17	38.322224851616866	40.0	36.0	41.0	35.0	41.0
18	38.310955408309454	40.0	36.0	41.0	35.0	41.0
19	38.21219108166189	40.0	35.0	41.0	35.0	41.0
20	38.102387561399915	40.0	35.0	41.0	35.0	41.0
21	38.058413067949246	40.0	35.0	41.0	35.0	41.0
22	38.05276555464593	40.0	35.0	41.0	35.0	41.0
23	38.004854431027425	40.0	35.0	41.0	35.0	41.0
24	37.99589707838723	40.0	35.0	41.0	35.0	41.0
25	37.95810734752354	40.0	35.0	41.0	35.0	41.0
26	37.90606771899304	40.0	35.0	41.0	35.0	41.0
27	37.99772628428162	40.0	35.0	41.0	35.0	41.0
28	38.00142626893164	40.0	35.0	41.0	35.0	41.0
29	38.020821607654526	40.0	35.0	41.0	35.0	41.0
30	37.91277374130168	40.0	35.0	41.0	35.0	41.0
31	37.77920013814982	40.0	35.0	41.0	35.0	41.0
32	37.74014083606222	40.0	35.0	41.0	35.0	41.0
33	37.65067603868195	39.0	35.0	41.0	35.0	41.0
34	37.528659690953745	39.0	35.0	41.0	35.0	41.0
35	37.46789615738846	39.0	35.0	41.0	35.0	41.0
36	37.46136282234957	39.0	35.0	41.0	35.0	41.0
37	37.4399176217765	39.0	35.0	41.0	35.0	41.0
38	37.440755474826034	39.0	35.0	41.0	35.0	41.0
39	37.379915191363075	39.0	35.0	41.0	35.0	41.0
40	37.375633186655755	39.0	35.0	41.0	35.0	41.0
41	37.32009823986901	39.0	35.0	41.0	35.0	41.0
42	37.2045096960704	39.0	35.0	41.0	35.0	41.0
43	37.124309250921	38.0	35.0	41.0	35.0	41.0
44	36.98757930822759	38.0	35.0	41.0	35.0	41.0
45	37.01282362873516	38.0	35.0	41.0	35.0	41.0
46	36.97154177752763	38.0	35.0	41.0	35.0	41.0
47	36.90999795333606	37.0	35.0	41.0	35.0	41.0
48	36.902035151453134	37.0	35.0	41.0	35.0	41.0
49	36.80302458555055	37.0	35.0	41.0	35.0	41.0
50	36.69986312934916	36.0	35.0	41.0	34.0	41.0
51	36.60298237310683	36.0	35.0	40.0	34.0	41.0
52	36.1409671766271	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	3.0
15	4.0
16	2.0
17	14.0
18	17.0
19	50.0
20	79.0
21	136.0
22	216.0
23	399.0
24	626.0
25	1133.0
26	1583.0
27	1746.0
28	1597.0
29	1553.0
30	1701.0
31	2196.0
32	2925.0
33	4781.0
34	15869.0
35	91376.0
36	9131.0
37	13405.0
38	26979.0
39	134383.0
40	798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.359074396234135	21.812960499386	24.422457020057305	24.405508084322555
2	24.82027732296357	25.306679799426934	27.165306487924685	22.70773638968481
3	24.96674171101105	26.073858984854688	27.05146080638559	21.90793849774867
4	25.7930822758903	26.807140298812936	24.789897155137126	22.60988027015964
5	23.03935990585346	34.59885386819484	21.49412863282849	20.86765759312321
6	85.48339643880475	2.7898587801882933	8.336318051575931	3.3904267294310273
7	85.17703643061809	1.922584936553418	6.412134158821122	6.488244474007368
8	75.1224800450266	3.6059660253786325	13.645492222677035	7.6260617069177234
9	54.71596142038477	13.833209680720426	15.970694330740892	15.480134568153908
10	49.8183585755219	14.668504400327468	25.131114408514122	10.382022615636513
11	45.41739152681129	14.868373925501432	22.431116966844044	17.28311758084323
12	18.683163630781827	38.61319330740892	27.86437013917315	14.839272922636104
13	11.169348649201801	42.383851821530904	28.53337341383545	17.913426115431847
14	9.894340974212035	19.77333196889071	53.76394289807613	16.56838415882112
15	9.558879963160049	14.505730659025787	55.287108575521906	20.648280802292263
16	11.114664347114205	19.112323475235364	29.440301371264837	40.33271080638559
17	17.068537658616457	21.160266577977897	38.414922738436346	23.3562730249693
18	24.178776094965208	27.110622185837087	28.056564674580436	20.654037044617272
19	27.28330945558739	21.334552803929594	25.094018624641834	26.28811911584118
20	15.4743783258289	34.38907081457225	27.550015349979535	22.58653550961932
21	18.489370139173147	23.889045231273027	24.580753683995088	33.04083094555874
22	14.614139889480146	33.637561399918134	21.58271080638559	30.165587904216128
23	15.232935939418748	21.903461420384772	41.040728612361846	21.82287402783463
