FastQCFastQC Report
Thu 26 May 2016
SRR1544907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544907_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences721966
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT33610.4655343880459744TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT26020.3604047836047681TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT21870.30292285232268557TruSeq Adapter, Index 23 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA20700.2867171030214719No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.2241102766612278No Hit
TGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTTG15130.209566655493472TruSeq Adapter, Index 23 (96% over 26bp)
GGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCCGAAAG9390.13006152644307348No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9340.12936897305413275No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG8760.12133535374242001No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT8090.1120551383306139TruSeq Adapter, Index 23 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC7920.10970045680821536TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC7760.10748428596360493TruSeq Adapter, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC7340.10166683749650261TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG351.0188705E-746.0000041
ACGGGTA351.0188705E-746.0000044
GCCCATT206.309888E-446.035
ACCGGTA301.8604351E-646.041
CGAACTA206.309888E-446.022
GCCGATG253.415884E-546.08
TACGCGG650.046.01
AACGATC206.309888E-446.046
CTACCGG206.309888E-446.01
ACCGTTG206.309888E-446.035
CCGGTAT301.8604351E-646.042
TCGACAA850.046.018
TGGCTAC206.309888E-446.032
TCGGTAC206.309888E-446.035
ATACCGG405.6061253E-946.01
TACCGGT301.8604351E-646.040
GTTGCAT206.309888E-446.044
TATCCGG501.6370905E-1146.01
CTAGCGG1500.044.4666631
TACGGGA1250.044.163
Produced by FastQC (version 0.11.5)