FastQCFastQC Report
Thu 26 May 2016
SRR1544908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544908_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1307026
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT44790.3426863734921876TruSeq Adapter, Index 23 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT34530.2641875525046939TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT34500.26395802378835614TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.2025973469540774No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG18350.14039506482656045No Hit
GGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCCGAAAG17110.13090787788460215No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16920.12945419601446337No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT14180.10849057325562No Hit
ACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC14020.10726642010181894TruSeq Adapter, Index 22 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13120.10038055861168792No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA206.3117745E-446.035
CGAACGT206.3117745E-446.037
CGAACCG253.4174147E-546.012
CGAGTAC206.3117745E-446.023
ATTACGG800.046.01
GCACGTT206.3117745E-446.09
CGACAAA206.3117745E-446.022
AATGCGG2000.046.01
TACGCGG1300.046.01
ACCTACG206.3117745E-446.032
TACGCAC206.3117745E-446.016
GGACGTA206.3117745E-446.08
CGGTGAT301.861601E-646.05
AATTCGG253.4174147E-546.012
CGCGTTC206.3117745E-446.039
CAATCGT206.3117745E-446.038
TCGGTCA206.3117745E-446.038
ATACCGG501.6370905E-1146.01
TAACTCG253.4174147E-546.037
CGCTTAA206.3117745E-446.026
Produced by FastQC (version 0.11.5)