24	15.865483012689316	21.17529676627098	37.390951187065085	25.568269033974623
25	12.614805055259925	32.447937986082685	30.719786123618505	24.21747083503889
26	16.658245497339337	29.829807101923866	26.716319586573885	26.795627814162913
27	17.932933381088827	26.112553724928368	39.05194688907081	16.902566004911993
28	15.10278090462546	22.74131446991404	34.86332122390504	27.292583401555465
29	17.92429901760131	26.947528653295127	27.42433739255014	27.703834936553417
30	31.982321940237412	22.276657797789603	19.782925706099057	25.958094555873924
31	30.004413119115842	20.948884568153908	23.667110110519854	25.3795922022104
32	26.490227179697097	23.86857859189521	20.967112668849776	28.67408155955792
33	21.2015196479738	21.13788119115841	28.435517294310277	29.225081866557513
34	19.24279830126893	19.087379758493654	30.475145824805566	31.194676115431847
35	30.81860161686451	19.03077670896439	27.000294207941057	23.150327466230046
36	21.930323884568153	23.70196735571019	27.745727589029883	26.62198117069177
37	21.249808125255836	29.682063548915266	24.103625153499795	24.964503172329103
38	17.29782797789603	28.274982091690543	27.49597063037249	26.931219300040933
39	24.15862924682767	22.933828796561603	27.100708657388456	25.80683329922227
40	15.643228100695866	17.502174580433895	32.52948475235367	34.32511256651658
41	22.18423812934916	21.98916547277937	24.376087290216947	31.450509107654522
42	22.404254502660663	20.068179492427344	28.774496009005325	28.75306999590667
43	23.831163016782643	25.184519545640605	28.559596295538274	22.42472114203848
44	15.111095476872697	31.0610033769955	26.57561144085141	27.25228970528039
45	23.566056078591895	27.346947912402786	25.27725900532133	23.809737003683995
46	23.377699038067952	20.141731477691362	27.81959936553418	28.66097011870651
47	22.39274201801064	17.521042263610315	32.31394545640606	27.772270261972988
48	20.462801882930822	14.74141680311093	30.855697400736798	33.94008391322145
49	19.7867632009824	22.961011051985263	31.222497953336063	26.02972779369627
50	19.8516808227589	25.30220272206304	27.103906569791242	27.74220988538682
51	18.967458043389275	18.557485673352435	33.47574703233729	28.999309250921
52	22.318550450266066	16.9127993246009	35.56942028243962	25.199229942693407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72.0
1	95.0
2	118.0
3	557.5
4	997.0
5	733.0
6	469.0
7	519.5
8	570.0
9	628.0
10	686.0
11	704.0
12	722.0
13	674.5
14	601.5
15	576.0
16	502.5
17	429.0
18	422.0
19	415.0
20	382.0
21	349.0
22	371.5
23	394.0
24	439.0
25	484.0
26	517.5
27	551.0
28	777.5
29	1004.0
30	1120.0
31	1236.0
32	1310.0
33	1384.0
34	1872.0
35	2360.0
36	2877.5
37	3395.0
38	3650.5
39	4906.5
40	5907.0
41	10277.5
42	14648.0
43	21662.5
44	28677.0
45	30579.0
46	32481.0
47	32569.5
48	32658.0
49	36664.5
50	40671.0
51	41779.5
52	42888.0
53	37431.0
54	31974.0
55	25799.5
56	19625.0
57	16054.0
58	12483.0
59	10658.5
60	8834.0
61	7884.5
62	6935.0
63	6318.5
64	4393.5
65	3085.0
66	2530.0
67	1975.0
68	1567.0
69	1159.0
70	940.5
71	722.0
72	648.5
73	575.0
74	493.5
75	412.0
76	363.0
77	314.0
78	206.0
79	98.0
80	89.5
81	81.0
82	66.5
83	52.0
84	28.0
85	4.0
86	2.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	312704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.443512075317233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.7726209749955	20.130218993041343
2	11.518483540205073	6.552522513303316
3	4.34430652995143	3.7070200573065906
4	2.0147508544702286	2.292263610315186
5	1.2311117107393414	1.750857040523946
6	0.7296726029861486	1.2452670896438804
7	0.5621514660910235	1.119269340974212
8	0.454218384601547	1.0335652885796152
9	0.3406637884511603	0.8720707122390503
>10	7.568807339449542	48.45828643061809
>50	0.38001439107753193	7.011422943102742
>100	0.0764525993883792	3.708619013507982
>500	0.0033729087965461415	0.6347856119525175
>1k	0.0033729087965461415	1.4838313548915267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	2130	0.6811553417928776	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT	1336	0.4272410970118707	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	1174	0.37543491608677854	No Hit
TGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTTG	865	0.27661942284076957	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	562	0.17972267703643063	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	558	0.17844351207531722	No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	477	0.15254042161277118	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	438	0.1400685632419157	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	386	0.12343941874744166	No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	353	0.11288630781825623	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	338	0.10808943921408105	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	315	0.10073424068767908	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43619525173966434	0.0
2	0.0	0.0	0.0	1.8052215513712648	0.0
3	0.0	0.0	0.0	2.8023306385591487	0.0
4	0.0	0.0	0.0	3.4310402169463776	0.0
5	0.0	0.0	0.0	6.9298761768317645	0.0
6	0.0	0.0	0.0	7.6257419156774455	0.0
7	0.0	0.0	0.0	11.60074703233729	0.0
8	0.0	0.0	0.0	15.39123260335653	0.0
9	0.0	0.0	0.0	19.592330126893163	0.0
10	0.0	0.0	0.0	20.980863692181742	0.0
11	0.0	0.0	0.0	22.308956713057714	0.0
12	0.0	0.0	0.0	23.634171612771183	0.0
13	0.0	0.0	0.0	24.013124232501024	0.0
14	3.197912402783463E-4	0.0	0.0	24.317565493246008	0.0
15	3.197912402783463E-4	0.0	0.0	25.45250460499386	0.0
16	3.197912402783463E-4	0.0	0.0	26.563459373720836	0.0
17	3.197912402783463E-4	0.0	0.0	28.50779011461318	0.0
18	3.197912402783463E-4	0.0	0.0	29.107718481375358	0.0
19	3.197912402783463E-4	0.0	0.0	29.883851821530904	0.0
20	3.197912402783463E-4	0.0	0.0	30.824357859189522	0.0
21	3.197912402783463E-4	0.0	0.0	31.519584015554646	0.0
22	3.197912402783463E-4	0.0	0.0	32.434826545231275	0.0
23	3.197912402783463E-4	0.0	0.0	33.01748618501842	0.0
24	3.197912402783463E-4	0.0	0.0	33.45560018419975	0.0
25	3.197912402783463E-4	0.0	0.0	33.9806974007368	0.0
26	3.197912402783463E-4	0.0	0.0	34.39930413426116	0.0
27	3.197912402783463E-4	0.0	0.0	34.856285816618914	0.0
28	3.197912402783463E-4	0.0	0.0	35.22244678673762	0.0
29	3.197912402783463E-4	0.0	0.0	35.87641987310683	0.0
30	3.197912402783463E-4	0.0	0.0	36.519839848546866	0.0
31	3.197912402783463E-4	0.0	0.0	36.9624309250921	0.0
32	3.197912402783463E-4	0.0	0.0	37.404062627916495	0.0
33	3.197912402783463E-4	0.0	0.0	37.82107040523946	0.0
34	3.197912402783463E-4	0.0	0.0	38.52652988129349	0.0
35	3.197912402783463E-4	0.0	0.0	38.95856784690954	0.0
36	3.197912402783463E-4	0.0	0.0	39.387727691363075	0.0
37	6.395824805566926E-4	0.0	0.0	39.77019801473598	0.0
38	6.395824805566926E-4	0.0	0.0	40.15394750306999	0.0
39	6.395824805566926E-4	0.0	0.0	40.632355198526405	0.0
40	6.395824805566926E-4	0.0	0.0	41.08422022103971	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAAGG	75	0.0	46.000004	1
AGGGTAT	75	0.0	46.000004	5
GTACGGG	75	0.0	46.000004	2
TTGATGG	25	3.4114135E-5	46.0	1
AACGTGC	20	6.304374E-4	46.0	42
GACAGTA	25	3.4114135E-5	46.0	8
AAGTAGG	25	3.4114135E-5	46.0	1
CGGGTAC	25	3.4114135E-5	46.0	5
TTGGCGG	45	3.092282E-10	46.0	1
CTCACGG	20	6.304374E-4	46.0	1
CAAACGT	20	6.304374E-4	46.0	40
AAATCGA	20	6.304374E-4	46.0	14
GTACATT	20	6.304374E-4	46.0	26
GTATTCA	20	6.304374E-4	46.0	8
AGTCAGG	20	6.304374E-4	46.0	1
CACGGGT	40	5.5879354E-9	46.0	3
CATGCGG	60	0.0	46.0	1
TTTGTAT	40	5.5879354E-9	46.0	40
GTTTACG	25	3.4114135E-5	46.0	46
ACACCCG	20	6.304374E-4	46.0	27
>>END_MODULE